{"created":"2022-01-28T01:05:34.335725+00:00","id":2005221,"links":{},"metadata":{"_buckets":{"deposit":"9c27f420-56e5-44d3-b630-4c703681efd1"},"_deposit":{"id":"2005221","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2005221"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02005221","sets":["1642838163960:1642838338003","1642838403551:1642838407795"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"Helicobacter pylori-Induced Interleukin-12 p40 Expression","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takeshima, Eriko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Tomimori, Koh","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Teruya, Hiromitsu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ishikawa, Chie","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Senba, Masachika","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"D' Ambrosio, Daniele","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kinjo, Fukunori","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Mimuro, Hitomi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Sasakawa, Chihiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hirayama, Toshiya","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Fujita, Jiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Mori, Naoki","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_1522650717957":"en","subitem_1522651041219":"Copyright (c) 2009, American Society for Microbiology. All Rights Reserved."}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Interleukin-12 (IL-12) is a heterodimeric cytokine produced by antigen-presenting cells that promotes the development of T-helper lymphocyte 1 (Th1). Chronic gastritis induced by Helicobacter pylori is considered a Th1-mediated process. IL-12 levels in gastric biopsy samples of H. pylori-infected patients are higher than in those of uninfected individuals, but the cellular source of IL-12 remains elusive. IL-12 staining was detected in mucosal epithelial cells, lymphocytes, and macrophages in specimens of patients with H. pylori-positive gastritis. Therefore, we investigated IL-12 p40 mRNA induction by H. pylori in gastric epithelial cells and T cells. Although cag pathogenicity island (PAI)-positive H. pylori induced IL-12 p40 mRNA expression, an isogenic mutant of the cag PAI failed to induce it in both cell types. Supernatants from H. pylori cultures and H. pylori VacA induced IL-12 p40 mRNA expression in T cells but not in epithelial cells. The activation of the IL-12 p40 promoter by H. pylori was mediated through NF-κB. The transfection of IκB kinase and NF-κB-inducing kinase dominant-negative mutants inhibited H. pylori-induced IL-12 p40 activation. Inhibitors of NF-κB, phosphatidylinositol 3-kinase, p38 mitogen-activated protein kinase, and Hsp90 suppressed H. pylori- and VacA-induced IL-12 p40 mRNA expression. The results indicate that H. pylori induces IL-12 p40 expression by the activation of NF-κB, phosphatidylinositol 3-kinase, and p38 mitogen-activated protein kinase. Hsp90 is also a crucial regulator of H. pylori-induced IL-12 p40 expression. In addition to the cag PAI, VacA might be relevant in the induction of IL-12 expression and a Th1-polarized response only in T cells.","subitem_description_type":"Other"},{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"en","subitem_1522300316516":"American Society for Microbiology"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"eng"}]},"item_1617186783814":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/20.500.12000/14407"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"0019-9567"},{"subitem_1522646500366":"NCID","subitem_1522646572813":"AA00673732"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"en","subitem_1522650091861":"Infection and immunity"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"1348","bibliographicPageStart":"1337","bibliographicVolumeNumber":"77"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"AM","subitem_1600292170262":"http://purl.org/coar/version/c_ab4af688f83e57aa"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_1522306287251":{"subitem_1522306382014":"URI","subitem_1522306436033":"http://iai.asm.org/cgi/content/abstract/77/4/1337"}},{"subitem_1522306287251":{"subitem_1522306382014":"DOI","subitem_1522306436033":"10.1128/IAI.01456-08"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"IAI01456-08R2FIG.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2005221/files/IAI01456-08R2FIG.pdf"},"version_id":"6cb4692b-35a4-4df0-9b9d-f245e2b28f7d"},{"accessrole":"open_access","filename":"IAI01456-08R2.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2005221/files/IAI01456-08R2.pdf"},"version_id":"5bda5446-b585-41ab-a9bc-d86fbb08cacf"}]},"item_title":"Helicobacter pylori-Induced Interleukin-12 p40 Expression","item_type_id":"15","owner":"1","path":["1642838338003","1642838407795"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2010-01-04"},"publish_date":"2010-01-04","publish_status":"0","recid":"2005221","relation_version_is_last":true,"title":["Helicobacter pylori-Induced Interleukin-12 p40 Expression"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-08-03T05:25:16.167754+00:00"}