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Ivy gourd (Coccinia grandis L. Voigt) root suppresses adipocyte differentiation in 3T3-L1 cells

http://hdl.handle.net/20.500.12000/37590
http://hdl.handle.net/20.500.12000/37590
4f53fd91-9ebc-4258-969a-840599f32204
名前 / ファイル ライセンス アクション
Vol13p88.pdf Vol13p88.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2018-01-18
タイトル
タイトル Ivy gourd (Coccinia grandis L. Voigt) root suppresses adipocyte differentiation in 3T3-L1 cells
言語 en
作成者 Bunkrongcheap, Ruthaiwan

× Bunkrongcheap, Ruthaiwan

en Bunkrongcheap, Ruthaiwan

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Hutadilok-Towatana, Nongporn

× Hutadilok-Towatana, Nongporn

en Hutadilok-Towatana, Nongporn

Search repository
Noipha, Kusumarn

× Noipha, Kusumarn

en Noipha, Kusumarn

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Wattanapiromsakul, Chatchai

× Wattanapiromsakul, Chatchai

en Wattanapiromsakul, Chatchai

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Inafuku, Masashi

× Inafuku, Masashi

en Inafuku, Masashi

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Oku, Hirosuke

× Oku, Hirosuke

en Oku, Hirosuke

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
主題
言語 en
主題Scheme Other
主題 Anti-adipogenesis
主題
言語 en
主題Scheme Other
主題 Adipocyte
主題
言語 en
主題Scheme Other
主題 Ivy gourd
主題
言語 en
主題Scheme Other
主題 Obesity
主題
言語 en
主題Scheme Other
主題 3T3-L1 cells
内容記述
内容記述タイプ Other
内容記述 Background: Ivy gourd (Coccinia grandis L. Voigt) is a tropical plant widely distributed throughout Asia, Africa, and the Pacific Islands. The anti-obesity property of this plant has been claimed but still remains to be scientifically proven. We therefore investigated the effects of ivy gourd leaf, stem, and root on adipocyte differentiation by employing cell culture model. Methods: Dried roots, stems, and leaves of ivy gourd were separately extracted with ethanol. Each extract was then applied to 3T3-L1 pre-adipocytes upon induction with a mixture of insulin, 3-isobutyl-1-methylxanthine, and dexamethasone, for anti-adipogenesis assay. The active extract was further fractionated by a sequential solvent partitioning method, and the resulting fractions were examined for their abilities to inhibit adipogenesis in 3T3-L1 cells. Differences in the expression of adipogenesis-related genes between the treated and untreated cells were determined from their mRNA and protein levels. Results: Of the three ivy gourd extracts, the root extract exhibited an anti-adipogenic effect. It significantly reduced intracellular fat accumulation during the early stages of adipocyte differentiation. Together with the suppression of differentiation, expression of the genes encoding PPARγ, C/EBPα, adiponectin, and GLUT4 were down-regulated. Hexane-soluble fraction of the root extract also inhibited adipocyte differentiation and decreased the mRNA levels of various adipogenic genes in the differentiating cells. Conclusions: This is the first study to demonstrate that ivy gourd root may prevent obesity based mainly on the ability of its active constituent(s) to suppress adipocyte differentiation in vitro. Such an inhibitory effect is mediated by at least down-regulating the expression of PPARγ-the key transcription factor of adipogenesis in pre-adipocytes during their early differentiation processes.
内容記述
内容記述タイプ Other
内容記述 論文
出版者
言語 en
出版者 Lipids in Health and Disease
言語
言語 jpn
資源タイプ
資源タイプ journal article
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
識別子
識別子 http://hdl.handle.net/20.500.12000/37590
識別子タイプ HDL
関連情報
識別子タイプ DOI
関連識別子 https://doi.org/10.1186/1476-511X-13-88
関連情報
識別子タイプ DOI
関連識別子 info:doi/10.1186/1476-511X-13-88
関連情報
識別子タイプ PMID
関連識別子 24884680
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 1476-511X
収録物名
言語 en
収録物名 Lipids in Health and Disease
書誌情報
巻 13, p. 88-97, 発行日 2014-05-28
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