{"created":"2022-10-06T06:11:36.757384+00:00","id":2019518,"links":{},"metadata":{"_buckets":{"deposit":"dd9c56a8-4961-40b2-80b2-f0467a2843ec"},"_deposit":{"created_by":14,"id":"2019518","owner":"14","owners":[14],"owners_ext":{"displayname":"kugai","username":null},"pid":{"revision_id":0,"type":"depid","value":"2019518"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02019518","sets":["1642838158423:1642838158860:1642838159351","1642838403551:1642838409905"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"Slow progression of sciatic nerve degeneration and regeneration after loose ligation through microglial activation and decreased KCC2 levels in the mouse spinal cord ventral horn","subitem_1551255648112":"en"},{"subitem_1551255647225":"マウス脊髄前角におけるミクログリアの活性化とKCC2 の発現低下を介した緩い坐 骨神経結紮後の神経変性及び再生の緩徐な進行","subitem_1551255648112":"ja"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"屋富祖, 司","creatorNameLang":"ja"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Peripheral nerve injury affects motor functions. To reveal the mechanisms underlying motor dysfunction and recoveryafter nerve compression, which have not been precisely examined, we investigated the temporal relationshipamong changes in motor function, nerve histopathology, and marker molecule expression in the spinal cordafter loose ligation of the mouse sciatic nerve. After ligation, sciatic motor function suddenly declined, and axonsgradually degenerated. During degeneration, galanin was localized in motor neuron cell bodies. Then, in the ventralhorn, microglia were activated, and expression of choline acetyltransferase (ChAT), a synthetic enzyme ofacetylcholine, and potassium chloride co-transporter 2 (KCC2), which shifts the action of γ-amino butyric acid(GABA) and glycine to inhibitory, decreased. Motor function recovery was insufficient although axonal regenerationwas complete. ChAT levels gradually recovered during axonal regeneration. When regeneration was nearlycomplete, microglial activation declined, and KCC2 expression started to increase. The KCC2 level sufficiently recoveredwhen axonal regeneration was complete, suggesting that the excitatory action of GABA/glycine mayparticipate in axonal regeneration. Furthermore, these changes proceeded slower than those after severance,suggesting that loose ligation, compression, may mediate slower progression of degeneration and regenerationthan severance, and these changes may cause the motor dysfunction and its recovery.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"琉球大学"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"eng"}]},"item_1617187087799":{"attribute_name":"Dissertation Number","attribute_value_mlt":[{"subitem_1551256171004":"甲第536号"}]},"item_1617187112279":{"attribute_name":"Degree Name","attribute_value_mlt":[{"subitem_1551256126428":"博士(医学)","subitem_1551256129013":"ja"}]},"item_1617187136212":{"attribute_name":"Date Granted","attribute_value_mlt":[{"subitem_1551256096004":"2021-11-04"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2022-10-06"}],"filename":"iken536abstract.pdf","filesize":[{"value":"1.4 MB"}],"url":{"objectType":"abstract","url":"https://u-ryukyu.repo.nii.ac.jp/record/2019518/files/iken536abstract.pdf"},"version_id":"4083e6c0-a603-4cc5-87f0-bea1f8f0834b"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2022-10-06"}],"filename":"iken536review.pdf","filesize":[{"value":"1.1 MB"}],"url":{"objectType":"other","url":"https://u-ryukyu.repo.nii.ac.jp/record/2019518/files/iken536review.pdf"},"version_id":"48ae8f92-0771-4c0d-9d15-083084c61ab2"}]},"item_1617944105607":{"attribute_name":"Degree Grantor","attribute_value_mlt":[{"subitem_1551256015892":[{"subitem_1551256027296":"18001","subitem_1551256029891":"kakenhi"}],"subitem_1551256037922":[{"subitem_1551256042287":"琉球大学","subitem_1551256047619":"ja"}]}]},"item_title":"Slow progression of sciatic nerve degeneration and regeneration after loose ligation through microglial activation and decreased KCC2 levels in the mouse spinal cord ventral horn","item_type_id":"59","owner":"14","path":["1642838159351","1642838409905"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-08-31"},"publish_date":"2022-08-31","publish_status":"0","recid":"2019518","relation_version_is_last":true,"title":["Slow progression of sciatic nerve degeneration and regeneration after loose ligation through microglial activation and decreased KCC2 levels in the mouse spinal cord ventral horn"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2022-10-06T06:17:49.802803+00:00"}