{"_buckets": {"deposit": "211a147b-5e4c-47d2-9e46-ca9911296a8f"}, "_deposit": {"id": "2002816", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2002816"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02002816", "sets": ["1642837918448", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "沖縄のウリ科植物に発生する Potyvirus に関する研究 : 第 2 報 スズメウリ斑紋ウイルスの性質(生物生産学科)", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "Studies on Potyvirus Parasite on Cucurbitaceae in Okinawa : 2. Some properties of Melothria Mottle Virus (Department of Bioproduction)", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "与那覇, 哲義", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "永田, めぐみ", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "河野, 伸二", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "田盛, 正雄", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Yonaha, Tetsuyoshi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Nagata, Megumi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Kawano, Shinji", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Tamori, Masao", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "1. 1991年2月に那覇市首里石嶺町で採集したクロミノオキナワスズメウリ(Melothria liukiuensis Nakai)の退緑斑紋症状を示した病葉から分離したウイルスの同定試験を行った。2. 9科42種植物に汁液接種したところ,ウリ科12種,アカザ科4種,N.benthamiara,シロゴマ,センニチコウおよびツルナの6科20種の感染が認められた。ウイルスは容易に汁液伝染し,モモアカアブラムシにより非永続的に伝搬された。3. ウイルスの耐熱性(10分)は60\u0026acd;65℃,耐希釈性は(10)^\u003c-3\u003e\u0026acd;(10)^\u003c-4\u003eおよび耐保存性(20℃)は8\u0026acd;10日にあった。セイヨウカボチャを検定植物に用いてWMV-2,ZYMV, PRSV-WおよびTrMVに対するMeMVの干渉効果を検定した結果,どちらのウイルスとも干渉効果は認められなかった。4. 2%リンタングステン酸で染色したDip法試料中のウイルス粒子は725×12nmの湾曲したひも状粒子が観察された。5. 免疫電顕法および寒天ゲル内拡散法による血清反応を調べたところ,MeMVはWMV-2,ZYMV, PRSV-WおよびTrMVのいずれのウイルスとも血清的関係は認められなかった。6. 本報のウイルスは新Potyvirusと判断されたのでスズメウリ斑紋ウイルス(Melothria mottle virus : MeMV)と命名した。", "subitem_description_type": "Other"}, {"subitem_description": "A virus was isolated from a wild Cucurbitaceae (Melothria liukiuensis Nakai) plant showing faint mottle collected in Naha, Okinawa prefecture. Identification of the virus was based on host range, aphid transmission, physical properties. cross propection, particle morphology and serology. The host range of the virus was moderately wide, 20 species in the families Cucurbitaceae, Solanaceae, Pedaleaceae Aizoaceae, Chenopodiaceae and Amaranthaceae among 42 species belonging to 9 families tested. The virus was transmitted by aphid (Myzus persicae) in the non-persistent manner. The thermal inactivation was between 60\u0026acd;65℃, dilution end point was between (10)^\u003c-3\u003e\u0026acd;(10)^\u003c-4\u003e and longevity in vitro was between 8\u0026acd;10 days at 20℃ in crude sap of diseased Nicotiana benthamiana. No cross protection was detected between the virus and watermelon mosaic virus-2,zucchini yellow mosaic virus papaya ringspot virus-W and trichosanthus mottle virws as test plant of Cucurbita maxima used. The virus particles were flexuous filamentous, about 725×12nm in leaf dip preparation. The titer of antiserum to the virus was 1 : 256 in the ring interface tests. The antiserum reacted with homologous antigen, but not reacted with WMV-2 and ZYMV in immuno-electron microscopy and in agar gel diffusion tests. The virus did not reacted with antisera against WMV-2,ZYMV, papaya ringspot virus-W and trichosanthus mottle virus in immuno-electron microscopy. Based on the results, the virus was identified as Melothria motte virus (MeMV), a new potyvirus.", "subitem_description_type": "Other"}, {"subitem_description": "紀要論文", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "琉球大学農学部"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/3787"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "琉球大学農学部学術報告"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1993-12-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "40", "bibliographicPageEnd": "19", "bibliographicPageStart": "9"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "KJ00000161716.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2002816/files/KJ00000161716.pdf"}, "version_id": "546dfa4e-78a0-4d6c-b1e8-fe93533a0373"}]}, "item_title": "沖縄のウリ科植物に発生する Potyvirus に関する研究 : 第 2 報 スズメウリ斑紋ウイルスの性質(生物生産学科)", "item_type_id": "15", "owner": "1", "path": ["1642837918448", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/3787", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-02-14"}, "publish_date": "2008-02-14", "publish_status": "0", "recid": "2002816", "relation": {}, "relation_version_is_last": true, "title": ["沖縄のウリ科植物に発生する Potyvirus に関する研究 : 第 2 報 スズメウリ斑紋ウイルスの性質(生物生産学科)"], "weko_shared_id": -1}