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  1. 学術雑誌論文
  2. 琉球医学会
  3. 琉球大学保健学医学雑誌
  4. 4巻3号
  1. 部局別インデックス
  2. その他

[原著]フィラリア仔虫定期出現性の機序に関する研究(FN-4) : 仔虫自家蛍光顆粒の本態に関する研究(B)

http://hdl.handle.net/20.500.12000/0002016369
http://hdl.handle.net/20.500.12000/0002016369
1c5a96f8-af18-4220-b38f-4dd0212d1e05
名前 / ファイル ライセンス アクション
v4p219.pdf v4p219.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2014-07-18
タイトル
タイトル [原著]フィラリア仔虫定期出現性の機序に関する研究(FN-4) : 仔虫自家蛍光顆粒の本態に関する研究(B)
言語 ja
タイトル
タイトル Studies on The Mechanism of The Microfilarial Periodicity(FN-4) : Studies on the essential feature of the autofluorescent substance(s) in the nocturnalmicronlariae (B)
言語 en
作成者 真喜志, 金造

× 真喜志, 金造

ja 真喜志, 金造

Search repository
Makaishi, Kinzo

× Makaishi, Kinzo

en Makaishi, Kinzo

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
言語 ja
権利情報 琉球医学会
内容記述
内容記述タイプ Other
内容記述 1. The night specimens of Mf. immitis emitted significantly more photons in 5seconds,excited by near 400nm, than those emitted from the daytime specimens. 2. Laser-Raman analysis detected in Mf. immitis the presence of Vitamin A, showing prominent Raman line near 1600cm-1, just samely with that of authentic V. A palmitate. 3. The similarity to rhodopsin and its intermediates was pursued. Microscopic fluorescence spectra, taken using UMSP-1 Zeiss, equipped with a Farrand-fluorescenee recorder, (exited by 365nm and using barrier filter Y42) were very similar among those granules in Mf. bancrofti (SriLanka), earthworm epidermis (head segment) and in the outer rod segments of mouse retma. Masuya (1976), one of the coworkers, has obtained the same microscopic fluorescence spectra, using Nikon SUR-F type, between the earthworm epidermis and in the outer rod segments of Asian toad retina, F max at 510nm and minor F max at 550nm. According to Guzzo & Pool (1969), cattle metarhodopsin II showed F max at 535 (ROS) and 510-515nm (sol), excited by 400nm, and N-retinyline opsin at 506-510nm (ROS). excited by 360nm. Absorption max of metarhodopsin 2 is known to be 380nm and that of RNO to be 44Onm in acid and 365nm in alkali. Absorption spectra of those granules in Mf. bancrofti (UMSP- 1 Zeiss) showed $ \lambda $max at 406nm, apart from that of Hb. Under the same conditions, those granules both in the earthworm epidermis and in the outer rod segments of mouse retina showed $ \lambda $max near 414nm and shoulder near 540nm The granules both in Mf. bancrofti and in the earthworm epidermis showed $ \lambda $max near 413nm at pH 5.7and near 406nm at 8.6, thus showing a little shift toward shorter wave length. However, the granules in the outer rod segments of mouse retina gave the same $ \lambda $max at pH 5.7,7.7 and 8.6 (411.6nm) ; although those in native retina at 412.4nm, in the same specimens, on the sameday, thus showing no shift of $ \lambda $max,by pH. Those findings were compared with the known characteristics of rhodopsin and its intermediates ($ \lambda $max, excitation wave length and F max.) So far as concerned with findings, obtained, of microscopic fluorescence spectra and absorption spectra, after light exposure and at room temperature, the auto fluorescent granules in the nocturnal microfilariae and in the earthworm epidermis, both negative phototactic, were considered to be similar to metarhodopsin II or N-retinylidene opsin, among the bleached intermediates of the visual pigments. Further, it must be taken into consideration that $ \lambda $max of the visual pigments distribute from 520nm to 437nm in the insects and arachnid, examined, shown in the literature. It was discussed on the possible harmful effects of rhodopsin, its photodynamic activity at least as photosensitizer, in the presence of light and oxygen, recalling retrolental fibroplasia in the prematurity and ecliptic fetinitis.
内容記述
内容記述タイプ Other
内容記述 論文
出版者
言語 ja
出版者 琉球大学医学部
出版者
言語 en
出版者 Faculty of Medicine, University of the Ryukyus
言語
言語 jpn
資源タイプ
資源タイプ journal article
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0285-9270
収録物識別子
収録物識別子タイプ NCID
収録物識別子 AN00250763
収録物名
言語 ja
収録物名 琉球大学保健学医学雑誌=Ryukyu University Journal of Health Sciences and Medicine
書誌情報
巻 4, 号 3, p. 219-237
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