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In Vitro Expression Analysis Reveals HML6-c14 to Be an Attractive Research Target
http://hdl.handle.net/20.500.12000/0002020826
http://hdl.handle.net/20.500.12000/0002020826c644bece-3eef-43c6-9285-751d7fc8d9f3
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
O_No13p1.pdf (4.3 MB)
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| Item type | 琉球大学リポジトリ登録用アイテムタイプ(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2024-11-22 | |||||||
| タイトル | ||||||||
| タイトル | In Vitro Expression Analysis Reveals HML6-c14 to Be an Attractive Research Target | |||||||
| 言語 | en | |||||||
| 作成者 |
Oda, Takaya
× Oda, Takaya
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| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| 権利情報 | ||||||||
| 言語 | en | |||||||
| 権利情報 | © 2023 by the author. | |||||||
| 権利情報 | ||||||||
| 言語 | en | |||||||
| 権利情報Resource | https://creativecommons.org/licenses/by/4.0/ | |||||||
| 権利情報 | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. | |||||||
| 主題 | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | human endogenous retrovirus | |||||||
| 主題 | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | placental expression | |||||||
| 主題 | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | long non-coding RNA | |||||||
| 主題 | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | nuclear retained transcript | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | HML6-c14, a long terminal repeat (LTR)-type retrotransposon identified by expressed sequence tag (EST) database screening, was found to undergo RNA processing resembling that of placental tissue by in vitro expression analysis. Previous in situ hybridization studies using normal placental tissue showed that the transcript remained in the nucleus. However, among the transcripts forcedly expressed in cultured cells, the transcript that retained the 3.3 kb intron was observed in the nucleus, and a part of the spliced transcript was observed outside the nucleus. To verify whether this cytoplasmic transcript could be translated, we examined the coding potential of the open reading frame (ORF), consisting of 109 codons on the spliced transcript, along with two other putative ORFs detected in the intronic region. As a result, none of the ORF-derived products could be detected by Western blotting as fusion proteins tagged with the FLAG epitope, suggesting that HML6-c14 belongs to a group of long non-coding RNA (lncRNA) genes. Promoter analysis of the upstream 6.4 kb genomic region also suggested that the 5′-LTR itself potentially retains high promoter activity. Despite losing the ability to produce functional proteins, HML6-c14 continues to retain its transcriptional ability while converting to an lncRNA gene, which is an interesting subject for future research. | |||||||
| 言語 | en | |||||||
| 出版者 | ||||||||
| 出版者 | MDPI | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 関連情報 | ||||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | https://doi.org/10.3390/biom13091378 | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | EISSN | |||||||
| 収録物識別子 | 2218-273X | |||||||
| 収録物名 | ||||||||
| 収録物名 | Biomolecules | |||||||
| 言語 | en | |||||||
| 書誌情報 |
巻 13, 号 9, p. 1-17, 発行日 2023-09-12 |
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