@article{oai:u-ryukyu.repo.nii.ac.jp:02016375,
 author = {与都嶺, 毅 and Yonamine, Tsuyoshi},
 issue = {4},
 journal = {琉球大学保健学医学雑誌＝Ryukyu University Journal of Health Sciences and Medicine},
 note = {Scanning electronmicroscopic studies were done using frozen fracture method on Mf immitis in the peripheral blood, intrauterine embryos and Mf. in the dogs, infected with Dipetalonema reconditum and with Brugia pahangi. Mf immitis in the peripheral blood and some larvae of Dipetalonewa reconditum showed several globules of variable sizes (0.1 to 0.35 $ \mu $ in each fractured surface. Those larvae had shown autofluorescent granules under fluorescence microscope. The intrauterine Mf immitis and the other Mf. reconditum, those had not shown any fluorescent granule, showed no such globule in any fractured surface of any larva. Some larvae of Brugia pahangi showed no such globule, and on the background near the other mf. were found minute globules. It is very likely that those globules correspond to the autofluorescent granules. Further analysis could not be done, because of the difficulty to use extremely expensive apparatus. The negative phototaxis of Mf. immitis was demonstrated in vitro. Heparinized and hemolyzed canine blood was washed twice or thrice with saline and the sediment was suspended in the host's serum. Such suspension was put in Petri dish, half of which, covered with plastic lid, which again covered with aluminum foil. UV-illumination was done from 10 cm over the lid. Pipeting the sample, 0.02 ml, was done every10-15minutes, from both the bright and dark parts, for mf-counting. After 15 minutes of illumination, two times more mf. and after 120 minutes, ten times more mf. were detected in the dark parts than in the bright parts. In another series of observation, suspension was put between two pieces of thin slide glass, half of the length of each piece, painted with black or colored ink or covered with colored cellophane. Strong illumination was done from the bottom. At the beginning of, observation, mf. were distributed evenly in both the bright and dark parts, while more mf. were seen in the dark parts after 15-30 minutes (in some cases within 10 seconds) of illumination. Such evasion of mf. toward the dark parts could be demonstrated by means of photomicrography and cinemicrography. Such evasion of mf. from the bright parts was seen toward black, red, orange and yellow parts, while no evasion was seen toward green and blue parts., 論文},
 pages = {335--355},
 title = {[原著]フィラリア仔虫定期出現性の機序に関する研究(FN-2)},
 volume = {2}
}