@article{oai:u-ryukyu.repo.nii.ac.jp:02016471,
 author = {Matsui, Katsuaki and Yumoto, Tokichi and Nashiro, Junko and Hashimoto, Kimio and Furuse, Kiyoo and Morimoto, Kanehito and Akeho, Masahiro and Maeyama, Iwao},
 issue = {3},
 journal = {琉球大学保健学医学雑誌＝Ryukyu University Journal of Health Sciences and Medicine},
 note = {Establishment of the cell line ( HOS-O ) of osteosarcoma was successfully accomplished. The materials for this study were obtained from surgically removed tumor mass metastasized to the lung The patient was a 10 year-old girl, suffering from an original tumor in the right distal femur. The tissue culture has been started in September 1977, and the cell line has been maintained in a good condition for about five years. The shape of cultured cells is elongated-spindle and its chromosome number is 46. By histochemical examination, the cells revealed weakly positive at alkaline phosphatase, and moderately positive at acid phosphatase, and highly positive at nonspecific esterase and 5'-nucleotidase. Each of twice transplantations of those cells, to the back of nude mice, was successful. Histological features of the trasplanted tumors were resembled to that of the original sarcoma, showing a remarkable formation of osteoid tissue., 論文},
 pages = {187--195},
 title = {[原著］Establishment of a Cell Line Derived from Human Osteosarcoma},
 volume = {5}
}