{"created":"2022-02-24T07:48:02.679200+00:00","id":2017765,"links":{},"metadata":{"_buckets":{"deposit":"bf0ad481-e52d-415f-a736-21e35aa8799b"},"_deposit":{"id":"2017765","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2017765"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02017765","sets":["1642838163960:1642838198944:1642838199408:1642838229091","1642838403551:1642838412624"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"[原著]Type II interleukin-4 receptor-mediated anti-inflammatory response in Mm1 and J774.1 macrophages","subitem_1551255648112":"ja"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Lin, Yanhui","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kato, Seiya","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_1522650717957":"ja","subitem_1522651041219":"琉球医学会"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"IL-4"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"IL-13"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"receptor"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"macrophage"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"inflammation"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Introduction: Macrophages are involved in various inflammatory processes. Although the therapeutic potential of interleukin-4 (IL-4)-mediated immunomodulation has been proposed, it has not yet been established. The type I IL-4 receptor (IL-4RI) consists of IL-4R α and a common γ chain, whereas the type II IL-4R (IL-4RII) is a heterodimer of IL-4R a and IL-13R α 1. In the present study, we assessed the selective activation of IL-4R isoforms in the mouse macrophage cell lines, Mm1 and J774.1, to investigate the role of receptor-specific signaling in inflammatory activation. Materials and Methods: The plasmid expressing murine IL-4/ Q116E, which is analogous to the human IL-4RI-specific agonist IL-4/ R121E, was transfected to assess IL-4RI activation. IL-4RII activation was induced by IL-13. Activation of IL-4R isoforms was confirmed by the activation of signal transducer and activator of transcription 6 (STAT6). Lipopolysaccharide (LPS)-induced tumor necrosis factor-α (TNF-α) induction was examined using real-time polymerase chain reaction. LPS plus interferon- γ (INF- γ )-stimulated inducible nitric oxide synthase induction was tested using western blotting. Results: IL-4/ Q116E induced STAT6 activation in Mm1 cells but not in J774.1 cells. In Mm1 cells, IL-4/ Q116E-induced STAT6 activation was inhibited by kaempferol, a specific inhibitor for Janus kinase 3 (JAK3). IL-4/Q116E did not exhibit anti-inflammatory activity in either macrophage cell line. However, IL-13 inhibited inflammatory activation of these cells. Conclusions: Our data suggest that Mm1 cells expressed both IL-4RI and IL-4RII, whereas J774.1 cells expressed only IL-4RII. Although IL-4R isoform expression pattern was diverse in these two macrophages, the IL-4-mediated antiinflammatory effect was thought to be largely dependent on IL-4RII-mediated signaling. Thus, we could further conclude that IL-4 signaling plays a pivotal role in the regulation of macrophage inflammatory proprieties. Understanding IL-4-mediated anti-inflammatory signaling in macrophages may be beneficial for the development of therapeutics.","subitem_description_type":"Other"},{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"琉球医学会"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"1346-888X"},{"subitem_1522646500366":"NCID","subitem_1522646572813":"AN10369445"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"ja","subitem_1522650091861":"琉球医学会誌 = Ryukyu Medical Journal"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueNumber":"3･4","bibliographicPageEnd":"78","bibliographicPageStart":"69","bibliographicVolumeNumber":"32"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"v3234p69.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2017765/files/v3234p69.pdf"},"version_id":"c32d301b-15e8-4e45-b22b-04d2f49ad0e7"}]},"item_title":"[原著]Type II interleukin-4 receptor-mediated anti-inflammatory response in Mm1 and J774.1 macrophages","item_type_id":"15","owner":"1","path":["1642838229091","1642838412624"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-04-24"},"publish_date":"2015-04-24","publish_status":"0","recid":"2017765","relation_version_is_last":true,"title":["[原著]Type II interleukin-4 receptor-mediated anti-inflammatory response in Mm1 and J774.1 macrophages"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-02-24T07:48:08.169446+00:00"}