@article{oai:u-ryukyu.repo.nii.ac.jp:02012535,
 author = {Isomura, Minoru and Ikegawa, Shiro and Kinjo, Takao and Takeuchi, Kumiko and Yamane-Tanaka, Yuka and Kitami, Kikuko and Nakamura, Yusuke},
 issue = {6},
 journal = {DNA Research},
 month = {Dec},
 note = {Large-scale sequencing of genomic regions and in silico gene trapping together represent a highly efficient and powerful approach for identifying novel genes. We performed megabase-level sequence analyses of two genomic regions on human chromosome 8p (8p11.2 and 8p22.→p21.3), after covering those segments with sequence-ready contigs composed of 74 cosmids, 14 BACs, and three PAC clones. We determined continuous nucleotide sequences of 1,856,753 bases on 8p11.2 and 1,210,381 bases on 8p22→p21.3 by combining the shotgun and primer-walking methods. In silico gene trapping identified four novel genes in the 8p11.2 region and, in the 8p22→p21.3 region, six known genes ( PRLTS, PCM1, MTMR7, HCAT2, HFREP-1 and PHP ) and three novel genes. The distribution of Alu and LINE1 repetitive elements and the densities of predicted exons were different in each region, and Alu -rich portions contained more exonic sequences than LINE1-rich areas., 論文},
 pages = {387--400},
 title = {Sequence Analysis of a Total of Three Megabases of DNA in Two Regions of Chromosome 8p},
 volume = {6},
 year = {1999}
}