{"created":"2022-02-22T04:55:21.043745+00:00","id":2016345,"links":{},"metadata":{"_buckets":{"deposit":"3958c185-911a-41cb-965c-ad04527f37af"},"_deposit":{"id":"2016345","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2016345"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02016345","sets":["1642838163960:1642838198944:1642838242961:1642838245041","1642838403551:1642838412624"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"[原著]Deoxyribonuclease Iによる核酸初期分解生成物とb-form核酸との比較","subitem_1551255648112":"ja"},{"subitem_1551255647225":"Comparison of a b-form nucleic acid with an initial degradation product produced by deoxyribonuclease I","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"井上, 文英","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"中田, 福市","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Inoue, Fumihide","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nakada, Fukuichi","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_1522650717957":"ja","subitem_1522651041219":"琉球医学会"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"The purpose of this paper is to elucidate the hydrolytic processes and the physicochemical properties of the initial hydrolysate of thymus DNA by DNase I (EC-3.1.4.5) in the presence of a very low concentration of Mg^<2+>. By examining the increasing curve on the acid soluble material and hyperchromicity, the hydrolytic process of DNA was limited by DNase I under very low Mg^<2+>concentration. The partially hydrolyzed product (ph-DNA) did not show any spectral changes by the disruption of hydrogen bonds involving the reactive amino acid. Therefore, ph-DNA is considered to still retain a double helical structure. ph-DNA was obtained from the reaction mixture under hydrolytic condition and when more Mg^<2+> was added to the reaction mixture, the b-form DNA was produced. It may be concluded that the ph-DNA is the initial degradation product of DNA obtainable by the action of DNase I in the presence of trace Mg^<2+>.","subitem_description_type":"Other"},{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"琉球大学保健学部"},{"subitem_1522300295150":"en","subitem_1522300316516":"College of Health Sciences, University of the Ryukyus"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"jpn"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"0285-9270"},{"subitem_1522646500366":"NCID","subitem_1522646572813":"AN00250763"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"ja","subitem_1522650091861":"琉球大学保健学医学雑誌＝Ryukyu University Journal of Health Sciences and Medicine"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueNumber":"4","bibliographicPageEnd":"300","bibliographicPageStart":"296","bibliographicVolumeNumber":"1"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"v1p296.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2016345/files/v1p296.pdf"},"version_id":"deeb4c9d-f2ba-45d6-95b3-28f1dd5ac9b4"}]},"item_title":"[原著]Deoxyribonuclease Iによる核酸初期分解生成物とb-form核酸との比較","item_type_id":"15","owner":"1","path":["1642838245041","1642838412624"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2014-07-18"},"publish_date":"2014-07-18","publish_status":"0","recid":"2016345","relation_version_is_last":true,"title":["[原著]Deoxyribonuclease Iによる核酸初期分解生成物とb-form核酸との比較"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-02-22T04:55:24.429825+00:00"}