{"created":"2022-02-22T03:01:12.510615+00:00","id":2015584,"links":{},"metadata":{"_buckets":{"deposit":"04293e0e-76b3-425e-ba7b-47f8e0bd7777"},"_deposit":{"id":"2015584","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2015584"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02015584","sets":["1642838163960:1642838198944:1642838199408:1642838225283","1642838403551:1642838412624"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"［原著］Repair of Osteochondral Defects in Rat Knees with HumanBone Marrow Mesenchymal Stem Cell","subitem_1551255648112":"ja"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hayashi, Kaori","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kanaya, Fminori","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Fu, Freddy H","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_1522650717957":"ja","subitem_1522651041219":"琉球医学会"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"mesenchymal"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"stem cell"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"osteochondral defect"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"cartilage repair"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"pellet culture"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"transplantation"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Articular cartilage (hyaline cartilage) has only a limited capacity for intrinsic healing. Human mesenchymal stem cells (MSCs) from bone marrow are useful candidate for cell sources in tissue engineering, since MSCs have the capacity to differentiate into various tissues. In this study, the pellets containing hyaline like cartilage differentiated from MSCs were transplanted in rat knee joints in an attempt to repair full-thickness osteochondral defect.  MSCs, which were obtained by isolating from human bone marrow, were centrifuged  and the resultant pellet was cultured in a medium containing various concentration of TGFbeta1  in the presence or absence of fetal bovine serum (FBS) for 2 weeks. As the results, the  pellets cultured under a condition with 10 ng/ml TGF-beta1 and absence of FBS contained  significant hyaline like cartilage that were evaluated histologically. The pellets cultured  under this condition were transplanted into the osteochondral defect in the patellar groove  of athymic rat knee joints. The transplanted pellets were covered with fibrin glue (pellet  group) , whereas the osteochondral defect filled only with fibrin glue was used as a control  (control group). At 4, 8, 16, 24 weeks after the treatment, we performed macroscopic comparison,  histological evaluation (toluidine blue, Mankin's score) and fluorescence in situ  hybridization (FISH) of human X and Y chromosomes. The macroscopic findings of the  pellet group at 24 weeks showed shiny and smooth surface, whereas the control group  showed rough surface and subsided. After the treatment of osteochondral defect with pellets,  the depth of the defect became thinner sequentially but surface layer consisted of hyaline like cartilage histologically. On the other hands, the surface of the defect treated without  pellets was fibrous tissues. The Mankin's score showed no significant differences among weeks,  however, the score of the pellet group at 24 weeks was significantly lower than that of the control  group, suggesting that the histological repair of the  pellet group was better than that of the  control group. At 2 and 8 weeks after the transplantation, human cells were detected in the  transplanted place but were not observed at 16 weeks by FISH. Although we could not explain  the role of the transplanted cells, the full-thickness cartilage defect treated with pellets showed  macroscopically nearly normal, and the surface of the defect was covered with hyaline like cartilage.  These results suggest that the transplantation of pellets containing hyaline like cartilage  differentiated from MSCs may have potential to restore full-thickness osteochondral defect.","subitem_description_type":"Other"},{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"琉球医学会"},{"subitem_1522300295150":"en","subitem_1522300316516":"Ryukyu Medical Association"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"1346-888X"},{"subitem_1522646500366":"ISSN","subitem_1522646572813":"0289-1530"},{"subitem_1522646500366":"NCID","subitem_1522646572813":"AN10369445"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"ja","subitem_1522650091861":"琉球医学会誌 = Ryukyu Medical Journal"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3・4","bibliographicPageEnd":"122","bibliographicPageStart":"115","bibliographicVolumeNumber":"27"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"v27p115.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2015584/files/v27p115.pdf"},"version_id":"b153b275-6d61-4325-8bc7-d52dd664fff1"}]},"item_title":"［原著］Repair of Osteochondral Defects in Rat Knees with HumanBone Marrow Mesenchymal Stem Cell","item_type_id":"15","owner":"1","path":["1642838225283","1642838412624"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2010-02-23"},"publish_date":"2010-02-23","publish_status":"0","recid":"2015584","relation_version_is_last":true,"title":["［原著］Repair of Osteochondral Defects in Rat Knees with HumanBone Marrow Mesenchymal Stem Cell"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-10-31T07:40:37.318904+00:00"}