@article{oai:u-ryukyu.repo.nii.ac.jp:02008859,
 author = {Mino, Sayaka and Makita, Hiroko and Toki, Tomohiro and Miyazaki, Junichi and Kato, Shingo and Watanabe, Hiromi and Imachi, Hiroyuki and Watsuji, Tomo-o and Nunoura, Takuro and Kojima, Shigeaki and Sawabe, Tomoo and Takai, Ken and Nakagawa, Satoshi},
 issue = {107},
 journal = {Frontiers in microbiology},
 month = {Feb},
 note = {Deep-sea hydrothermal vent fields are areas on the seafloor with high biological productivity fueled by microbial chemosynthesis. Members of the Aquificales genus Persephone/la are obligately chemosynthetic bacteria, and appear to be key players in carbon, sulfur, and nitrogen cycles in high temperature habitats at deep-sea vents. Although this group of bacteria has cosmopolitan distribution in deep-sea hydrothermal ecosystem around the world, little is known about their population structure such as intraspecific genomic diversity, distribution pattern, and phenotypic diversity. We developed the multi-locus sequence analysis (M LSA) scheme for their genomic characterization. Sequence variation was determined in five housekeeping genes and one functional gene of 36 Persephone/la hydrogeniphila strains originated from the Okinawa Trough and the South Mariana Trough (SNT). Although the strains share >98.7% similarities in 16S rRNA gene sequences, MLSA revealed 35 different sequence types (ST), indicating their extensive genomic diversity. A phylogenetic tree inferred from all concatenated gene sequences revealed the clustering of isolates according to the geographic origin. In addition, the phenotypic clustering pattern inferred from whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF/MS) analysis can be correlated to their MLSA clustering pattern. This study represents the first MLSA combined with phenotypic analysis indicative of allopatric speciation of deep-sea hydrothermal vent bacteria., 論文},
 title = {Biogeography of Persephonella in deep-sea hydrothermal vents of the Western Pacific},
 volume = {4},
 year = {2013}
}