{"created":"2022-02-22T04:15:43.231651+00:00","id":2016162,"links":{},"metadata":{"_buckets":{"deposit":"88f2e8c5-8538-4df1-a07e-2d6991761ddf"},"_deposit":{"id":"2016162","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2016162"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02016162","sets":["1642838163960:1642838198944:1642838199408:1642838220226","1642838403551:1642838412624"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"[原著］A preliminary study on the effect of myeloperoxidase on etoposide-induced HPRT mutants in HL-60 cells","subitem_1551255648112":"ja"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Zheng, Kui-Cheng","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_1522650717957":"ja","subitem_1522651041219":"琉球医学会"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"etoposide"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"myeloperoxidase"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"hypoxanthine phosphoribosyl transferase gene"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"HL-60 cells"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"mutants"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Topoisomerase II (Topo II) -directed antitumor drug, etoposide (VP-16) -related leukemia (T-cell acute myelogenous leukemia, t-AML) occurs in about 10% of drug-treated patients. Understanding the mechanisms underlying leukemia induced by VP-16 is important for the development of safer drugs against leukemia. It has been postulated that redox cycling of VP-16 initiated by myeloperoxidase (MPO) produces reactive oxygen species responsible for the genotoxicity of this agent in myeloid precursors, leading to chromosomal translocations responsible for t-AML. To test this hypothesis, MPO activity of HL-60 cells were down-regulated by treating the cells with succinyl acetone (SA). The genotoxicity of VP-16 in these cells was then determined by analyzing the frequencies of mutations in the hypoxanthine phosphoribosyl transferase (HPRT) gene and compared with that in cells without prior SA-treatment. The results showed that the VP-16-induced HPRT mutant frequency in cells whose MPO activity was inhibited by prior SA treatment was significantly decreased by 78.8%, as compared with cells without prior SA-treatment. These results support our hypothesis that VP-16 induces genotoxicity and carcinogenicity through MPOmediated oxidative activation of VP-16.","subitem_description_type":"Other"},{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"琉球医学会"},{"subitem_1522300295150":"en","subitem_1522300316516":"Ryukyu Medical Association"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"1346888X"},{"subitem_1522646500366":"ISSN","subitem_1522646572813":"0289-1530"},{"subitem_1522646500366":"NCID","subitem_1522646572813":"AN10369445"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"ja","subitem_1522650091861":"琉球医学会誌 = Ryukyu Medical Journal"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueNumber":"3","bibliographicPageEnd":"91","bibliographicPageStart":"85","bibliographicVolumeNumber":"23"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"v23p85.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2016162/files/v23p85.pdf"},"version_id":"ef5cab8c-9c29-40f8-9c30-7c42ce9a0a83"}]},"item_title":"[原著］A preliminary study on the effect of myeloperoxidase on etoposide-induced HPRT mutants in HL-60 cells","item_type_id":"15","owner":"1","path":["1642838220226","1642838412624"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2010-07-02"},"publish_date":"2010-07-02","publish_status":"0","recid":"2016162","relation_version_is_last":true,"title":["[原著］A preliminary study on the effect of myeloperoxidase on etoposide-induced HPRT mutants in HL-60 cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-02-22T04:15:46.468974+00:00"}