{"created":"2022-02-22T04:42:48.092527+00:00","id":2016207,"links":{},"metadata":{"_buckets":{"deposit":"57413811-3182-4969-89f2-c0a65c3833ae"},"_deposit":{"id":"2016207","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2016207"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02016207","sets":["1642838163960:1642838198944:1642838199408:1642838215118","1642838403551:1642838412624"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"[原著］Bile endothelin-1 as a tool for diagnosig ischemia-reperfusion injury","subitem_title_language":"ja"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Miyaguni, Takao","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Shiraishi, Masayuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Tomori, Hirofumi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Okuhama, Yukihiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Higa, Takao","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Muto, Yoshihiro","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"琉球医学会","subitem_rights_language":"ja"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"ischemia-reperfusion injury","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"endothelin-1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"liver","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"We investigated whether the extent of graft ischemia reperfusion (I/R) injury can be evaluated by assessing the level of bile and serum endothelin-1 (ET-1). In groups 1 to 3 (n=5 in each group), the livers of male Wistar rats were surgically isolated from the surrounding attachments and then perfused in-situ with heparinized cold saline (4℃) under a port-systemic shunt. The livers were then reperfused after a pre-determined cold ischemia time (0, 30, and 60 min in groups 1, 2, and 3, respectively). The ET-1 levels in either the bile or the serum obtained from the suprahepatic vena cava (SHVC) and abdominal aorta, were assessed from -1 hour to +72 hours after reperfusion in each group. In group 3, the ET-1 level at the SHVC increased to $19.9 \\pm 2.88$ and $19.43 \\pm 3.14$ pg/ml at 1.5 and 2 hr after reperfusion, respectively, which were significantly higher than those of group 1 ($13.6 \\pm 2.73$, $13.05 0.58\\pm $, p<0.05) and 2 ($11.67 \\pm 2.21$, $12.2 \\pm 2.22$, p<0.05). The bile ET-1 concentration in group 3 decreased at 1 hr after reperfusion ($14.22 \\pm 6.26$ pg/ml), but increased in group 1 ($108.1 \\pm 49.3$). The bile ET-1 concentration did not recover to its pre-ischemic level until 72 hr after reperfusion in group 3, but showed no apparent decrease throughout the experiment in groups 1 and 2. In immunohistochemistry, ET-1 expression on the central and portal vein was stronger in group 3 than in group 1, whereas the expression on the bile duct was weaker in group 3 than that in group 1. In conclusion, ET-1 was directly released from the damaged endothelial cells into the SHVC depending on the extent of the I/R injury, whereas the ET-1 excretion into the bile was inversely suppressed depending on the amount of I/R injury. These changes of ET-1 excretion might thus be useful in diagnosing hepatic I/R injury.","subitem_description_type":"Other"},{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"琉球医学会","subitem_publisher_language":"ja"},{"subitem_publisher":"Ryukyu Medical Association","subitem_publisher_language":"en"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"1346888X","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"0289-1530","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AN10369445","subitem_source_identifier_type":"NCID"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_source_title":"琉球医学会誌 = Ryukyu Medical Journal","subitem_source_title_language":"ja"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueNumber":"1","bibliographicPageEnd":"6","bibliographicPageStart":"1","bibliographicVolumeNumber":"20"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"v20p1.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2016207/files/v20p1.pdf"},"version_id":"da54d939-455e-4bf0-b65a-1323cdd220e8"}]},"item_title":"[原著］Bile endothelin-1 as a tool for diagnosig ischemia-reperfusion injury","item_type_id":"15","owner":"1","path":["1642838215118","1642838412624"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2010-07-02"},"publish_date":"2010-07-02","publish_status":"0","recid":"2016207","relation_version_is_last":true,"title":["[原著］Bile endothelin-1 as a tool for diagnosig ischemia-reperfusion injury"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-02-22T04:42:51.583287+00:00"}