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  1. 学術雑誌論文
  2. 琉球医学会
  3. 琉球医学会誌
  4. 19巻4号
  1. 部局別インデックス
  2. その他

[原著]Effect of Bcl-2 expression on morphological alteration of hepatic mitochondria in liver regeneration after a partial hepatectomy : An immunohistochemical and ultrastructural study

http://hdl.handle.net/20.500.12000/0002016044
http://hdl.handle.net/20.500.12000/0002016044
9d2d76b6-3232-4956-8791-7f76c9ebcb5f
名前 / ファイル ライセンス アクション
v19p203.pdf v19p203.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2010-07-02
タイトル
タイトル [原著]Effect of Bcl-2 expression on morphological alteration of hepatic mitochondria in liver regeneration after a partial hepatectomy : An immunohistochemical and ultrastructural study
言語 ja
作成者 Mamadi, Toure

× Mamadi, Toure

en Mamadi, Toure

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Shiraishi, Masayuki

× Shiraishi, Masayuki

en Shiraishi, Masayuki

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Taira, Kaoru

× Taira, Kaoru

en Taira, Kaoru

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Muto, Yoshihiro

× Muto, Yoshihiro

en Muto, Yoshihiro

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
言語 ja
権利情報 琉球医学会
主題
言語 en
主題Scheme Other
主題 Bcl-2
主題
言語 en
主題Scheme Other
主題 mitochondria
主題
言語 en
主題Scheme Other
主題 liver regeneration
主題
言語 en
主題Scheme Other
主題 electron-microscopy
内容記述
内容記述タイプ Other
内容記述 We investigated the effects of Bcl-2 0verexpression on the morphological changes of the mitochondria (MC) in the regenerating rat liver after a 70% partial hepatectomy (PH). Bcl-2 or control marker (LacZ) gene transfection to the regenerating liver was performed immediately after PH by a systemical injection of 1X10^9 pfu/body of recombinant adenovirus, which encodes either human Bcl-2 protein (ACalbBcl2pLpA) in group 1 (n=39) or E.Coli $ \beta $-galactosidase (AxCALacZ) in group 2 (n=39). Ingroup3 (n=13), 1ml of normal saline was injected instead of the recombinant adenovirus. The rats were allowed to survive until the scheduled sacrifice at 0.5h, 2h, 4h, 12h, 24h, 2d, 3d, 5d, 7d, 9d, 11d, 14d, and 21d after PH (3 rats each). In the immunohistochemical analysis, the Bcl-2 protein expression showed a peak intensity at 12 to 72 hrs after PH in group 1, whereas it was delayed until 5 days after PH in groups 2 and 3 vMth a rather mild intensity, followed by a gradual decrease in all three groups. At its peak intensity, the Bel-2 protein expression was recognized over a wide range of the parenchymal area as well as in the penportal area. The $ \beta $-galactosidase activities in X-gal staining, showed apeak intensity at 48 hrs after PH followed by a gradual decrease until 21 days after PH in group 2. In the hepatocyte ultrastructure, MC shape was well preserved in group 1 until 7 days after PH with number of regular shaped mitochondria, well preserved cristae and dense matrix (RM) being $58 \pm 22$% at 12hrs and $60 \pm 21$% at 24 hrs, compared to groups 2 ($36 \pm 23$% at 12hrs and $40 \pm 15$% at 24hrs, p≦0.015 and ≦0.004 respectively) and 3 ($29 \pm 11$% at 12hrs and $35 \pm 12$% at 24 hrs, p≦0.0004 and ≦0.0015 respectively). In conclusion, Bcl-2 0verexpression in the regenerating liver was important in preserving mitochondrial shape in the early stage of liver regeneration, thus contributing to an accelerated liver regeneration.
内容記述
内容記述タイプ Other
内容記述 論文
出版者
言語 ja
出版者 琉球医学会
出版者
言語 en
出版者 Ryukyu Medical Association
言語
言語 eng
資源タイプ
資源タイプ journal article
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 1346888X
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0289-1530
収録物識別子
収録物識別子タイプ NCID
収録物識別子 AN10369445
収録物名
言語 ja
収録物名 琉球医学会誌 = Ryukyu Medical Journal
書誌情報
巻 19, 号 4, p. 203-209, 発行日 1999
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