{"created":"2022-02-02T02:04:45.450227+00:00","id":2012290,"links":{},"metadata":{"_buckets":{"deposit":"14ce71bf-b086-4651-81dd-8b20012f12b1"},"_deposit":{"id":"2012290","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2012290"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02012290","sets":["1642838163960:1642838338003","1642838403551:1642838407795"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"HTLV-1 Tax Induces Formation of the Active Macromolecular IKK Complex by Generating Lys63- and Met1-Linked Hybrid Polyubiquitin Chains","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shibata, Yuri","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Tokunaga, Fuminori","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Goto, Eiji","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Komatsu, Ginga","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Gohda, Jin","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Saeki, Yasushi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Tanaka, Keiji","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Takahashi, Hirotaka","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Sawasaki, Tatsuya","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Inoue, Satoshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Oshiumi, Hiroyuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Seya, Tsukasa","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nakano, Hiroyasu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Tanaka, Yuetsu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Iwai, Kazuhiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Inoue, Jun-ichiro","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_1522650717957":"en","subitem_1522651041219":"Creative Commons Attribution License 4.0"},{"subitem_1522650717957":"en","subitem_1522650727486":"https://creativecommons.org/licenses/by/4.0/","subitem_1522651041219":"https://creativecommons.org/licenses/by/4.0/"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"The Tax protein of human T-cell leukemia virus type 1 (HTLV-1) is crucial for the development of adult T-cell leukemia (ATL), a highly malignant CD4+ T cell neoplasm. Among the multiple aberrant Tax-induced effects on cellular processes, persistent activation of transcription factor NF-κB, which is activated only transiently upon physiological stimulation, is essential for leukemogenesis. We and others have shown that Tax induces activation of the IκB kinase (IKK) complex, which is a critical step in NF-κB activation, by generating Lys63- linked polyubiquitin chains. However, the molecular mechanism underlying Tax-induced IKK activation is controversial and not fully understood. Here, we demonstrate that Tax recruits linear (Met1-linked) ubiquitin chain assembly complex (LUBAC) to the IKK complex and that Tax fails to induce IKK activation in cells that lack LUBAC activity. Mass spectrometric analyses revealed that both Lys63-linked and Met1-linked polyubiquitin chains are associated with the IKK complex. Furthermore, treatment of the IKK-associated polyubiquitin chains with Met1-linked-chain-specific deubiquitinase (OTULIN) resulted in the reduction of high molecular weight polyubiquitin chains and the generation of short Lys63-linked ubiquitin chains, indicating that Tax can induce the generation of Lys63- and Met1-linked hybrid polyubiquitin chains. We also demonstrate that Tax induces formation of the active macromolecular IKK complex and that the blocking of Tax-induced polyubiquitin chain synthesis inhibited formation of the macromolecular complex. Taken together, these results lead us to propose a novel model in which the hybrid-chain-dependent oligomerization of the IKK complex triggered by Tax leads to trans-autophosphorylation-mediated IKK activation.","subitem_description_type":"Other"},{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"en","subitem_1522300316516":"Public Library of Science"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"eng"}]},"item_1617186783814":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/20.500.12000/45642"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"1553-7374"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"en","subitem_1522650091861":"PLOS Pathogens"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-01-19","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicVolumeNumber":"13"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_1522306287251":{"subitem_1522306382014":"DOI","subitem_1522306436033":"https://doi.org/10.1371/journal.ppat.1006162"}},{"subitem_1522306287251":{"subitem_1522306382014":"DOI","subitem_1522306436033":"https://doi.org/10.1371/journal.ppat.1006162"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"journal.ppat.1006162.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2012290/files/journal.ppat.1006162.pdf"},"version_id":"643bff3c-d2fc-4fe4-abc3-abf3cdb895e4"}]},"item_title":"HTLV-1 Tax Induces Formation of the Active Macromolecular IKK Complex by Generating Lys63- and Met1-Linked Hybrid Polyubiquitin Chains","item_type_id":"15","owner":"1","path":["1642838338003","1642838407795"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-04-15"},"publish_date":"2020-04-15","publish_status":"0","recid":"2012290","relation_version_is_last":true,"title":["HTLV-1 Tax Induces Formation of the Active Macromolecular IKK Complex by Generating Lys63- and Met1-Linked Hybrid Polyubiquitin Chains"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-08-03T05:31:28.522180+00:00"}