{"created":"2022-01-28T01:03:23.297595+00:00","id":2005156,"links":{},"metadata":{"_buckets":{"deposit":"0995043c-6483-41a8-b2be-9a51be5bee5d"},"_deposit":{"id":"2005156","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2005156"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02005156","sets":["1642838403123","1642838403551:1642838407795"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"良性卵巣奇形腫を利用したヒトにおける配偶子特異的メチル化機構の解析","subitem_1551255648112":"ja"},{"subitem_1551255647225":"Analysis of gamete-specific methylation mechanism in human by taking advantage of benign ovarian teratomas","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"陣野, 吉廣","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"石丸, 忠之","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Jinno, Yoshihiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ishimaru, Tadayuki","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"良性卵巣奇形腫"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"インプリンティング"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"メチレーション"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"H19"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"SNRPN"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"内在性レトロウィルス"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"benign ovarian teratoma"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"genomic imprinting"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"methylation"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"HERV-K"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"科研費番号: 10672138","subitem_description_type":"Other"},{"subitem_description":"平成10年度～平成11年度科学研究費補助金(基盤研究(C)(2))研究成果報告書","subitem_description_type":"Other"},{"subitem_description":"研究概要:刷り込み遺伝子の配偶子特異的メチル化機構を探るため、良性卵巣奇形腫(以下、奇形腫)を利用した。最初に45のDNAマーカーで25例の奇形腫の分類を行った。メチル化解析は父性メチル化の代表としてH19、母性メチル化の代表としてSNRPNを選んでサザーン法およびPCR法により行った。また、ヒト内在性レトロウィルス(HERV)K-familyを選び、活性別およびインプリンティングドメイン内外の局在別に解析することを計画した。結果および結論を以下に要約する。1.欧米の報告と異なり、meiosisIIからの奇形腫発生は25例中1例(4％)と稀である。2.奇形腫は遺伝的に異質な(heterogenousu)細胞集団から成る。3.個々の奇形腫におけるH19およびSNRPNのメチル化はきれいな逆相関関係にあり、奇形腫発生の卵形成上におけるステージが進むにつれ、両者のメチル化パターンは卵子のそれに近づく。4.ヒトH19のprimary imprintは卵形成のかなり遅い時期まで完全に消去されない。またSNRPNのprimary imprintも同様に遅くまで確立されない。5.以上より、配偶子特異的primary imprintの形成は漸進的構築過程であること、primary imprintの消去および確立と相反する二つの機構になんらかの共役した過程が存在することが示唆される。6.胎盤、精巣、肺および末血リンパ球で6種類のHERV-Kの発現が認められ、複数の組織で発現していた3つの活性型HERV-Kを単離したが、11p15.5および15q11-q13に局在するHERV-Kはコスミドライブラリーのスクリーニングからもコンピューター検索からも得ることができなかった。","subitem_description_type":"Other"},{"subitem_description":"要約(欧文):In humans, studies of female germ cells are very limited by ethics. The current study investigated the usefulness of benign ovarian teratomas as a substitute for ova in analyses of imprinted genes. Twenty-five human benign ovarian teratomas were typed with 45 microsatellite DNA markers and classified according to their genotypic features. Two oppositely imprinted genes, H19 and SNRPN, were then chosen for analysis of their methylation states in these tumors. These analyses revealed that benign ovarian teratomas consist of a mixture of genetically and epigenetically heterogeneous cell populations. In contrast to previous reports, we could document only one case rising from germ cells by meiosis-II nondisjunction. H19 and SNRPN were methylated in individual teratomas to various degrees, ranging from normal somatic cell to expected ovum levels. The allele with residual methylation of H19 was consistent with that methylated in the patient's blood DNA, thus being of paternal origin. Degrees of H19 hypomethylation and SNRPN hypermethylation increased as the cellular origin of the tumors advanced in oogenesis and were closely correlated in individual teratomas. These results could be best explained by the assumption that the primary imprinting is a progressively organized process and suggest that the establishment of primary imprints on different genes might be mechanistically linked, even when those genes are oppositely imprinted. Transcripts of six genes of human endogenous retrovirus K (HERV-K)-family members were detected by sequencing of RT-PCR clones in the placenta, testis, peripheral blood leukocytes and fetal lung. Three of them, which were expressed at least in the two tissues analysed, were isolated from a BAC library.","subitem_description_type":"Other"},{"subitem_description":"未公開：P.7以降(別刷論文のため)","subitem_description_type":"Other"},{"subitem_description":"研究報告書","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"陣野吉廣"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"jpn"}]},"item_1617186783814":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/20.500.12000/14310"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"NCID","subitem_1522646572813":"BA46530571"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2000-03","bibliographicIssueDateType":"Issued"}}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"10672138.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2005156/files/10672138.pdf"},"version_id":"690a9e11-be12-4974-a331-8b86510de5df"}]},"item_title":"良性卵巣奇形腫を利用したヒトにおける配偶子特異的メチル化機構の解析","item_type_id":"15","owner":"1","path":["1642838403123","1642838407795"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2009-12-25"},"publish_date":"2009-12-25","publish_status":"0","recid":"2005156","relation_version_is_last":true,"title":["良性卵巣奇形腫を利用したヒトにおける配偶子特異的メチル化機構の解析"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-10-31T02:30:50.724457+00:00"}