{"created":"2022-02-22T04:11:57.406341+00:00","id":2016044,"links":{},"metadata":{"_buckets":{"deposit":"b480eafb-52cf-4262-955c-a49c03900926"},"_deposit":{"id":"2016044","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2016044"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02016044","sets":["1642838163960:1642838198944:1642838199408:1642838214651","1642838403551:1642838412624"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"[原著］Effect of Bcl-2 expression on morphological alteration of hepatic mitochondria in liver regeneration after a partial hepatectomy : An immunohistochemical and ultrastructural study","subitem_1551255648112":"ja"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mamadi, Toure","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Shiraishi, Masayuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Taira, Kaoru","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Muto, Yoshihiro","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_1522650717957":"ja","subitem_1522651041219":"琉球医学会"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Bcl-2"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"mitochondria"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"liver regeneration"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"electron-microscopy"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"We investigated the effects of Bcl-2 0verexpression on the morphological changes of the mitochondria (MC) in the regenerating rat liver after a 70% partial hepatectomy (PH). Bcl-2 or control marker (LacZ) gene transfection to the regenerating liver was performed immediately after PH by a systemical injection of 1X10^9 pfu/body of recombinant adenovirus, which encodes either human Bcl-2 protein (ACalbBcl2pLpA) in group 1 (n=39) or E.Coli $ \\beta $-galactosidase (AxCALacZ) in group 2 (n=39). Ingroup3 (n=13), 1ml of normal saline was injected instead of the recombinant adenovirus. The rats were allowed to survive until the scheduled sacrifice at 0.5h, 2h, 4h, 12h, 24h, 2d, 3d, 5d, 7d, 9d, 11d, 14d, and 21d after PH (3 rats each). In the immunohistochemical analysis, the Bcl-2 protein expression showed a peak intensity at 12 to 72 hrs after PH in group 1, whereas it was delayed until 5 days after PH in groups 2 and 3 vMth a rather mild intensity, followed by a gradual decrease in all three groups. At its peak intensity, the Bel-2 protein expression was recognized over a wide range of the parenchymal area as well as in the penportal area. The $ \\beta $-galactosidase activities in X-gal staining, showed apeak intensity at 48 hrs after PH followed by a gradual decrease until 21 days after PH in group 2. In the hepatocyte ultrastructure, MC shape was well preserved in group 1 until 7 days after PH with number of regular shaped mitochondria, well preserved cristae and dense matrix (RM) being $58 \\pm 22$% at 12hrs and $60 \\pm 21$% at 24 hrs, compared to groups 2 ($36 \\pm 23$% at 12hrs and $40 \\pm 15$% at 24hrs, p≦0.015 and ≦0.004 respectively) and 3 ($29 \\pm 11$% at 12hrs and $35 \\pm 12$% at 24 hrs, p≦0.0004 and ≦0.0015 respectively). In conclusion, Bcl-2 0verexpression in the regenerating liver was important in preserving mitochondrial shape in the early stage of liver regeneration, thus contributing to an accelerated liver regeneration.","subitem_description_type":"Other"},{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"琉球医学会"},{"subitem_1522300295150":"en","subitem_1522300316516":"Ryukyu Medical Association"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"1346888X"},{"subitem_1522646500366":"ISSN","subitem_1522646572813":"0289-1530"},{"subitem_1522646500366":"NCID","subitem_1522646572813":"AN10369445"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"ja","subitem_1522650091861":"琉球医学会誌 = Ryukyu Medical Journal"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"209","bibliographicPageStart":"203","bibliographicVolumeNumber":"19"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"v19p203.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2016044/files/v19p203.pdf"},"version_id":"d37cbcee-33c8-4ec5-83fb-95051060d045"}]},"item_title":"[原著］Effect of Bcl-2 expression on morphological alteration of hepatic mitochondria in liver regeneration after a partial hepatectomy : An immunohistochemical and ultrastructural study","item_type_id":"15","owner":"1","path":["1642838214651","1642838412624"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2010-07-02"},"publish_date":"2010-07-02","publish_status":"0","recid":"2016044","relation_version_is_last":true,"title":["[原著］Effect of Bcl-2 expression on morphological alteration of hepatic mitochondria in liver regeneration after a partial hepatectomy : An immunohistochemical and ultrastructural study"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-10-31T07:49:14.262734+00:00"}