{"created":"2022-02-24T07:48:08.359518+00:00","id":2017767,"links":{},"metadata":{"_buckets":{"deposit":"3b20a6f6-0e84-4b38-8ef1-e929e84fbbc9"},"_deposit":{"id":"2017767","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2017767"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02017767","sets":["1642838163960:1642838198944:1642838199408:1642838229091","1642838403551:1642838412624"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"[原著]Phenotypic changes in a squamous cell carcinoma cell line induced by an effector kinase of a small G protein Rap2","subitem_1551255648112":"ja"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ulziikhishig, Enkhbaatar","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Bayarjargal, Maitsetseg","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nonaka, Kimiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Oshiro, Minoru","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kariya, Ken-ichi","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_1522650717957":"ja","subitem_1522651041219":"琉球医学会"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"cancer cell phenotype"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Rap2"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"MAP4K"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"skin cancer"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Purpose: Traf2- and Nck-interacting kinase (TNIK) is one of the effector kinases of the Ras-like GTPase Rap2, and belongs to the STE20 group of mitogen-activated protein kinase kinase kinase kinases (MAP4Ks). The purpose of this study was to explore the roles of TNIK in the phenotype of cancer cells. Results: Derivatives of a cutaneous squamous cell carcinoma (SCC) cell line, Pam212, in which expression of hemagglutinin-tagged TNIK (HA-TNIK) can be induced by modulating the concentration of a tetracycline derivative in culture medium were established by using a retrovirus-mediated gene transduction system. Morphological analyses revealed that HA-TNIK regulated intercellular adhesion and epithelial cell shape. A strong induction of HA-TNIK disrupted intercellular adhesion and abolished the epithelial cell shape. Moderately induced HA-TNIK co-localized with E-cadherin, a principal component of intercellular adherens junctions (AJs), and facilitated cellular detachment during hepatocyte growth factor-induced cell scattering and wound-healing cell migration assays. Moderately induced HA-TNIK also retarded Ca^<2_+>-dependent re-assembly of AJs that had been pre-disassembled by transient depletion of Ca^<2_+> in culture medium. Conclusion: These results complement our preceding work using a proteomic approach (Kuninaka et al. \"Proteomic changes in a squamous cell carcinoma cell line induced by an effector kinase of a small G protein Rap2\" Ryukyu Med. J. 26: 135-145, 2007) on roles of TNIK in SCC cells.","subitem_description_type":"Other"},{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"琉球医学会"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"1346-888X"},{"subitem_1522646500366":"NCID","subitem_1522646572813":"AN10369445"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"ja","subitem_1522650091861":"琉球医学会誌 = Ryukyu Medical Journal"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueNumber":"3･4","bibliographicPageEnd":"94","bibliographicPageStart":"89","bibliographicVolumeNumber":"32"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"v3234p89.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2017767/files/v3234p89.pdf"},"version_id":"fe0668c2-68bb-4d90-a4d6-5cc8c575aa90"}]},"item_title":"[原著]Phenotypic changes in a squamous cell carcinoma cell line induced by an effector kinase of a small G protein Rap2","item_type_id":"15","owner":"1","path":["1642838229091","1642838412624"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-05-08"},"publish_date":"2015-05-08","publish_status":"0","recid":"2017767","relation_version_is_last":true,"title":["[原著]Phenotypic changes in a squamous cell carcinoma cell line induced by an effector kinase of a small G protein Rap2"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-02-24T07:48:13.178843+00:00"}