{"_buckets": {"deposit": "f83d5bc8-e5d1-4ffc-946e-7c4a8ce645eb"}, "_deposit": {"id": "2002903", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2002903"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02002903", "sets": ["1642837916513", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "\u6210\u719f\u30b5\u30c8\u30a6\u30ad\u30d3\u830e\u68a2\u982d\u90e8\u306e\u03b1-\u30a2\u30df\u30e9\u30fc\u30bc\u306b\u95a2\u3059\u308b\u7814\u7a76(\u8fb2\u82b8\u5316\u5b66\u79d1)", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "Studies on \u03b1-Amylase from Top Part of Mature Sugar Cane Stalks (Department of Agricultural Chemistry)", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u77e5\u5ff5, \u529f", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u5bae\u57ce, \u5927", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u4f0a\u85e4, \u656c\u53f2", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u9688\u5143, \u4fca\u548c", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u5c4b, \u5b8f\u5178", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Chinen, Isao", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Miyagi, Masaru", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Ito, Takafumi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Kumamoto, Tosikazu", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Oku, Hirosuke", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186626617": {"attribute_name": 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"subitem_description_type": "Other"}, {"subitem_description": "In the first place two types of starch-hydrolyzing enzyme were found to be in top part of mature sugar cane stalk. Because one of them was adsorbed to an \u03b1-cyclodextrin-Sepharose column, the other was passed through it. In this paper the former was purified and various properties of it were studied. Purification was carried out by affinity chromatography on epoxy activited Sepharose 6B substituted with \u03b1-cyclodextrin and then by gel filtration on Sephadex G-100. As a result, two kinds of amylase showing affinity to \u03b1-cyclodextrin-Sepharose existed in the fraction. One of them had high activity and showed homogeneity by disk gel electrophoresis. But the other had low activity and was obtained in very small amounts. So further studies of the former were achieved. The purity was 66,477 times and the recovery was 75%. The molecular weight was estimated to be about 69,000 by disk gel electrophoresis. The optimum temperature and pH volues were 65\u2103 and 5.0,respectively. The Km and Vmax values for soluble starch were 2.15mg/ml and 146 units/mg/min. The amylase indicated remarkable thermostability when 1mM calcium chloride was present. In other words, it was stable at 55\u2103 for one hour. The enzyme cleaved considerably soluble starch, amylopectin, amylose, dextrin, and glycogen, but wheat starch, rice starch, cassava starch, sweet potato starch, potato starch, in less extent than the formers. Oligosaccharide composition of products from theses starches was determined by high performance liquid chromatography. No difference in tendency of the composition was detected among the starches. But maltoheptaose (G_7) was mainly produced from each starch. Maltooctaose (G_8), maltohexaose (G_6) maltopentaose (G_5), maltotetraose (G_4), maltotriose (G_3) and maltose (G_2) were also formed in less content than the former. Optical rotation of decomposition products from soluble starch was determined, but the result was not clear. But the enzyme was adsorbed to \u03b1-cylodextrin Sepharose, hydrolyzed synthetic substrate (amylose azure), which has been used as a substrate of \u03b1-amylase, and was inhibited by \u03b1-amylase inhibitor from Triticum aestivlm, therefore it was decided to be \u03b1-amylase producing \u03b1-type oligosaccharides from various starches. Two kinds of \u03b1-amylase was found to be in this fraction in the purification as stated above. One of them showing minor activity was found to produce only maltose (G_2) from soluble starch by HPLC.", "subitem_description_type": "Other"}, {"subitem_description": "\u7d00\u8981\u8ad6\u6587", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/3874"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8\u5b66\u8853\u5831\u544a"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueNumber": "36", "bibliographicPageEnd": "49", "bibliographicPageStart": "33"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "KJ00000161616.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2002903/files/KJ00000161616.pdf"}, "version_id": "77960d0c-7d63-4e1c-96d6-8c81d624427f"}]}, "item_title": "\u6210\u719f\u30b5\u30c8\u30a6\u30ad\u30d3\u830e\u68a2\u982d\u90e8\u306e\u03b1-\u30a2\u30df\u30e9\u30fc\u30bc\u306b\u95a2\u3059\u308b\u7814\u7a76(\u8fb2\u82b8\u5316\u5b66\u79d1)", "item_type_id": "15", "owner": "1", "path": ["1642837916513", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/3874", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-02-14"}, "publish_date": "2008-02-14", "publish_status": "0", "recid": "2002903", "relation": {}, "relation_version_is_last": true, "title": ["\u6210\u719f\u30b5\u30c8\u30a6\u30ad\u30d3\u830e\u68a2\u982d\u90e8\u306e\u03b1-\u30a2\u30df\u30e9\u30fc\u30bc\u306b\u95a2\u3059\u308b\u7814\u7a76(\u8fb2\u82b8\u5316\u5b66\u79d1)"], "weko_shared_id": -1}