{"created":"2022-01-28T07:18:08.756897+00:00","id":2008827,"links":{},"metadata":{"_buckets":{"deposit":"453b914f-d803-4c1f-914d-0eb3726bae0a"},"_deposit":{"id":"2008827","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2008827"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02008827","sets":["1642838163960:1642838338003","1642838403551:1642838412624"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"Cirsium brevicaule A. GRAY leaf inhibits adipogenesis in 3T3-L1 cells and C57BL/6 mice","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Inafuku, Masashi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ruwani N, Nugara","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kamiyama, Yasuo","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Futenma, Itsuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Inafuku, Ayako","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Oku, Hirosuke","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Cirsium brevicaule A. GRAY"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Anti-adipogenesis"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Obesity"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Non-alcoholic fatty liver disease"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Background: Various flavonoids obtained from the genus Cirsium have been reported to exhibit beneficial effects on health. The present study evaluated the antiobesity effects of Cirsium brevicaule A. GRAY leaf (CL) by using 3T3-L1 cells and C57BL/6 mice that were fed a high-fat diet (HFD). Methods: Dried CL powder was serially extracted with solvents of various polarities, and these extracts were tested for antiadipogenic activity using 3T3-L1 adipocytes. Mice were fed experimental HFD supplemented with dried CL powder for 4 wk. Lipid levels and mRNA levels of genes related to lipid metabolism were determined in 3T3-L1 adipocytes and the white adipose tissue (WAT) and liver of mice fed on a HFD. Results: Treatment of 3T3-L1 adipocytes with a hexane extract of CL significantly reduced cellular lipid accumulation and expression of the fatty acid synthase (FASN) gene. Dietary CL reduced the serum levels of nonesterified fatty acids in HFD-fed mice. Significant decreases in subcutaneous WAT weight and associated FASN gene expression were observed in the mice fed the experimental CL diet. Dietary CL also reduced the hepatic lipid and\\nserum levels of a hepatopathic indicator in the HFD-fed mice. A significant reduction in mRNA levels of FASN and HMG-CoA reductase were observed in the livers of the CL-diet group. Dietary CL, on the other hand, increased in the hepatic mRNA levels of genes related to β-oxidation, namely peroxisome proliferator-activated receptor α, calnitine palmitoyltrasferase 1A, and uncoupling protein 2. Expression of the insulin receptor gene was also significantly increased in the livers of mice-fed the CL diet. Conclusions: The present study therefore demonstrated that CL suppresses lipid accumulation in the WAT and liver partly through inhibiting mRNA levels of FASN gene and enhancing the lipolysis-related gene expression.","subitem_description_type":"Other"},{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"en","subitem_1522300316516":"BioMed Central"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"jpn"}]},"item_1617186783814":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/20.500.12000/37587"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"1476-511X"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"en","subitem_1522650091861":"Lipids in Health and Disease"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-08-15","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"132","bibliographicPageStart":"124","bibliographicVolumeNumber":"12"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_1522306287251":{"subitem_1522306382014":"DOI","subitem_1522306436033":"https://doi.org/10.1186/1476-511X-12-124"}},{"subitem_1522306287251":{"subitem_1522306382014":"DOI","subitem_1522306436033":"info:doi/10.1186/1476-511X-12-124"}},{"subitem_1522306287251":{"subitem_1522306382014":"PMID","subitem_1522306436033":"23945333"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"Vol12p124.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2008827/files/Vol12p124.pdf"},"version_id":"7313103f-ad31-4595-aee6-ab041dccebb4"}]},"item_title":"Cirsium brevicaule A. GRAY leaf inhibits adipogenesis in 3T3-L1 cells and C57BL/6 mice","item_type_id":"15","owner":"1","path":["1642838338003","1642838412624"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-01-18"},"publish_date":"2018-01-18","publish_status":"0","recid":"2008827","relation_version_is_last":true,"title":["Cirsium brevicaule A. GRAY leaf inhibits adipogenesis in 3T3-L1 cells and C57BL/6 mice"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-08-03T05:41:40.644799+00:00"}