{"created":"2022-02-02T02:10:39.730499+00:00","id":2012472,"links":{},"metadata":{"_buckets":{"deposit":"a26af701-68f3-4204-9b7f-c07c918b0ae7"},"_deposit":{"id":"2012472","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2012472"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02012472","sets":["1642838163960:1642838338003","1642838403551:1642838407795"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"Epigenetic changes around the pX region and spontaneous HTLV-1 transcription are CTCF-independent","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Miura, Michi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Miyazato, Paola","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Satou, Yorifumi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Tanaka, Yuetsu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Bangham, Charles R.M.","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_1522650717957":"en","subitem_1522651041219":"Creative Commons Attribution 4.0"},{"subitem_1522650717957":"en","subitem_1522650727486":"https://creativecommons.org/licenses/by/4.0/","subitem_1522651041219":"https://creativecommons.org/licenses/by/4.0/"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Histone modifications"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"DNA methylation"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Epigenetics"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Antisense transcription"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Transcription kinetics"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Retrovirus"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"HTLV-1"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"CTCF"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"CRISPR/Cas9"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Single-molecule RNA-FISH"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Background: The human retrovirus HTLV-1 inserts the viral complementary DNA of 9 kb into the host genome. Both plus- and minus-strands of the provirus are transcribed, respectively from the 5′ and 3′ long terminal repeats (LTR). Plus-strand expression is rapid and intense once activated, whereas the minus-strand is transcribed at a lower, more constant level. To identify how HTLV-1 transcription is regulated, we investigated the epigenetic modifications associated with the onset of spontaneous plus-strand expression and the potential impact of the host factor CTCF.\\nMethods: Patient-derived peripheral blood mononuclear cells (PBMCs) and in vitro HTLV-1-infected T cell clones were examined. Cells were stained for the plus-strand-encoded viral protein Tax, and sorted into Tax+ and Tax– populations. Chromatin immunoprecipitation and methylated DNA immunoprecipitation were performed to identify epigenetic modifications in the provirus. Bisulfite-treated DNA fragments from the HTLV-1 LTRs were sequenced. Single-molecule RNA-FISH was performed, targeting HTLV-1 transcripts, for the estimation of transcription kinetics. The CRISPR/Cas9 technique was applied to alter the CTCF-binding site in the provirus, to test the impact of CTCF on the epigenetic modifications.\\nResults: Changes in the histone modifications H3K4me3, H3K9Ac and H3K27Ac were strongly correlated with plus-strand expression. DNA in the body of the provirus was largely methylated except for the pX and 3′ LTR regions, regardless of Tax expression. The plus-strand promoter was hypomethylated when Tax was expressed. Removal of CTCF had no discernible impact on the viral transcription or epigenetic modifications.\\nConclusions: The histone modifications H3K4me3, H3K9Ac and H3K27Ac are highly dynamic in the HTLV-1 provirus: they show rapid change with the onset of Tax expression, and are reversible. The HTLV-1 provirus has an intrinsic pattern of epigenetic modifications that is independent of both the provirus insertion site and the chromatin architectural protein CTCF which binds to the HTLV-1 provirus.","subitem_description_type":"Other"},{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"en","subitem_1522300316516":"F1000Research"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"eng"}]},"item_1617186783814":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/20.500.12000/46085"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"2398-502X"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"en","subitem_1522650091861":"Wellcome Open Research"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-12-11","bibliographicIssueDateType":"Issued"},"bibliographicVolumeNumber":"3"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_1522306287251":{"subitem_1522306382014":"DOI","subitem_1522306436033":"https://doi.org/10.12688/wellcomeopenres.14741.2"}},{"subitem_1522306287251":{"subitem_1522306382014":"DOI","subitem_1522306436033":"https://doi.org/10.12688/wellcomeopenres.14741.2"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"b03c4970-e75a-409a-b692-d05c9b7c59a2_14741_-_charles_bangham_v2.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2012472/files/b03c4970-e75a-409a-b692-d05c9b7c59a2_14741_-_charles_bangham_v2.pdf"},"version_id":"70fefe79-4635-4e2f-ba22-800b8657312c"}]},"item_title":"Epigenetic changes around the pX region and spontaneous HTLV-1 transcription are CTCF-independent","item_type_id":"15","owner":"1","path":["1642838338003","1642838407795"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-06-09"},"publish_date":"2020-06-09","publish_status":"0","recid":"2012472","relation_version_is_last":true,"title":["Epigenetic changes around the pX region and spontaneous HTLV-1 transcription are CTCF-independent"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-08-03T05:27:04.982466+00:00"}