@article{oai:u-ryukyu.repo.nii.ac.jp:02016369,
 author = {真喜志, 金造 and Makaishi, Kinzo},
 issue = {3},
 journal = {琉球大学保健学医学雑誌＝Ryukyu University Journal of Health Sciences and Medicine},
 note = {1. The night specimens of Mf. immitis emitted significantly more photons in 5seconds,excited by near 400nm, than those emitted from the daytime specimens. 2. Laser-Raman analysis detected in Mf. immitis the presence of Vitamin A, showing prominent Raman line near 1600cm-1, just samely with that of authentic V. A palmitate. 3. The similarity to rhodopsin and its intermediates was pursued. Microscopic fluorescence spectra, taken using UMSP-1 Zeiss, equipped with a Farrand-fluorescenee recorder, (exited by 365nm and using barrier filter Y42) were very similar among those granules in Mf. bancrofti (SriLanka), earthworm epidermis (head segment) and in the outer rod segments of mouse retma. Masuya (1976), one of the coworkers, has obtained the same microscopic fluorescence spectra, using Nikon SUR-F type, between the earthworm epidermis and in the outer rod segments of Asian toad retina, F max at 510nm and minor F max at 550nm. According to Guzzo & Pool (1969), cattle metarhodopsin II showed F max at 535 (ROS) and 510-515nm (sol), excited by 400nm, and N-retinyline opsin at 506-510nm (ROS). excited by 360nm. Absorption max of metarhodopsin 2 is known to be 380nm and that of RNO to be 44Onm in acid and 365nm in alkali. Absorption spectra of those granules in Mf. bancrofti (UMSP- 1 Zeiss) showed $ \lambda $max at 406nm, apart from that of Hb. Under the same conditions, those granules both in the earthworm epidermis and in the outer rod segments of mouse retina showed $ \lambda $max near 414nm and shoulder near 540nm The granules both in Mf. bancrofti and in the earthworm epidermis showed $ \lambda $max near 413nm at pH 5.7and near 406nm at 8.6, thus showing a little shift toward shorter wave length. However, the granules in the outer rod segments of mouse retina gave the same $ \lambda $max at pH 5.7,7.7 and 8.6 (411.6nm) ; although those in native retina at 412.4nm, in the same specimens, on the sameday, thus showing no shift of $ \lambda $max,by pH. Those findings were compared with the known characteristics of rhodopsin and its intermediates ($ \lambda $max, excitation wave length and F max.) So far as concerned with findings, obtained, of microscopic fluorescence spectra and absorption spectra, after light exposure and at room temperature, the auto fluorescent granules in the nocturnal microfilariae and in the earthworm epidermis, both negative phototactic, were considered to be similar to metarhodopsin II or N-retinylidene opsin, among the bleached intermediates of the visual pigments. Further, it must be taken into consideration that $ \lambda $max of the visual pigments distribute from 520nm to 437nm in the insects and arachnid, examined, shown in the literature. It was discussed on the possible harmful effects of rhodopsin, its photodynamic activity at least as photosensitizer, in the presence of light and oxygen, recalling retrolental fibroplasia in the prematurity and ecliptic fetinitis., 論文},
 pages = {219--237},
 title = {[原著]フィラリア仔虫定期出現性の機序に関する研究(FN-4) : 仔虫自家蛍光顆粒の本態に関する研究(B)},
 volume = {4}
}