@article{oai:u-ryukyu.repo.nii.ac.jp:02015892,
 author = {Kono, Shinzo and Sunagawa, Hajime},
 issue = {3},
 journal = {琉球医学会誌 = Ryukyu Medical Journal},
 month = {},
 note = {A specific and highly sensitive radioimmunoassay of estradiol-17$ \beta $ (E_2-17$ \beta $) was developed. Ether was used to extract E_2-I7 from 0.5ml plasma samples and the extract was then purified by Sephadex LH-20 column chromatography and measured using an anti-serum to E_2-6-BSA and I^<125>-labelled E_2-17$ \beta $ Sensitivity of the method was 2.5pg/ml. The coefficient of variation in accuracy and precision of the method was under 15%. The unconjugated E_2-17$ \beta $ concentration in sera of 21 postmenopausal women ranged from 2.5 to 14.1 pg/ml. In serum samples over 10 pg/ml investigated by the direct method, E_2-17$ \beta $ values determined by the present method correlated well with the direct method (r=0.91,pく0.001, N=87). In samples under 10 pg/ml determined by the direct method, E_2-17$ \beta $ value ranged from 2.5 to 23.5 pg/ml by the present method. The present method might be useful for more accurate evaluations of ovarian functions., 論文},
 pages = {219--225},
 title = {[原著］Specific and Sensitive Estradiol-17 $ \beta $ Radioimmunoassay},
 volume = {12},
 year = {1992}
}