{"created":"2022-02-22T04:57:48.459657+00:00","id":2016428,"links":{},"metadata":{"_buckets":{"deposit":"d2f99376-f4ab-4440-a142-40c0baaf6062"},"_deposit":{"id":"2016428","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2016428"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02016428","sets":["1642838163960:1642838198944:1642838242961:1642838244566","1642838403551:1642838412624"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"[原著]ウェルシュ菌 K29 株の産生するバクテリオシンおよび溶菌物質の精製とその性状","subitem_1551255648112":"ja"},{"subitem_1551255647225":"Partial purification and some properties of the bacteriocin and the bacteriolytic substance produced by Clostridium perfringens strain K29","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"吉元, 房枝","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"今村, 禎祐","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Yoshimoto, Fusae","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Imamura, Teisuke","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_1522650717957":"ja","subitem_1522651041219":"琉球医学会"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Substances inhibiting the growth of sensitive cells and lysing them, produced by Clostridium perfringens strain K29 were separated in high purity by 50% ammonium sulfate precipitation, gel filtration on Sephadex G-100 and ion exchange chromatography on DEAE Sephadex A-50. The two substances obtained were compared for physicochemical properties and the effect on sensitive cells. At the range from pH 7 to 8, the inhibitory substance was stable, whereas the bacteriolytic substance was stable at the range from pH 5 to 8. Both substances were inactivated by proteinase and by heat treatment for 5 minutes at 50℃. The effect of the inhibitory substance appeared to be bactericidal but not bacteriolytic, whereas the bacteriolytic substance lysed sensitive cells rapidly. According to this investigation, it is satisfactory to consider the former as a bacteriocin and the latter as a lytic enzyme.","subitem_description_type":"Other"},{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"琉球大学保健学部"},{"subitem_1522300295150":"en","subitem_1522300316516":"College of Health Sciences, University of the Ryukyus"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"jpn"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"0285-9270"},{"subitem_1522646500366":"NCID","subitem_1522646572813":"AN00250763"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"ja","subitem_1522650091861":"琉球大学保健学医学雑誌＝Ryukyu University Journal of Health Sciences and Medicine"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueNumber":"3","bibliographicPageEnd":"197","bibliographicPageStart":"185","bibliographicVolumeNumber":"1"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"v1p185.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2016428/files/v1p185.pdf"},"version_id":"1cd5c4c0-d998-4d40-bba3-01501d5b5179"}]},"item_title":"[原著]ウェルシュ菌 K29 株の産生するバクテリオシンおよび溶菌物質の精製とその性状","item_type_id":"15","owner":"1","path":["1642838244566","1642838412624"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2014-07-18"},"publish_date":"2014-07-18","publish_status":"0","recid":"2016428","relation_version_is_last":true,"title":["[原著]ウェルシュ菌 K29 株の産生するバクテリオシンおよび溶菌物質の精製とその性状"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-02-22T04:57:51.878754+00:00"}