@article{oai:u-ryukyu.repo.nii.ac.jp:02008841,
 author = {Kawagucci, Shinsuke and Toki, Tomohiro and Ishibashi, Junichiro and Takai, Ken and Ito, Michihiro and Oomori, Tamotsu and Gamo, Toshitaka},
 issue = {G3},
 journal = {Journal of Geophysical Research: Biogeosciences},
 month = {Aug},
 note = {[1] Molecular hydrogen (H2) is one of the most important energy sources for subseafloor chemolithoautotrophic microbial ecosystems in the deep-sea hydrothermal environments. This study investigated stable isotope ratios of H2 in 20°–375°C hydrothermal fluids to evaluate usefulness of the isotope ratio as a tracer to explore the H2-metabolisms. Prior to the observation, we developed an improved analytical method for the determination of concentration and stable isotope ratio of H2. This method achieved a relatively high sensitivity with a detection limit of 1 nmol H2 within an analytical error of 10‰ in the δDH2 value. The δDH2 values in the high-temperature fluids were between −405‰ and −330‰, indicating the achievement of the hydrogen isotopic equilibrium between H2 and H2O at around the hydrothermal end-member temperature. In contrast, several low-temperature fluids showed apparently smaller δDH2 values than those in the high-temperature fluids in spite of a negligible δDH2 change due to fluid-seawater mixing, suggesting the possibility of δDH2 change in the low-temperature fluids and the surrounding environments. Since the δDH2 change in low-temperature environments is not well explained by the very sluggish abiotic thermal isotopic equilibrium between H2 and H2O, it could be associated with (micro)biological H2-consuming and/or H2-generating metabolisms that would strongly promote the isotopic equilibrium at low temperatures. Our first detection of the δDH2 variation in deep-sea hydrothermal systems presents the availability of the δDH2 value as a new tracer for microbes whose enzymes catalyze D/H exchange in H2., 論文},
 title = {Isotopic variation of molecular hydrogen in 20°–375°C hydrothermal fluids as detected by a new analytical method},
 volume = {115},
 year = {2010}
}