{"created":"2022-02-22T04:14:25.029061+00:00","id":2016118,"links":{},"metadata":{"_buckets":{"deposit":"69085415-c662-493d-8943-2ccc0f94661f"},"_deposit":{"id":"2016118","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2016118"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02016118","sets":["1642838163960:1642838198944:1642838199408:1642838209529","1642838403551:1642838412624"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"[原著］Graft-directed gene transfection in a rat model of liver transplantation","subitem_1551255648112":"ja"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shiraishi, Masayuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hiroyasu, Shungo","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Tomori, Hirohumi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ma, Shao-ping","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Makino, Yoshihiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kusano, Toshiomi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Muto, Yoshihiro","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_1522650717957":"ja","subitem_1522651041219":"琉球医学会"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"gene transfer"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"adenovirus"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"retrovirus"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"liver transplantation"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"In this model of liver transplantation, liver grafts were transfected with the El-deleted, replication-defective adenovirus vectors encoding the LacZ gene driven by the human CMV promoter, or the replication-defective retrovirus vectors encoding the human IL-7 and neomycin phosphotransferase genes. Liver grafts were perfused ex-vivo with either of these two vectors during cold preservation time. In adenovirus-mediated gene transfer, positive staining of the hepatocyte was recognized predominantly around the portal triads at post-operative day 2. Positive staining was observed until 14 days after transfection. In a retrovirus-mediated gene transfection, gene transfection was tested both in a whole-size and a reduced-size graft implantation of the liver because the retrovirus vector requires a replication of the target cells for gene transfection. In conclusion, adenovirus-mediated gene transfection to the liver graft during cold preservation time resulted in an effective expression of the transfected sequence. On the other hand, retrovirus vector required the reduction of the liver graft for a successful gene transfection.","subitem_description_type":"Other"},{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"琉球医学会"},{"subitem_1522300295150":"en","subitem_1522300316516":"Ryukyu Medical Association"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"1346888X"},{"subitem_1522646500366":"ISSN","subitem_1522646572813":"0289-1530"},{"subitem_1522646500366":"NCID","subitem_1522646572813":"AN10369445"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"ja","subitem_1522650091861":"琉球医学会誌 = Ryukyu Medical Journal"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1997","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"24","bibliographicPageStart":"21","bibliographicVolumeNumber":"17"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"v17p21.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2016118/files/v17p21.pdf"},"version_id":"08e7386b-9262-4323-b52b-afdd209d1828"}]},"item_title":"[原著］Graft-directed gene transfection in a rat model of liver transplantation","item_type_id":"15","owner":"1","path":["1642838209529","1642838412624"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2010-07-02"},"publish_date":"2010-07-02","publish_status":"0","recid":"2016118","relation_version_is_last":true,"title":["[原著］Graft-directed gene transfection in a rat model of liver transplantation"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-10-31T07:50:46.112120+00:00"}