{"_buckets": {"deposit": "257736f6-5569-4abd-8cdb-316dbaa2c2a9"}, "_deposit": {"id": "2002628", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2002628"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02002628", "sets": ["1642837922702", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "\u30db\u30eb\u30b9\u30bf\u30a4\u30f3\u7a2e\u89aa\u5b50\u306e\u76f4\u8178\u4fbf\u7531\u6765\u5927\u8178\u83cc\u306b\u304a\u3051\u308b\u85ac\u5264\u8010\u6027", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "Antibiotic resistance in Escherichia coli isolates from rectal swab of Holstein cows and calves", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u5ca9\u5d0e, \u5065", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u9bab\u5cf6, \u597d\u7f8e", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u4e0b\u9580, \u5948\u6708", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u65e5\u8d8a, \u535a\u4fe1", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Iwasaki, Ken", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Samejima, Yoshimi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Shimojoh, Natsumi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Higoshi, Hironobu", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186609386": {"attribute_name": "Subject", "attribute_value_mlt": [{"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u5927\u8178\u83cc"}, {"subitem_1522299896455": "ja", 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"subitem_description_type": "Other"}, {"subitem_description": "A total of 252 Escherichia coli isolates were recovered from rectal swabs of two Holstein cows (designated cow A and B) on postpartum days 1 and 7 and the female calf of each (designated calf A and B, respectively) on postnatal days 1-7. Each isolate was tested for susceptibility to 7 antibiotics [ampicillin (ABPC), chloramphenicol (CP), chlortetracycline (CTC), kanamycin (KM), streptomycin (SM), nalidixic acid (NA), and sulfadimethoxine (SA)] and for prevalence of conjugative R-plasmids. The results of these studies are: 1. Resistance to at least one of antibiotics tested was found in 69% (173/252) of the E. coli isolates. The mean frequency of resistant isolates was: cow A, 20%; calf A, 61%; cow B, 80%; and calf B, 90%. These results show that calves are readily infected with resistant bacteria: on postnatal day 1, 47% of the E. coli isolates from calf A and 93% from calf B were antibiotic-resistant. Furthermore, the fraction of isolates that were antibiotic-resistant increased after postnatal day 3. 2. On postpartum day 7, isolates with resistance to only 4 of the antibiotics tested (ABPC, CTC, SM, and SA) were obtained from cow A and to only to 5 of the antibiotics (CP, CTC, SM, NA, and SA) from cow B. On postnatal day 1, both calves already had isolates with the same-antibiotic resistances as the corresponding cow. By postnatal day 2, isolates from calf A were also resistant to the other 3 antibiotics (CP, KM, and NA). Isolates resistant to one of the other antibiotics (ABPC) were found in calf B by postnatal day 5, but no isolate to the remaining antibiotic (KM) was found in calf B by postnatal day 7. 3. A number of antibiotic resistance patterns were found, ranging from isolates resistant to a single antibiotic to isolates resistant to multiple (i. e., 2-7) antibiotics. Isolates from calves had more resistance patterns than those from cows, and no isolates from cows were resistant to more than 4 antibiotics. On postnatal day 1, isolates from both calves already had different resistance patterns than those from the corresponding cow. Isolates with resistance to a single antibiotic were predominant during postnatal day 1-3. However, after postnatal day 3, isolates with resistance to multiple antibiotics were predominant. 4. The prevalence of a conjugative R-plasmid was investigated in 76 of the antibiotic-resistant isolates; 6 from cow A, 28 from calf A, 11 from cow B, and 31 from calf B. Conjugative R plasmid transfer was found in 4 of these isolates: a day 4 postnatal isolate from calf A with resistance to 1 antibiotic (ABPC), a day 6 postnatal isolate from calf A with resistance to 1 antibiotic (ABPC), a day 4 postnatal isolate from calf A with resistance to 3 antibiotics (ABPC-CP-CTC), and a day 3 postnatal isolate from calf B with resistance to 2 antibiotics (CTC-SA).", "subitem_description_type": "Other"}, {"subitem_description": "\u7d00\u8981\u8ad6\u6587", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/3595"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8\u5b66\u8853\u5831\u544a"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueNumber": "50", "bibliographicPageEnd": "59", "bibliographicPageStart": "53"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "KJ00004258133.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2002628/files/KJ00004258133.pdf"}, "version_id": "19687a59-93f4-4c34-a0b1-b55885c62655"}]}, "item_title": "\u30db\u30eb\u30b9\u30bf\u30a4\u30f3\u7a2e\u89aa\u5b50\u306e\u76f4\u8178\u4fbf\u7531\u6765\u5927\u8178\u83cc\u306b\u304a\u3051\u308b\u85ac\u5264\u8010\u6027", "item_type_id": "15", "owner": "1", "path": ["1642837922702", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/3595", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-02-14"}, "publish_date": "2008-02-14", "publish_status": "0", "recid": "2002628", "relation": {}, "relation_version_is_last": true, "title": ["\u30db\u30eb\u30b9\u30bf\u30a4\u30f3\u7a2e\u89aa\u5b50\u306e\u76f4\u8178\u4fbf\u7531\u6765\u5927\u8178\u83cc\u306b\u304a\u3051\u308b\u85ac\u5264\u8010\u6027"], "weko_shared_id": -1}