{"created":"2022-02-02T02:12:38.831016+00:00","id":2012535,"links":{},"metadata":{"_buckets":{"deposit":"d2060c1f-898d-4905-b731-000adb235eec"},"_deposit":{"id":"2012535","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2012535"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02012535","sets":["1642838163960:1642838338003","1642838403551:1642838407795"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"Sequence Analysis of a Total of Three Megabases of DNA in Two Regions of Chromosome 8p","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Isomura, Minoru","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ikegawa, Shiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kinjo, Takao","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Takeuchi, Kumiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yamane-Tanaka, Yuka","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kitami, Kikuko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nakamura, Yusuke","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"large-scale DNA sequencing"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"physical and transcriptional maps"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"human chromosome 8p11.2"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"human chromosome 8p22→p21.3"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Large-scale sequencing of genomic regions and in silico gene trapping together represent a highly efficient and powerful approach for identifying novel genes. We performed megabase-level sequence analyses of two genomic regions on human chromosome 8p (8p11.2 and 8p22.→p21.3), after covering those segments with sequence-ready contigs composed of 74 cosmids, 14 BACs, and three PAC clones. We determined continuous nucleotide sequences of 1,856,753 bases on 8p11.2 and 1,210,381 bases on 8p22→p21.3 by combining the shotgun and primer-walking methods. In silico gene trapping identified four novel genes in the 8p11.2 region and, in the 8p22→p21.3 region, six known genes ( PRLTS, PCM1, MTMR7, HCAT2, HFREP-1 and PHP ) and three novel genes. The distribution of Alu and LINE1 repetitive elements and the densities of predicted exons were different in each region, and Alu -rich portions contained more exonic sequences than LINE1-rich areas.","subitem_description_type":"Other"},{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"en","subitem_1522300316516":"Oxford University Press"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"eng"}]},"item_1617186783814":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/20.500.12000/46345"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"1340-2838"},{"subitem_1522646500366":"ISSN","subitem_1522646572813":"1756-1663"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"en","subitem_1522650091861":"DNA Research"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-12-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"400","bibliographicPageStart":"387","bibliographicVolumeNumber":"6"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_1522306287251":{"subitem_1522306382014":"DOI","subitem_1522306436033":"https://doi.org/10.1093/dnares/6.6.387"}},{"subitem_1522306287251":{"subitem_1522306382014":"DOI","subitem_1522306436033":"https://doi.org/10.1093/dnares/6.6.387"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"dnares_6_6_387.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2012535/files/dnares_6_6_387.pdf"},"version_id":"965689e3-96ea-43e7-9290-fd8298ba6469"}]},"item_title":"Sequence Analysis of a Total of Three Megabases of DNA in Two Regions of Chromosome 8p","item_type_id":"15","owner":"1","path":["1642838338003","1642838407795"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-06-30"},"publish_date":"2020-06-30","publish_status":"0","recid":"2012535","relation_version_is_last":true,"title":["Sequence Analysis of a Total of Three Megabases of DNA in Two Regions of Chromosome 8p"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-08-03T05:31:15.838583+00:00"}