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[原著］Effect of Bcl-2 expression on morphological alteration of hepatic mitochondria in liver regeneration after a partial hepatectomy : An immunohistochemical and ultrastructural study
http://hdl.handle.net/20.500.12000/0002016044
http://hdl.handle.net/20.500.12000/00020160449d2d76b6-3232-4956-8791-7f76c9ebcb5f
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
v19p203.pdf
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| Item type | デフォルトアイテムタイプ（フル）(1) | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2010-07-02 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | [原著］Effect of Bcl-2 expression on morphological alteration of hepatic mitochondria in liver regeneration after a partial hepatectomy : An immunohistochemical and ultrastructural study | |||||||||||||
| 言語 | ja | |||||||||||||
| 作成者 |
Mamadi, Toure
× Mamadi, Toure
× Shiraishi, Masayuki
× Taira, Kaoru
× Muto, Yoshihiro
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| アクセス権 | ||||||||||||||
| アクセス権 | open access | |||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||
| 権利情報 | ||||||||||||||
| 言語 | ja | |||||||||||||
| 権利情報 | 琉球医学会 | |||||||||||||
| 主題 | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Bcl-2 | |||||||||||||
| 主題 | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | mitochondria | |||||||||||||
| 主題 | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | liver regeneration | |||||||||||||
| 主題 | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | electron-microscopy | |||||||||||||
| 内容記述 | ||||||||||||||
| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | We investigated the effects of Bcl-2 0verexpression on the morphological changes of the mitochondria (MC) in the regenerating rat liver after a 70% partial hepatectomy (PH). Bcl-2 or control marker (LacZ) gene transfection to the regenerating liver was performed immediately after PH by a systemical injection of 1X10^9 pfu/body of recombinant adenovirus, which encodes either human Bcl-2 protein (ACalbBcl2pLpA) in group 1 (n=39) or E.Coli $ \beta $-galactosidase (AxCALacZ) in group 2 (n=39). Ingroup3 (n=13), 1ml of normal saline was injected instead of the recombinant adenovirus. The rats were allowed to survive until the scheduled sacrifice at 0.5h, 2h, 4h, 12h, 24h, 2d, 3d, 5d, 7d, 9d, 11d, 14d, and 21d after PH (3 rats each). In the immunohistochemical analysis, the Bcl-2 protein expression showed a peak intensity at 12 to 72 hrs after PH in group 1, whereas it was delayed until 5 days after PH in groups 2 and 3 vMth a rather mild intensity, followed by a gradual decrease in all three groups. At its peak intensity, the Bel-2 protein expression was recognized over a wide range of the parenchymal area as well as in the penportal area. The $ \beta $-galactosidase activities in X-gal staining, showed apeak intensity at 48 hrs after PH followed by a gradual decrease until 21 days after PH in group 2. In the hepatocyte ultrastructure, MC shape was well preserved in group 1 until 7 days after PH with number of regular shaped mitochondria, well preserved cristae and dense matrix (RM) being $58 \pm 22$% at 12hrs and $60 \pm 21$% at 24 hrs, compared to groups 2 ($36 \pm 23$% at 12hrs and $40 \pm 15$% at 24hrs, p≦0.015 and ≦0.004 respectively) and 3 ($29 \pm 11$% at 12hrs and $35 \pm 12$% at 24 hrs, p≦0.0004 and ≦0.0015 respectively). In conclusion, Bcl-2 0verexpression in the regenerating liver was important in preserving mitochondrial shape in the early stage of liver regeneration, thus contributing to an accelerated liver regeneration. | |||||||||||||
| 内容記述 | ||||||||||||||
| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | 論文 | |||||||||||||
| 出版者 | ||||||||||||||
| 出版者 | 琉球医学会 | |||||||||||||
| 言語 | ja | |||||||||||||
| 出版者 | ||||||||||||||
| 出版者 | Ryukyu Medical Association | |||||||||||||
| 言語 | en | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| 出版タイプ | ||||||||||||||
| 出版タイプ | VoR | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
| 収録物識別子 | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 1346888X | |||||||||||||
| 収録物識別子 | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 0289-1530 | |||||||||||||
| 収録物識別子 | ||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||
| 収録物識別子 | AN10369445 | |||||||||||||
| 収録物名 | ||||||||||||||
| 収録物名 | 琉球医学会誌 = Ryukyu Medical Journal | |||||||||||||
| 言語 | ja | |||||||||||||
| 書誌情報 |
巻 19, 号 4, p. 203-209, 発行日 1999 |
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