{"created":"2022-02-22T03:13:58.609385+00:00","id":2015734,"links":{},"metadata":{"_buckets":{"deposit":"6cf23414-0b35-47e3-a50e-0f6c0b77bb31"},"_deposit":{"id":"2015734","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2015734"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02015734","sets":["1642838163960:1642838198944:1642838233003:1642838234333","1642838403551:1642838412624"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"[原著］Estimation of Sodium- Potassium ATPase Activity of Skeletal Muscle Hemogenate and Washed Particle","subitem_1551255648112":"ja"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takeo, Satoshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Sakanashi, Matao","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Aniya, Yoko","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_1522650717957":"ja","subitem_1522651041219":"琉球医学会"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Sodium-potassium ATPase"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Ouabain"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Red muscle"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Vanadium"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"White muscle"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Applicability of ouabain and vanadium for estimation of Na-K ATPase of crude fractions of skeletal red and white muscles was examined. More than 3 mM ouabain or 300 nM vanadium significantly reduced magnesium-activated ATPase activity of skeletal muscle homogenate and washed particle. Na-K ATPase activity of red muscle homogenate estimated using 1 mM ouabain was about 2-fold higher than that of the washed particle, while the Na-K ATPase activity of white muscle homogenate was about half in comparison with that of the washed particle. This indicates differences in changes of Na-K ATPase activity between skeletal red and white muscles during preparation of washed particle from the homogenate. Na-K ATPase activity of skeletal muscle homogenate estimated using 100 nM vanadium showed a similar activity to that of the washed particle, which was lower than that estimated by 1 mM ouabain. The present results suggest applicability of ouabain as a tool for estimation of Na-K ATPase in skeletal muscle crude fraction.","subitem_description_type":"Other"},{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"琉球大学医学部"},{"subitem_1522300295150":"en","subitem_1522300316516":"Faculty of Medicine, University of the Ryukyus"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"0289-1530"},{"subitem_1522646500366":"NCID","subitem_1522646572813":"AN10027430"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"ja","subitem_1522650091861":"琉球大学医学会雑誌 : 医学部紀要 = Ryukyu medical journal"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1983","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3-4","bibliographicPageEnd":"166","bibliographicPageStart":"158","bibliographicVolumeNumber":"6"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"v6p158.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2015734/files/v6p158.pdf"},"version_id":"1b36df15-656b-4f97-a105-1e21c61e7c7a"}]},"item_title":"[原著］Estimation of Sodium- Potassium ATPase Activity of Skeletal Muscle Hemogenate and Washed Particle","item_type_id":"15","owner":"1","path":["1642838234333","1642838412624"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2010-06-30"},"publish_date":"2010-06-30","publish_status":"0","recid":"2015734","relation_version_is_last":true,"title":["[原著］Estimation of Sodium- Potassium ATPase Activity of Skeletal Muscle Hemogenate and Washed Particle"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-10-31T07:43:29.110702+00:00"}