@article{oai:u-ryukyu.repo.nii.ac.jp:02011320, author = {Uehara, Fuminari and Hiroshima, Yukihiko and Miwa, Shinji and Tome, Yasunori and Yano, Shuya and Yamamoto, Mako and Matsumoto, Yasunori and Maehara, Hiroki and Tanaka, Kazuhiro and Bouvet, Michael and Kanaya, Fuminori and Hoffman, Robert M.}, issue = {2}, journal = {PLoS ONE}, month = {Feb}, note = {The aim of this study is to determine if fluorescence-guided surgery (FGS) can eradicate human fibrosarcoma growing in the retroperitoneum of nude mice. One week after retroperitoneal implantation of human HT1080 fibrosarcoma cells, expressing green fluorescent protein (GFP) (HT-1080-GFP), in nude mice, bright-light surgery (BLS) was performed on all tumor-bearing mice (n = 22). After BLS, mice were randomized into 2 treatment groups; BLS-only (n = 11) or the combination of BLS + FGS (n = 11). The residual tumors remaining after BLS were resected with FGS using a hand-held portable imaging system under fluorescence navigation. The average residual tumor area after BLS + FGS was significantly smaller than after BLS-only (0.4 ± 0.4 mm^2 and 10.5 ± 2.4 mm^2, respectively; p = 0.006). Five weeks after surgery, the fluorescent-tumor areas of BLS- and BLS + FGS-treated mice were 379 ± 147 mm^2 and 11.7 ± 6.9 mm^2, respectively, indicating that FGS greatly inhibited tumor recurrence compared to BLS. The combination of BLS + FGS significantly decreased fibrosarcoma recurrence compared to BLS-only treated mice (p < 0.001). Mice treated with BLS+FGS had a significantly higher disease-free survival rate than mice treated with BLS-only at five weeks after surgery. These results suggest that combination of BLS + FGS significantly reduced the residual fibrosarcoma volume after BLS and improved disease-free survival., 論文}, title = {Fluorescence-Guided Surgery of Retroperitoneal-Implanted Human Fibrosarcoma in Nude Mice Delays or Eliminates Tumor Recurrence and Increases Survival Compared to Bright-Light Surgery}, volume = {10}, year = {2015} }