@article{oai:u-ryukyu.repo.nii.ac.jp:02011302,
 author = {Tsuchida, Akio and Senda, Motohiro and Ito, Akihiro and Saito, Seiichi and Kiso, Makoto and Ando, Takayuki and Harduin-Lepers, Anne and Matsuda, Akio and Furukawa, Keiko and Furukawa, Koichi},
 journal = {Scientific Reports},
 month = {May},
 note = {GalNAc-disialyl Lc4 (GalNAc-DSLc4) was reported as a novel antigen that associated with malignant features of renal cell cancers (RCCs). To clarify roles of GalNAc-DSLc4 in malignant properties of RCCs, we identified B4GalNAc-T2 as a responsible gene for the synthesis of GalNAc-DSLc4, and prepared stable transfectants of GalNAc-T2 cDNA using VMRC-RCW cells, resulting in the establishment of high expressants of GalNAc-DSLc4. They showed increased proliferation and invasion, and specific adhesion to laminin. In the transfectants, PI3K/Akt signals were highly activated by serum stimulation or adhesion to laminin. GalNAc-DSLc4 was co-localized in lipid rafts with integrin β1 and caveolin-1 in both immunoblotting of fractionated detergent extracts and immunocytostaining, particularly when stimulated with serum. Masking of GalNAc-DSLc4 with antibodies as well as PI3K inhibitor suppressed malignant properties of the transfectants. These results suggested that GalNAc-DSLc4 is involved in malignant properties of RCCs by forming a molecular complex with integrins in lipid rafts., 論文},
 title = {Roles of GalNAc-disialyl Lactotetraosyl Antigens in Renal Cancer Cells},
 volume = {8},
 year = {2018}
}