{"created":"2022-02-22T03:01:16.114607+00:00","id":2015586,"links":{},"metadata":{"_buckets":{"deposit":"65f1c2c3-011d-43c8-9d7d-c928b47e240f"},"_deposit":{"id":"2015586","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2015586"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02015586","sets":["1642838163960:1642838198944:1642838199408:1642838224750","1642838403551:1642838412624"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"［総説］HIV-1感染に対する新規治療法及び感染実験小動物モデルの開発","subitem_1551255648112":"ja"},{"subitem_1551255647225":"Development of a Novel Anti-HIV 1 Therapy and a Small Animal Model for the Testing of Anti-HIV 1 Drugs","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"大隈, 和","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Okuma, kazu","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_1522650717957":"ja","subitem_1522651041219":"琉球医学会"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"HIV-1"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"VSV"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"viral receptor"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"animal model"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"interleukin-4"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Highly active anti-retroviral therapy (HAART) is effective against human immunodeficiency virus type 1 ( HIV-1) infection, but several severe problems such as occurrence of multi-drug resistant (MDR) viruses are emerging after use of the currently available drugs. Thus, development of novel therapeutics with a different mechanism of action is urgently needed. A recombinant vesicular stomatitis virus (VSV) lacking its G gene ($ \\Delta $G) and instead encoding human CD4 and CXCR4 is known to control CXCR4-tropic (X4) HIV-1 infection in culture. To test the value of such a recombiant virus more, we constructed VSV $ \\Delta $ Gs expressing CD4 and CCR5 with or without modified dendritic cellspecific ICAM-3 grabbing nonintegrin (DC-SIGN) from rhesus macaques and examined if these viruses were effective against simian immunodeficiency virus (SIV) infection in culture. Our data demonstrated that the VSV $ \\Delta $ Gs specifically infected and killed SIVinfected cells, resulting in long-term control of SIV infection in vitro. These results indicate that such VSVs have potential for novel anti-HIV-1 therapeutic candidates. In addition, to determine therapeutic value of anti-HIV-1 drug candidates, it is crucial to test them in animal models before clinical trials. Severe combined immunodeficiency (SCID) mice reconstituted with human peripheral blood mononuclear cells (PBMCs),termed hu-PBLSCID mice or humanized mice, have served as such a valuable small animal model. However, there was a limitation on use of this model because X4 HIV-1 does not efficiently infect these mice due to, at least in part, relatively low levels of expression of the CXCR4 coreceptor. To solve this problem, we generated human interleukin (IL)-4-transgenic huPBL-SCID mice that spontaneously synthesized human IL-4 which has been shown to enhance CXCR4 expression and promote X4 HIV-1 infection in vitro and investigated if X4 virus efficiently infected these mice. Our study showed that in vivo synthesis of human IL-4 augmented CXCR4 expression on human CD4+ T cells and led to productive X4 HIV-1 infection, and that the in vivo infection was definitely blocked by CXCR4 antagonist administration. These data indicate that human IL-4-transgenic hu-PBL-SCID mice can be a useful model for the X4 HIV-1 study and the testing of anti-X4 HIV-1 drug candidates.","subitem_description_type":"Other"},{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"琉球医学会"},{"subitem_1522300295150":"en","subitem_1522300316516":"Ryukyu Medical Association"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"jpn"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"1346-888X"},{"subitem_1522646500366":"ISSN","subitem_1522646572813":"0289-1530"},{"subitem_1522646500366":"NCID","subitem_1522646572813":"AN10369445"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"ja","subitem_1522650091861":"琉球医学会誌 = Ryukyu Medical Journal"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1・2","bibliographicPageEnd":"9","bibliographicPageStart":"1","bibliographicVolumeNumber":"27"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"v27p1.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2015586/files/v27p1.pdf"},"version_id":"5eb88b04-6c0c-4514-80e3-748b7ed56920"}]},"item_title":"［総説］HIV-1感染に対する新規治療法及び感染実験小動物モデルの開発","item_type_id":"15","owner":"1","path":["1642838224750","1642838412624"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2010-02-23"},"publish_date":"2010-02-23","publish_status":"0","recid":"2015586","relation_version_is_last":true,"title":["［総説］HIV-1感染に対する新規治療法及び感染実験小動物モデルの開発"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-10-31T07:40:39.407589+00:00"}