@article{oai:u-ryukyu.repo.nii.ac.jp:02016083,
 author = {Shiraishi, Masayuki and Nozato, Eiji and Okuhama, Yukihiro and Tomori, Hirofumi and Higa, Takao and Taira, Kaoru and Muto, Yoshihiro},
 issue = {3},
 journal = {琉球医学会誌 = Ryukyu Medical Journal},
 month = {},
 note = {Various models of hepatic ischemia/reperfusion (I/R) injury were tested, using both rats (groups 1, 2 and 3, each n=10) and pigs (groups4 and5, each n=5). To create cold ischemia for 1 hr, an orthotopic liver transplantation (OLT) was performed in groups 1 and 4 using the cuff technique to reconstruct the portal vein and infra-hepatic vein in group 1, and using the veno-venous bypass during a non-hepatic phase in group 4. In groups 3 and 5, in-situ perfusion of the liver was performed without taking out the liver under a portosystemic shunt, which was achieved by a splenic transposition (created 4 weeks prior to the surgery) in group 3, and by a pump controlled veno-venous bypass in group 5. In group 2, a simple clamp of the portal triads was applied, without flushing out the blood from the liver. The survival rate at 1 week after surgery was 90% (9/10), 70% (7/10), 100% (10/10), 60% (3/5), 100% (5/5) in groups 1 to 5, respectively. Regarding the macroscopic findings of the liver 1 week after ischemia, lobar necrosis (1 case) and patchy necrosis (3 case) were characteristically found in group 1, but not in either groups 2 or 3. The serum hepatic enzyme levels showed a large deviation from case to case in groups 1, 2, and 4, in response to the same amount of cold ischemia. However, these values were relatively uniform in groups 3 and 5, in which in-situ per fusion was performed. In conclusion, the above described in-situ perfusion model of the liver is thus considered to provide a stable in-vivo model for studying hepatic I/R injury, in both rats and pigs., 論文},
 pages = {73--77},
 title = {[原著］In-situ perfusion of the liver as a stable model of cold ischemia in orthotopic liver transplantation},
 volume = {18},
 year = {1998}
}