{"created":"2022-02-22T03:32:46.774568+00:00","id":2015939,"links":{},"metadata":{"_buckets":{"deposit":"f828939e-33a8-44f3-977b-56bf1d626cd9"},"_deposit":{"id":"2015939","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2015939"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02015939","sets":["1642838163960:1642838198944:1642838199408:1642838206792","1642838403551:1642838412624"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"[原著］Clinical evaluation of rapid detection of Mycobacterium tuberculosis using a polymerase chain reaction method","subitem_1551255648112":"ja"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Zhang Kou-Xing","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Higa, Futoshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Koide, Michio","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kusano, Nobuchika","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Saito, Atsushi","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_1522650717957":"ja","subitem_1522651041219":"琉球医学会"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Polymerase chain reaction"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Mycobacterium tuberculosis"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"clinical diagnosis"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"The polymerase chain reaction (PCR) for identification of Mycobacterium tuberculosis in uncultured clinical specimens was evaluated prospectively. Two oligonuleotide primers were derived from the nucleotide sequence of IS 6110, which amplified a 123-bp sequence found in members of the M. tuberculosis complex. The detection limit of M. tuberculosis DNA was 10 fg by gel electrophoresis and ethidium bromide staining after a forty cycle amplification. A total of 100 clinical specimens (from 70 patients) were investigated using the PCR method. Twenty-one samples were found to be positive and seventy-nine were negative. Compared with the conventional culture method, sensitivity and specificity of the PCR method's were 94.0% and 94.1%, respectively. Cases with PCR repeatedly positive revealed definite tuberculosis. Nineteen of the positive samples were from active culture positive tuberculosis cases, and one from a patient with inproved condition after anti-tuberculosis treatment. Seventy-nine negative samples were from various respiratory diseases, including old inactive tuberculosis or active tuberculosis which had already been treated with anti-tuberculosis drugs. In this study, PCR results could be obtained within two days. The PCR method was found to be rapid, sensitive, and reliable test for clinical consideration.","subitem_description_type":"Other"},{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"琉球医学会"},{"subitem_1522300295150":"en","subitem_1522300316516":"Ryukyu Medical Association"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"1346888X"},{"subitem_1522646500366":"ISSN","subitem_1522646572813":"0289-1530"},{"subitem_1522646500366":"NCID","subitem_1522646572813":"AN10369445"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"ja","subitem_1522650091861":"琉球医学会誌 = Ryukyu Medical Journal"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"131","bibliographicPageStart":"127","bibliographicVolumeNumber":"15"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"v15p127.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2015939/files/v15p127.pdf"},"version_id":"ab99586e-0797-4051-9e27-628ecb12d72f"}]},"item_title":"[原著］Clinical evaluation of rapid detection of Mycobacterium tuberculosis using a polymerase chain reaction method","item_type_id":"15","owner":"1","path":["1642838206792","1642838412624"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2010-07-02"},"publish_date":"2010-07-02","publish_status":"0","recid":"2015939","relation_version_is_last":true,"title":["[原著］Clinical evaluation of rapid detection of Mycobacterium tuberculosis using a polymerase chain reaction method"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-10-31T07:47:18.068316+00:00"}