{"created":"2022-02-22T06:17:04.138856+00:00","id":2016560,"links":{},"metadata":{"_buckets":{"deposit":"528d16ab-9ea0-4a3a-8f5a-7a239e032bdb"},"_deposit":{"id":"2016560","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2016560"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02016560","sets":["1642838163960:1642838253200:1642838253649:1642838259014","1642838403551:1642838412624"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"[報文]カラシナのカルス由来プロトプラストからの不定芽再分化","subitem_1551255648112":"ja"},{"subitem_1551255647225":"[ORIGINALS]Shoot Regeneration from Protoplasts Derived from Callus of Brassica Juncea","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"徳元, 正和","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"TOKUMOTO, Masakazu","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_1522650717957":"ja","subitem_1522651041219":"南方資源利用技術研究会"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"力ラシナカルスから単離したプロトプラストを用いて,初期分裂に及ぼす基本培地濃度,窒素塩濃度の影響,ホルモン濃度の影響,さらに不定芽再分化に対する各種添加物の効果について検討した.カラシナプロトプラストは浸透圧調節剤のマンニトール0.475～0.5Mの範囲で安定であった｡プロトプラストの初期分裂に対して,BA1mｇ/ lとNAAO.5mg/lのホルモン組合せで効果が高く,分裂率は29.4%であった. また,修正MS培地(NH_4NO_3を(NH_4)_2S0_4に置換)の窒素源として(NH_4)_2SO_4は200mg/l, KN0_3は475mg/lの濃度でもっとも高い分裂率が得られ,それぞれ34.7%,36.2%であった.修正MS培地中の無機塩濃度(窒素源は除く)は1/2濃度が効果的であった.得られた最適条件の培地に,活性炭(0.035%),カザミノ酸(0.025%),コンデショニング培地(50%)を添加して,プロトプラスト培養をすると,活性炭以外はコロニーを経てカルスが形成された.特にコンデショニング培地ではコロニーおよびカルス形成効果が高く,得られたカルスを再分化培地に移植すると,コンデショニング培地で不定芽形成(形成率12%)が見られた.しかし,他の培地では不定芽は形成されず不定根のみ形成された.","subitem_description_type":"Other"},{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"南方資源利用技術研究会"},{"subitem_1522300295150":"en","subitem_1522300316516":"The Society of Tropical Resources Technologists"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"jpn"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"0912-9588"},{"subitem_1522646500366":"NCID","subitem_1522646572813":"AN10236281"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"ja","subitem_1522650091861":"南方資源利用技術研究会誌 = Journal of the society tropical resources technologists"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueNumber":"1","bibliographicPageEnd":"6","bibliographicPageStart":"1","bibliographicVolumeNumber":"10"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"Vol10No1p1.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2016560/files/Vol10No1p1.pdf"},"version_id":"78593f00-3fb4-4e81-9fe7-3542ce190ad9"}]},"item_title":"[報文]カラシナのカルス由来プロトプラストからの不定芽再分化","item_type_id":"15","owner":"1","path":["1642838259014","1642838412624"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2014-10-26"},"publish_date":"2014-10-26","publish_status":"0","recid":"2016560","relation_version_is_last":true,"title":["[報文]カラシナのカルス由来プロトプラストからの不定芽再分化"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-02-22T06:17:07.299146+00:00"}