{"created":"2022-06-15T06:29:08.056898+00:00","id":2018043,"links":{},"metadata":{"_buckets":{"deposit":"38f23e4b-2a0e-4c07-bde8-3f42a07e62e3"},"_deposit":{"created_by":8,"id":"2018043","owner":"8","owners":[8],"pid":{"revision_id":0,"type":"depid","value":"2018043"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02018043","sets":["1642838158423:1642838158860:1642838159351","1642838403551:1642838409905"]},"author_link":[],"control_number":"2018043","item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"A new diagnostic algorithm using biopsy specimens in adult T-cell leukemia/lymphoma: combination of RNA in situ hybridization and quantitative PCR for HTLV-1","subitem_1551255648112":"en"},{"subitem_1551255647225":"成人 T 細胞白血病・リンパ腫における生検組織を用いた新規診断アルゴリズム ： HTLV-1 に対する RNA in situ hybridization と定量 PCR の組み合わせ","subitem_1551255648112":"ja"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"髙鳥, 光徳","creatorNameLang":"ja"},{"creatorName":"Takatori, Mitsuyoshi","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_1522650717957":"en","subitem_1522651041219":"© The Author(s) 2020."},{"subitem_1522650717957":"en","subitem_1522650727486":"http://creativecommons.org/licenses/by/4.0/","subitem_1522651041219":"Creative Commons  Attribution 4.0 International"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Histopathological distinction between adult T-cell leuketnia/lymphoma (ATLL) and other T-cell neoplasms is often challenging. The current gold standard for the accurate diagnosis of ATLL is the Southern blot hybridization (SBH) assay, which detects clonal integration of human T-cell leuketnia virus type I (HTLV-1) provirus. However, SBH cannot be petformed with small biopsy or formalin-fixed paraffin-embedded (FFPE) tissue samples because this assay requires a large amount of DNA without degradation. Here we developed a new diagnostic algorithm for the accurate diagnosis of ATLL using FFPE samples. This method combines two HTL V-1 detection assays, namely, ultrasensitive RNA in situ hybridization using RNAscope for HTLV-1 bZIP factor (HBZ-RNAscope), and quantitative PCR targeting the tax gene (tax-qPCR). We analyzed 119 FFPE tissue specimens (62 ATLL, and 57 non-ATLL, including 41 HTLV-1 carriers) and compared them with the SBH results using the corresponding fresh-frozen samples. As a result, tax-qPCR had a higher ATLL identification rate  than HBZ-RNAscope (88% [52/59], and 63% [39/62], respectively). However, HBZ-RNAscope clearly visualized the localization of HTLV-1-infected tumor cells and its identification rate increased to 94% (17/18) when the analysis was limited to samples up to 2 years old, indicating its usefulness in the daily diagnosis. The diagnostic algorithm combining these two assays successfully evaluated 94% (112/119) of samples and distinguished ATLL from non-ATLL cases including HTLV-1 carriers with 100% sensitivity and specificity. This method is expected to replace SBH and increase the accuracy of the diagnosis of A TLL.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"琉球大学"},{"subitem_1522300295150":"en","subitem_1522300316516":"University of the Ryukyus"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"PISSN","subitem_1522646572813":"0893-3952"},{"subitem_1522646500366":"EISSN","subitem_1522646572813":"1530-0285"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"en","subitem_1522650091861":"Modern Pathology"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicPageEnd":"58","bibliographicPageStart":"51","bibliographicVolumeNumber":"34"}]},"item_1617187087799":{"attribute_name":"Dissertation Number","attribute_value_mlt":[{"subitem_1551256171004":"甲第547号"}]},"item_1617187112279":{"attribute_name":"Degree Name","attribute_value_mlt":[{"subitem_1551256126428":"博士(医学)","subitem_1551256129013":"ja"}]},"item_1617187136212":{"attribute_name":"Date Granted","attribute_value_mlt":[{"subitem_1551256096004":"2022-03-18"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_1522306287251":{"subitem_1522306382014":"DOI","subitem_1522306436033":"https://doi.org/10.1038/s41379-020-0635-8"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2022-06-15"}],"filename":"iken547abstract.pdf","filesize":[{"value":"875 KB"}],"mimetype":"application/pdf","url":{"objectType":"abstract","url":"https://u-ryukyu.repo.nii.ac.jp/record/2018043/files/iken547abstract.pdf"},"version_id":"d139be97-d085-4c97-8d6a-ed7cc2c169bf"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2022-06-15"}],"filename":"iken547review.pdf","filesize":[{"value":"803 KB"}],"mimetype":"application/pdf","url":{"objectType":"other","url":"https://u-ryukyu.repo.nii.ac.jp/record/2018043/files/iken547review.pdf"},"version_id":"983d48cb-da1e-4637-a54d-9b14cafebe21"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2022-06-15"}],"filename":"iken547text.pdf","filesize":[{"value":"3.1 MB"}],"mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2018043/files/iken547text.pdf"},"version_id":"65bcac1d-8d1d-48db-887e-66d58ba8bc98"}]},"item_1617944105607":{"attribute_name":"Degree Grantor","attribute_value_mlt":[{"subitem_1551256015892":[{"subitem_1551256027296":"18001","subitem_1551256029891":"kakenhi"}],"subitem_1551256037922":[{"subitem_1551256042287":"琉球大学","subitem_1551256047619":"ja"}]}]},"item_title":"A new diagnostic algorithm using biopsy specimens in adult T-cell leukemia/lymphoma: combination of RNA in situ hybridization and quantitative PCR for HTLV-1","item_type_id":"59","owner":"8","path":["1642838159351","1642838409905"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-06-16"},"publish_date":"2022-06-16","publish_status":"0","recid":"2018043","relation_version_is_last":true,"title":["A new diagnostic algorithm using biopsy specimens in adult T-cell leukemia/lymphoma: combination of RNA in situ hybridization and quantitative PCR for HTLV-1"],"weko_creator_id":"8","weko_shared_id":-1},"updated":"2022-07-04T02:33:13.916603+00:00"}