{"created":"2022-02-22T04:15:04.888995+00:00","id":2016140,"links":{},"metadata":{"_buckets":{"deposit":"b9cf018c-e3e0-45ee-87f4-01f8dcad7674"},"_deposit":{"id":"2016140","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2016140"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02016140","sets":["1642838163960:1642838198944:1642838199408:1642838216134","1642838403551:1642838412624"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"[原著］An improved method for the detection of IgE FE-3 by ELISA using monoclonal anti-IgE FE-3 antibody","subitem_1551255648112":"ja"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nakasone,Toshiyuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hanashiro, Kazuhiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nakamura, Mariko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Sunakawa, Hajime","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kosugi, Tadayoshi","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_1522650717957":"ja","subitem_1522651041219":"琉球医学会"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"monoclonal antibody against rat IgE"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"rat IgE"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"DNP-specific rat IgE"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"IgE-capture ELISA"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"A hybridoma producing monoclonal antibody against monoclonal DNP-specific rat IgE (IgE FE-3) was generated by fusion of P3-X63-Ag8-Ul (P3U1) mouse myeloma and spleen cells from BALB/c mice immunized with IgE FE-3. Five hybridoma clones were obtained. Three clones of them had a high reactivity against IgE FE-3 and a low reactivity against the L-chain of IgE FE-3 on ELISA analysis for the first screening of the hybridoma. The binding of monoclonal antibodies produced from clone BB-9 to IgE FE-3 revealed the strongest inhibition among three monoclonal antibodies on pretreatment with polyclonal rabbit anti-IgE FE-3 antibodies. It was clarified that monoclonal antibody BB-9 reacted specifically with the heavy chain of IgE FE-3 by Western blotting analysis. Furthermore, the reactivity of the monoclonal antibody BB-9 to IgE FE-3 was higher than that of MARE-1, which is a monoclonal antibody against rat myeloma IgE. The monoclonal antibody BB-9 was therefore employed for IgE-capture ELISA to determine the concentration of IgE FE-3. No significant alterations of color development occurred when igE FE-3 was concomitantly incubated with large excesses of IgG antibodies and rat myeloma IgE. Our data indicated that IgE-capture ELISA employing the monoclonal antibody BB-9 had a higher sensitivity for the determination of IgE FE-3 than did that using polyclonal rabbit anti-IgE FE-3 antibodies.","subitem_description_type":"Other"},{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"琉球医学会"},{"subitem_1522300295150":"en","subitem_1522300316516":"Ryukyu Medical Association"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"1346888X"},{"subitem_1522646500366":"ISSN","subitem_1522646572813":"0289-1530"},{"subitem_1522646500366":"NCID","subitem_1522646572813":"AN10369445"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"ja","subitem_1522650091861":"琉球医学会誌 = Ryukyu Medical Journal"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2001","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"135","bibliographicPageStart":"129","bibliographicVolumeNumber":"20"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"v20p129.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2016140/files/v20p129.pdf"},"version_id":"f39243dd-994c-4a63-878b-1873c26bc324"}]},"item_title":"[原著］An improved method for the detection of IgE FE-3 by ELISA using monoclonal anti-IgE FE-3 antibody","item_type_id":"15","owner":"1","path":["1642838216134","1642838412624"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2010-07-02"},"publish_date":"2010-07-02","publish_status":"0","recid":"2016140","relation_version_is_last":true,"title":["[原著］An improved method for the detection of IgE FE-3 by ELISA using monoclonal anti-IgE FE-3 antibody"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-10-31T07:51:11.394117+00:00"}