{"_buckets": {"deposit": "d18744c3-d324-4568-9570-392aa0ee9e77"}, "_deposit": {"id": "2002725", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2002725"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02002725", "sets": ["1642837920199", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "パインアップル葉における内生 ABA の同定, 定量法の確立およびその含量の日変化", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "Identification and quantification of endogenous ABA and the diurnal changes in ABA levels in pineapple plants", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "川満, 芳信", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "前田, 英樹", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Kawamitsu, Yoshinobu", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Maeda, Hideki", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186609386": {"attribute_name": "Subject", "attribute_value_mlt": [{"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "アブシジン酸"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "気孔開閉"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "CAM植物"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "パインアップル"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Abscisic acid"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Crassulacean Acid Metabolism"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Pineapple"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Stomata"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "パインアップルの葉の内生ABAの抽出, 精製法を確立し, サンプルを同定, 定量する方法を確立した.また, CAM植物であるパインアップル葉のABA含量に日変化があるか調べた。得られた結果は以下のように要約できる。1.パインアップルの葉におけるABAの抽出, 精製法が確立できた(第1図)。2.サンプルのABA-Meと, スタンダードの(±)-ABA-MeをGC/MSの2種類のイオン化法により同定した(第2,3図)。その結果, GCによるリテンションタイムが10.6minであり, ベースピーク(準安定イオン)がm/z190であることが確認された(第1表)。3.このm/z190のイオンをGC/SIM法により測定し(第4図), スタンダードの濃度とm/z190によるマスクロマトグラフのリテンションタイム10.6におけるピーク面積が比例することが確認できた。従って, スタンダードの濃度とピーク面積により検量線を作成した。4.本精製法における内部標準物質(±)-ABAの回収率は67.5%であり, ABAが精製過程である程度消失していることが認められた(第2表)。5.パインアップルの葉におけるABA含量を測定した結果, 明かな日変動が見られた(第3表)。これは気孔伝導度の日変化とほぼ対応していた。以上より, CAM植物の気孔の日変化には, ABAも関与していることが示唆された。", "subitem_description_type": "Other"}, {"subitem_description": "We developed the assay method for the abscisic acid (ABA) content by means with GC/MS and applied the method to measure the diurnal changes in pineapple, crassulacean acid metabolism plant. The stomata of pineapple leaves opened during night time (phase-1), closed during day time (phase-3), and reopened during late afternoon (phase-4). The ABA levels in phase-3 were increased as compared with in phase-1 and phase-4. It was suggested that the ABA content was in part contributing the stomatal control in crassulacean acid metabolism plants.", "subitem_description_type": "Other"}, {"subitem_description": "紀要論文", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "琉球大学農学部"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/3696"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "琉球大学農学部学術報告"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueNumber": "44", "bibliographicPageEnd": "89", "bibliographicPageStart": "81"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "KJ00000161817.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2002725/files/KJ00000161817.pdf"}, "version_id": "9418011c-b262-4430-aad2-2c977d40269e"}]}, "item_title": "パインアップル葉における内生 ABA の同定, 定量法の確立およびその含量の日変化", "item_type_id": "15", "owner": "1", "path": ["1642837920199", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/3696", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-02-14"}, "publish_date": "2008-02-14", "publish_status": "0", "recid": "2002725", "relation": {}, "relation_version_is_last": true, "title": ["パインアップル葉における内生 ABA の同定, 定量法の確立およびその含量の日変化"], "weko_shared_id": -1}
パインアップル葉における内生 ABA の同定, 定量法の確立およびその含量の日変化
http://hdl.handle.net/20.500.12000/3696
http://hdl.handle.net/20.500.12000/3696