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  1. 学術雑誌論文
  2. 琉球医学会
  3. 琉球大学保健学医学雑誌
  4. 1巻4号
  1. 部局別インデックス
  2. その他

[原著]Deoxyribonuclease Iによる核酸初期分解生成物とb-form核酸との比較

http://hdl.handle.net/20.500.12000/0002016345
http://hdl.handle.net/20.500.12000/0002016345
408fb410-a511-4a75-873e-9a9695fae5ef
名前 / ファイル ライセンス アクション
v1p296.pdf v1p296.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2014-07-18
タイトル
タイトル [原著]Deoxyribonuclease Iによる核酸初期分解生成物とb-form核酸との比較
言語 ja
作成者 井上, 文英

× 井上, 文英

ja 井上, 文英

中田, 福市

× 中田, 福市

ja 中田, 福市

Inoue, Fumihide

× Inoue, Fumihide

en Inoue, Fumihide

Nakada, Fukuichi

× Nakada, Fukuichi

en Nakada, Fukuichi

アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
言語 ja
権利情報 琉球医学会
内容記述
内容記述タイプ Other
内容記述 The purpose of this paper is to elucidate the hydrolytic processes and the physicochemical properties of the initial hydrolysate of thymus DNA by DNase I (EC-3.1.4.5) in the presence of a very low concentration of Mg^<2+>. By examining the increasing curve on the acid soluble material and hyperchromicity, the hydrolytic process of DNA was limited by DNase I under very low Mg^<2+>concentration. The partially hydrolyzed product (ph-DNA) did not show any spectral changes by the disruption of hydrogen bonds involving the reactive amino acid. Therefore, ph-DNA is considered to still retain a double helical structure. ph-DNA was obtained from the reaction mixture under hydrolytic condition and when more Mg^<2+> was added to the reaction mixture, the b-form DNA was produced. It may be concluded that the ph-DNA is the initial degradation product of DNA obtainable by the action of DNase I in the presence of trace Mg^<2+>.
内容記述タイプ Other
内容記述 論文
出版者
言語 ja
出版者 琉球大学保健学部
言語
言語 jpn
資源タイプ
資源タイプ journal article
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0285-9270
収録物識別子タイプ NCID
収録物識別子 AN00250763
収録物名
言語 ja
収録物名 琉球大学保健学医学雑誌=Ryukyu University Journal of Health Sciences and Medicine
書誌情報
巻 1, 号 4, p. 296-300
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