{"created":"2022-06-10T04:10:44.061335+00:00","id":2018035,"links":{},"metadata":{"_buckets":{"deposit":"00e8a295-cdb4-4a59-86d2-76135ec62c02"},"_deposit":{"created_by":8,"id":"2018035","owner":"8","owners":[8],"owners_ext":{"displayname":"ryukyu_lib","username":null},"pid":{"revision_id":0,"type":"depid","value":"2018035"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02018035","sets":["1642838158423:1642838158860:1642838159351","1642838403551:1642838409905"]},"author_link":[],"control_number":"2018035","item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"GRIM-19 is a target of mycobacterial Zn^<2+> metalloprotease 1 and indispensable for NLRP3 inflammasome activation","subitem_1551255648112":"en"},{"subitem_1551255647225":"GRIM-19は結核菌のZn^<2+>メタロプロテアーゼの標的であり、NLRP3インフラマソームの活性化に必須である","subitem_1551255648112":"ja"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"藏根, 友美","creatorNameLang":"ja"},{"creatorName":"Kurane, Tomomi","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_1522650717957":"en","subitem_1522651041219":"© 2021 Federation of American Societies for Experimental Biology"},{"subitem_1522650717957":"en","subitem_1522650727486":"https://creativecommons.org/licenses/by-nc/4.0/","subitem_1522651041219":"Creative Commons Attribution-NonCommercial 4.0 International"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"inflammasome"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"interleukin-1β"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"macrophages"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"mitochondria"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Mycobacterium tuberculosis"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Tuberculosis is a communicable disease caused by Mycobacterium tuberculosis which primarily infects macrophages and establishes intracellular parasitism. A mycobacterial virulence factor Zn^<2+> metalloprotease 1 (Zmp1) is known to suppress interleukin (IL)-1β production by inhibiting caspase-1 resulting in phagosome maturation arrest. However, the molecular mechanism of caspase-1 inhibition by Zmp1 is still elusive. Here, we identified GRIM-19 (also known as NDUFA13), an essential subunit of mitochondrial respiratory chain complex I, as a novel Zmp1-binding protein. Using the CRISPR/Cas9 system, we generated GRIM-19 knockout murine macrophage cell line J774.1 and found that GRIM-19 is essential for IL-1β production during mycobacterial infection as well as in response to NLRP3 inflammasome-activating stimuli such as extracellular ATP or nigericin. We also found that GRIM-19 is required for the generation of mitochondrial reactive oxygen species and NLRP3-dependent activation of caspase-1. Loss of GRIM-19 or forced expression of Zmp1 resulted in a decrease in mitochondrial membrane potential. Our study revealed a previously unrecognized role of GRIM-19 as an essential regulator of NLRP3 inflammasome and a molecular mechanism underlying Zmp1-mediated suppression of IL-1β production during mycobacterial infection.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"琉球大学"},{"subitem_1522300295150":"en","subitem_1522300316516":"University of the Ryukyus"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"PISSN","subitem_1522646572813":"0892-6638"},{"subitem_1522646500366":"EISSN","subitem_1522646572813":"1530-6860"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"en","subitem_1522650091861":"FASEB Journal"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueNumber":"1","bibliographicVolumeNumber":"36"}]},"item_1617187087799":{"attribute_name":"Dissertation Number","attribute_value_mlt":[{"subitem_1551256171004":"甲第541号"}]},"item_1617187112279":{"attribute_name":"Degree Name","attribute_value_mlt":[{"subitem_1551256126428":"博士(医学)","subitem_1551256129013":"ja"}]},"item_1617187136212":{"attribute_name":"Date Granted","attribute_value_mlt":[{"subitem_1551256096004":"2022-03-18"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_1522306287251":{"subitem_1522306382014":"DOI","subitem_1522306436033":"https://doi.org/10.1096/fj.202101074RR"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2022-06-10"}],"filename":"iken541abstract.pdf","filesize":[{"value":"787 KB"}],"mimetype":"application/pdf","url":{"objectType":"abstract","url":"https://u-ryukyu.repo.nii.ac.jp/record/2018035/files/iken541abstract.pdf"},"version_id":"6e7baf63-6d96-4a74-b3e1-0e7fc0f89a76"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2022-06-10"}],"filename":"iken541review.pdf","filesize":[{"value":"1023 KB"}],"mimetype":"application/pdf","url":{"objectType":"other","url":"https://u-ryukyu.repo.nii.ac.jp/record/2018035/files/iken541review.pdf"},"version_id":"8f0cfe3b-9961-44b9-8a3d-c236523dbc55"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2022-06-10"}],"filename":"iken541text.pdf","filesize":[{"value":"10.6 MB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2018035/files/iken541text.pdf"},"version_id":"ad88a33a-0737-431b-854f-aa9f76f5d58f"}]},"item_1617944105607":{"attribute_name":"Degree Grantor","attribute_value_mlt":[{"subitem_1551256015892":[{"subitem_1551256027296":"18001","subitem_1551256029891":"kakenhi"}],"subitem_1551256037922":[{"subitem_1551256042287":"琉球大学","subitem_1551256047619":"ja"}]}]},"item_title":"GRIM-19 is a target of mycobacterial Zn^<2+> metalloprotease 1 and indispensable for NLRP3 inflammasome activation","item_type_id":"59","owner":"8","path":["1642838159351","1642838409905"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-06-10"},"publish_date":"2022-06-10","publish_status":"0","recid":"2018035","relation_version_is_last":true,"title":["GRIM-19 is a target of mycobacterial Zn^<2+> metalloprotease 1 and indispensable for NLRP3 inflammasome activation"],"weko_creator_id":"8","weko_shared_id":-1},"updated":"2022-06-10T05:06:31.979296+00:00"}