{"created":"2023-03-08T05:48:07.782313+00:00","id":2019637,"links":{},"metadata":{"_buckets":{"deposit":"cae04b6f-ff6c-493e-be8f-2de76cafb5ca"},"_deposit":{"created_by":8,"id":"2019637","owner":"8","owners":[8],"owners_ext":{"displayname":"ryukyu_lib","username":null},"pid":{"revision_id":0,"type":"depid","value":"2019637"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02019637","sets":["1642838163960:1642838338003","1642838403551:1642838407795"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"Comparison of Tissue Loading Before and After the Creation of a Continuous Density Gradient in Porcine Islet Purification","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Miyagi-Shiohira, Chika","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nakashima, Yoshiki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ebi, Nana","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hamada, Eri","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Tamaki, Yoshihito","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kuwae, Kazuho","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kobayashi, Naoya","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Saitoh, Issei","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Watanabe, Masami","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kinjo, Takao","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Noguchi, Hirofumi","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_1522650717957":"en","subitem_1522651041219":"© The Author(s) 2018"},{"subitem_1522650717957":"en","subitem_1522650727486":"http://www.creativecommons.org/licenses/by-nc/4.0/","subitem_1522651041219":"This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage)."}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"bottle purification"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"islet transplantation"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"islet isolation"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"islet purification"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"tissue loading"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"The purification step is one of the most important and difficult procedures in islet isolation for pancreatic islet transplantation. We previously reported that a purification method using large plastic bottles effectively achieved a high yield of islets from the porcine pancreas. In this study, we evaluated the impact of the timing of tissue loading on porcine islet purification using large plastic bottles. One method involved loading digested tissue after creating a continuous density gradient (tissue after gradient [TAG]). The other method involved loading digested tissue before creating a continuous density gradient (tissue before gradient [TBG]). There were no significant differences between TAG and TBG in terms of the islet yield, rates of viability and purity, score, and in the stimulation index after purification. Furthermore, there were no marked differences in the attainability or suitability of post-transplantation normoglycemia. Our study shows the equivalency of these two methods of islet purification.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"en","subitem_1522300316516":"SAGE Publishing"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"EISSN","subitem_1522646572813":"2155-1790"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"en","subitem_1522650091861":"Cell Medicine"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-06-29","bibliographicIssueDateType":"Issued"},"bibliographicVolumeNumber":"10"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_1522306287251":{"subitem_1522306382014":"DOI","subitem_1522306436033":"https://doi.org/10.1177/2155179018781343"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2023-03-08"}],"filename":"10.1177_2155179018781343.pdf","filesize":[{"value":"1.1 MB"}],"url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2019637/files/10.1177_2155179018781343.pdf"},"version_id":"48f298b9-c861-4d93-8d21-f892e7565182"}]},"item_title":"Comparison of Tissue Loading Before and After the Creation of a Continuous Density Gradient in Porcine Islet Purification","item_type_id":"59","owner":"8","path":["1642838338003","1642838407795"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2023-03-08"},"publish_date":"2023-03-08","publish_status":"0","recid":"2019637","relation_version_is_last":true,"title":["Comparison of Tissue Loading Before and After the Creation of a Continuous Density Gradient in Porcine Islet Purification"],"weko_creator_id":"8","weko_shared_id":-1},"updated":"2023-08-03T05:37:54.146863+00:00"}