{"created":"2022-02-02T02:13:45.978511+00:00","id":2012565,"links":{},"metadata":{"_buckets":{"deposit":"762d487f-e3c2-465d-865e-1dd0cc65d92b"},"_deposit":{"id":"2012565","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2012565"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02012565","sets":["1642838163960:1642838338003","1642838403551:1642838407795"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"Characteristic development of the GABA-removal system in the mouse spinal cord","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kim, J.","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kosaka, Y.","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Shimizu-Okabe, C.","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Niizaki, A.","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Takayama, C.","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_1522650717957":"en","subitem_1522651041219":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0"},{"subitem_1522650717957":"en","subitem_1522650727486":"https://creativecommons.org/licenses/by-nc-nd/4.0/","subitem_1522651041219":"https://creativecommons.org/licenses/by-nc-nd/4.0/"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"astrocyte"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"GABA transporter 1 (GAT-1)"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"GABA transporter 3 (GAT-3)"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"GABAergic synapse"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"radial glia"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"vesicular GABA transporter (VGAT)"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"GABA is a predominant inhibitory neurotransmitter in the CNS. Released GABA is removed from the synaptic cleft by two GABA transporters (GATs), GAT-1 and GAT-3, and their dysfunction affects brain functions. The present study aimed to reveal the ontogeny of the GABA-removal system by examining the immunohistochemical localization of GAT-1 and GAT-3 in the embryonic and postnatal mouse cervical spinal cord. In the dorsal horn, GAT-1 was localized within the presynapses of inhibitory axons after embryonic day 15 (E15), a little prior to GABAergic synapse formation. The GAT-1-positive dots increased in density until postnatal day 21 (P21). By contrast, in the ventral horn, GAT-1-positive dots were sparse during development, although many transient GABAergic synapses were formed before birth. GAT-3 was first localized within the radial processes of radial glia in the ventral part on E12 and the dorsal part on E15. The initial localization of the GAT-3 was almost concomitant with the dispersal of GABAergic neurons. GAT-3 continued to be localized within the processes of astrocytes, and increased in expression until P21. These results suggested the following: (1) before synapse formation, GABA may be transported into the processes of radial glia or immature astrocytes by GAT-3. (2) At the transient GABAergic synapses in the ventral horn, GABA may not be reuptaken into the presynapses. (3) In the dorsal horn, GABA may start to be reuptaken by GAT-1 a little prior to synapse formation. (4) After synapse formation, GAT-3 may continue to remove GABA from immature and mature synaptic clefts into the processes of astrocytes. (5) Development of the GABA-removal system may be completed by P21.","subitem_description_type":"Other"},{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"en","subitem_1522300316516":"Elsevier"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"eng"}]},"item_1617186783814":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/20.500.12000/46548"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"0306-4522"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"en","subitem_1522650091861":"Neuroscience"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-03-14","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"142","bibliographicPageStart":"129","bibliographicVolumeNumber":"262"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"AM","subitem_1600292170262":"http://purl.org/coar/version/c_ab4af688f83e57aa"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_1522306287251":{"subitem_1522306382014":"DOI","subitem_1522306436033":"https://doi.org/10.1016/j.neuroscience.2013.12.066"}},{"subitem_1522306287251":{"subitem_1522306382014":"DOI","subitem_1522306436033":"https://doi.org/10.1016/j.neuroscience.2013.12.066"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"FinFigure11rev.tif","mimetype":"image/tiff","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2012565/files/FinFigure11rev.tif"},"version_id":"9fbd6d22-b138-4214-a105-0c64b87c63ea"},{"accessrole":"open_access","filename":"Figure10.tif","mimetype":"image/tiff","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2012565/files/Figure10.tif"},"version_id":"2051bdf3-5199-4474-ad9b-550a1af2ddbc"},{"accessrole":"open_access","filename":"Figure9.tif","mimetype":"image/tiff","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2012565/files/Figure9.tif"},"version_id":"c1c87fbf-22b1-4940-8427-c9557860fd8a"},{"accessrole":"open_access","filename":"Figure8.tif","mimetype":"image/tiff","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2012565/files/Figure8.tif"},"version_id":"9ca8e6a4-ac6e-43e8-8d90-4157b8e953b8"},{"accessrole":"open_access","filename":"Figure7.tif","mimetype":"image/tiff","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2012565/files/Figure7.tif"},"version_id":"c1a6422e-3153-483a-9ec5-f04efd227419"},{"accessrole":"open_access","filename":"Figure6.tif","mimetype":"image/tiff","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2012565/files/Figure6.tif"},"version_id":"86a3d6e9-1001-417e-95aa-cdfc3a653f5a"},{"accessrole":"open_access","filename":"Figure5.tif","mimetype":"image/tiff","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2012565/files/Figure5.tif"},"version_id":"3ec311d0-ad41-4dff-b44f-7e1081df0ce3"},{"accessrole":"open_access","filename":"Figure4.tif","mimetype":"image/tiff","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2012565/files/Figure4.tif"},"version_id":"bd6fb0f3-985f-440a-bc1e-fe7b945fd111"},{"accessrole":"open_access","filename":"Figure3.tif","mimetype":"image/tiff","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2012565/files/Figure3.tif"},"version_id":"2f374c51-6ec8-4832-97e4-39e36e6944d5"},{"accessrole":"open_access","filename":"Figure2.tif","mimetype":"image/tiff","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2012565/files/Figure2.tif"},"version_id":"535038dd-f4ce-44bf-b096-45d52cef5d4a"},{"accessrole":"open_access","filename":"Figure1.tif","mimetype":"image/tiff","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2012565/files/Figure1.tif"},"version_id":"debe2b81-bf58-46a9-bb41-5f4db675881a"},{"accessrole":"open_access","filename":"262.14.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2012565/files/262.14.pdf"},"version_id":"c6bd8436-1cc8-4011-8da2-12e800e046e0"}]},"item_title":"Characteristic development of the GABA-removal system in the mouse spinal cord","item_type_id":"15","owner":"1","path":["1642838338003","1642838407795"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-07-31"},"publish_date":"2020-07-31","publish_status":"0","recid":"2012565","relation_version_is_last":true,"title":["Characteristic development of the GABA-removal system in the mouse spinal cord"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-08-03T05:25:14.791541+00:00"}