{"created":"2022-01-28T01:03:13.624772+00:00","id":2005150,"links":{},"metadata":{"_buckets":{"deposit":"adaff131-3fbf-411f-9a1d-92675aaac343"},"_deposit":{"id":"2005150","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2005150"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02005150","sets":["1642838403123","1642838403551:1642838407795"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"非障害性短時間脊髄虚血後モルヒネ誘発痙性対麻痺の発生機序に関する分子生物学的検討","subitem_1551255648112":"ja"},{"subitem_1551255647225":"Investigation for mechanism of morphine-inducing spastic paraplegia after a non-injurious interval of spinal cord ischemia","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"垣花, 学","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"成田, 年","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"笹良, 剛史","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Kakinohana, Manabu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Narita, Minoru","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Sasara, Takeshi","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"脊髄虚血"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"オピオイド"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"痙性対麻痺"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"spinal cord ischemia"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"opioid"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"spastic paraplegia"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"モルヒネ"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"対麻痺"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"大動脈手術"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"科研費番号: 16591551","subitem_description_type":"Other"},{"subitem_description":"平成16年度～平成18年度科学研究費補助金(基盤研究(C))研究成果報告書","subitem_description_type":"Other"},{"subitem_description":"研究概要:ラット大動脈遮断による脊髄虚血モデルを用い、脊髄虚血後のオピオイド誘発痙性対麻痺におよぼすオピオイド受容体サブタイプの影響に関する研究を行った。使用するオピオイドサブタイプは、μアゴニストとしてDAMGO、κアゴニストとしてU50488H、そしてδアゴニストとしてDPDPEを用いた。それぞれの薬物を、6分間脊髄虚血後にこも膜下腔内に投与し、その後の下肢運動機能への影響を経時的に観察した。その結果、6分間脊髄虚血後にDAMGOおよびDPDPEを投与すると下肢運動機能が悪化したが、一方U50488Hではその効果は認められなかった。脊髄病理組織学的評価では、脊髄前角細胞の暗染化(Dark-staining)がいくつかの細胞に認められた。このことから、脊髄虚血後のオピオイド痙性対麻痺の発生には、脊髄オピオイド受容体サブタイプのμ受容体とδ受容体が関与し、κ受容体は関与しないことが明らかとなった。さらに、脊髄虚血後のオピオイド誘発痙性対麻痺におよぼす増悪因子を検討する目的で、オピオイドによる鎮痛効果を増強するカリウムATPチャンネル開口薬であるニコランジルの影響を検討した。脊髄虚血は6分間とし、その後くも膜下腔内にモルヒネならびにニコランジルを同時投与し、下肢運動機能への影響を検討した。その結果、オピオイド誘発対麻痺は脊髄内でカリウムATPチャンネル開口薬であるニコランジルにより増強され、それはカリウムATPチャンネル阻害薬であるグリベンクラミドにより抑制されることが明らかとなった。このことから、オピオイド誘発痙性対麻痺では、カリウムATPチャンネルの開口がそれを増強することが示唆された。","subitem_description_type":"Other"},{"subitem_description":"要約(欧文):(1) Although intra hecal (IT) morphine after a short interval of aortic occlusion in a rodent model induced transient spastic paraparesis via opioid receptor-coupled effects in spinal cord, investigations on the relationship between the activation of opioid receptor subtypes and neurological function after spinal cord ischemia have not been reported. To determine the role of these opioid receptors in spinal mechanisms of motor dysfunction after spinal cord ischemia we investigated whether IT administration of various opioid receptor agonists can induce paraparesis after a noninjurious interval of spinal cord ischemia in rats. In Sprague-Dawley rats implanted with an IT catheter, spinal cord ischemia was induced for 6 min using an intraaortic balloon. Mu ([D-Ala^2, N-Me Phe^4, Gly-ol^5] enkephalin), kappa (U50488H) or delta (p-Pen^<2,5>] enkephalin) agonist was injected intrathecally at 30 min after reflow. A separate group of animals was used to investigate the dose-response effect on this motor dysfunction. For this purpose, three doses of mu, kappa, or delta agonist were injected intrathecally after ischemia. After IT injection, recovery of motor function was assessed periodically using the motor deficit index (0 = complete recovery; 6 = complete paraplegia). IT administration of mu and delta but not kappa agonists produced dose-dependent effects in induction of spastic paraparesis in the rat. In addition, this spasticity induced by IT mu and delta agonists was reversed completely by IT naloxone and naltrindole, respectively. These results suggested that the effect of various opioids on motor function after a noninjurious interval of spinal cord ischemia depends upon individual opioid receptor subtypes. (2) The purpose of this study is to investigate the interaction between K+ATP channel opener (nicorandil) and morphine on motor function after non-injurious interval of spinal cord ischemia in the rat. Spinal ischemia was induced by aortic occlusion for 6 min with a balloon catheter in Sprague-Dawley rats. All animals received intrathecal injection of morphine (1-60 μg) 1 h after ischemia. In addition to the intrathecal injection of morphine, group M (control animals), group MN (combination of morphine and nicorandil), and group MNG (combination of morphine, nicorandil, and glibenclamide) received intrathecal saline, nicorandil (10 μg) and both glibenclamide (10 μg) and nicorandil (10 μg) after 150 min of reperfusion, respectively. The quintal bioassay for the effect of intrathecal morphine on neurological function after ischemia was performed to calculate 50% effective dose values (ED_<50>) for inducing paraparesis at 3 h of reperfusion. The ED_<50> in the group M and group MN was 15.1± 4.9 μg and 2.9± 1.0 μg of IT morphine respectively (p < 0.05). In Group MNG, the dose-response curve shifted back to the right and the ED_<50> for inducing paraparesis was 11.6 ± 4.7 μg of IT morphine. The present study suggests that intrathecal low dose morphine combined with nicoranil could induce spastic paraparesis after non-injurious interval of spinal cord ischemia in the rat.","subitem_description_type":"Other"},{"subitem_description":"未公開：P.5以降(別刷論文のため)","subitem_description_type":"Other"},{"subitem_description":"研究報告書","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"垣花学"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"eng"}]},"item_1617186783814":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/20.500.12000/13992"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"NCID","subitem_1522646572813":"BA82699598"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-04","bibliographicIssueDateType":"Issued"}}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"16591551.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2005150/files/16591551.pdf"},"version_id":"256763b3-1c32-4f7b-84b4-8335b3093fd7"}]},"item_title":"非障害性短時間脊髄虚血後モルヒネ誘発痙性対麻痺の発生機序に関する分子生物学的検討","item_type_id":"15","owner":"1","path":["1642838403123","1642838407795"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2009-12-16"},"publish_date":"2009-12-16","publish_status":"0","recid":"2005150","relation_version_is_last":true,"title":["非障害性短時間脊髄虚血後モルヒネ誘発痙性対麻痺の発生機序に関する分子生物学的検討"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-10-31T02:30:37.728514+00:00"}