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No. 430\u306e\u751f\u7523\u3059\u308b\u30a2\u30e9\u30d3\u30ca\u30f3\u5206\u89e3\u9175\u7d20\u306e\u7cbe\u88fd\u3092\u884c\u3044, \u305d\u306e\u9175\u7d20\u5316\u5b66\u7684\u6027\u8cea\u3092\u691c\u8a0e\u3057\u305f\u3002\u9175\u7d20\u306e\u7cbe\u88fd\u306f, \u4f9b\u8a66\u83cc\u682a\u306e\u57f9\u990a\u6ffe\u6db2\u304b\u3089\u786b\u9178\u30a2\u30f3\u30e2\u30cb\u30a6\u30e0\u5206\u753b, DEAE-\u30bb\u30eb\u30ed\u30fc\u30b9, 2\u56de\u3081\u306eDEAE-\u30bb\u30eb\u30ed\u30fc\u30b9, QAE-\u30bb\u30d5\u30a1\u30c7\u30c3\u30af\u30b9A-50,DEAE-\u30c8\u30fc\u30e8\u30fc\u30d1\u30fc\u30eb650S\u53ca\u3073\u30bb\u30d5\u30a1\u30c7\u30c3\u30af\u30b9G-75\u306a\u3069\u306e\u30ab\u30e9\u30e0\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u3092\u7d44\u307f\u5408\u308f\u305b\u3066\u884c\u306a\u3063\u305f\u3002\u7cbe\u88fd\u9175\u7d20\u306f\u30c7\u30a3\u30b9\u30af\u96fb\u6c17\u6cf3\u52d5\u7684\u306b\u9ad8\u5ea6\u306b\u7cbe\u88fd\u3055\u308c\u3066\u3044\u305f\u3002\u7cbe\u88fd\u9175\u7d20\u306e\u53cd\u5fdc\u81f3\u9069pH\u306f7.0,\u53cd\u5fdc\u81f3\u9069\u6e29\u5ea6\u306f40\u2103\u3067\u3042\u3063\u305f\u3002\u672c\u9175\u7d20\u53cd\u5fdc\u306fCu^\u003c2+\u003e, Cd^\u003c2+\u003e, \u03b1, \u03b1\u0027-\u30b8\u30d4\u30ea\u30b8\u30eb, \u6c83\u5ea6\u9162\u9178, \u30d1\u30e9\u30af\u30ed\u30ed\u30de\u30fc\u30ad\u30e5\u30ea\u30fc\u30d9\u30f3\u30be\u30a8\u30fc\u30c8\u306b\u3088\u308a\u90e8\u5206\u7684\u306b\u963b\u5bb3\u3092\u53d7\u3051\u305f\u3002\u7cbe\u88fd\u9175\u7d20\u306f\u30d3\u30fc\u30c8\u30a2\u30e9\u30d3\u30ca\u30f3, \u30a2\u30e9\u30d3\u30ce\u30ac\u30e9\u30af\u30bf\u30f3\u3084\u30d1\u30e9\u30fc\u30cb\u30c8\u30ed\u30d5\u30a7\u30cb\u30eb\u30a2\u30e9\u30d3\u30ce\u30d5\u30e9\u30ce\u30b7\u30c9\u306b\u4f5c\u7528\u3057\u305f\u3002\u53cd\u5fdc\u751f\u6210\u7269\u306fL-\u30a2\u30e9\u30d3\u30ce\u30fc\u30b9\u306e\u307f\u304c\u691c\u51fa\u3055\u308c\u305f\u3002\u672c\u9175\u7d20\u306f\u03b1-L-\u30a2\u30e9\u30d3\u30ce\u30d5\u30e9\u30ce\u30b7\u30c0\u30fc\u30bc\u3068\u3057\u3066\u7279\u5fb4\u3065\u3051\u3089\u308c\u305f\u3002\u672c\u9175\u7d20\u3092\u7528\u3044\u308b\u3053\u3068\u306b\u3088\u308a, \u3055\u3068\u3046\u304d\u3073\u30d0\u30ac\u30b9\u304b\u3089\u9ad8\u7d14\u5ea6\u306e\u30ad\u30b7\u30e9\u30f3\u304c\u5f97\u3089\u308c\u305f\u3002", "subitem_description_type": "Other"}, {"subitem_description": "This study was carried out for an effective utilization of agricultural wastes by microbial enzymes. 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  1. 紀要論文
  2. 琉球大学農学部学術報告
  3. 35号
  1. 部局別インデックス
  2. 農学部

Bacillus sp. No. 430 のα-L-アラビノフラノシダーゼの精製と性質(農芸化学科)

http://hdl.handle.net/20.500.12000/3891
http://hdl.handle.net/20.500.12000/3891
988b9ddf-96a9-42aa-9f64-fb7c2b116231
名前 / ファイル ライセンス アクション
KJ00000161594.pdf KJ00000161594.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2008-02-14
タイトル
タイトル Bacillus sp. No. 430 のα-L-アラビノフラノシダーゼの精製と性質(農芸化学科)
言語 ja
作成者 安田, 正昭

× 安田, 正昭

ja 安田, 正昭

徳里, 政丈

× 徳里, 政丈

ja 徳里, 政丈

Yasuda, Masaaki

× Yasuda, Masaaki

en Yasuda, Masaaki

Tokuzato, Masatake

× Tokuzato, Masatake

en Tokuzato, Masatake

アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
内容記述
内容記述タイプ Other
内容記述 Bacillus sp. No. 430の生産するアラビナン分解酵素の精製を行い, その酵素化学的性質を検討した。酵素の精製は, 供試菌株の培養濾液から硫酸アンモニウム分画, DEAE-セルロース, 2回めのDEAE-セルロース, QAE-セファデックスA-50,DEAE-トーヨーパール650S及びセファデックスG-75などのカラムクロマトグラフィーを組み合わせて行なった。精製酵素はディスク電気泳動的に高度に精製されていた。精製酵素の反応至適pHは7.0,反応至適温度は40℃であった。本酵素反応はCu^<2+>, Cd^<2+>, α, α'-ジピリジル, 沃度酢酸, パラクロロマーキュリーベンゾエートにより部分的に阻害を受けた。精製酵素はビートアラビナン, アラビノガラクタンやパラーニトロフェニルアラビノフラノシドに作用した。反応生成物はL-アラビノースのみが検出された。本酵素はα-L-アラビノフラノシダーゼとして特徴づけられた。本酵素を用いることにより, さとうきびバガスから高純度のキシランが得られた。
内容記述タイプ Other
内容記述 This study was carried out for an effective utilization of agricultural wastes by microbial enzymes. In order to liberate arabinose from arabinose-containing polysaccharides such as arabinan, arabinoxylan, and arabinogalactan, α-L-arabinofuranosidase was investigated. An α-L-arabinofuranosidase II was purified from the culture fluid of Bacillus sp. No. 430,which was isolated from the soil of sugar cane fields. The process of purification was as follows; salting out by ammonium sulfate, first DEAE-cellulose, second DEAE-cellulose, QAE-Sephadex A-50,DEAE-Toyopearl 650 S, and Sephadex G-75 column chromatography. The enzyme was highly purified by the method of disc gel electrophoresis. The purified enzyme had the maximum reactivity at pH 7.0 and 40℃. The enzyme was partially inhibited by Cu^<2+>, Cd^<2+>, α, α′-dipyridyl, iodoacetic acid, and p-chloromercuribenzoic acid. The highly purified enzyme was confirmed to liberate L-arabinose from beet arabinan, arabinogalactan, arabinoxylan, and p-nitrophenyl α-L-arabinofuranoside. The reaction product was paper chromatographically demonstrated to be L-arabinose only. The purified enzyme was inactive for p-nitrophenyl α-, β-D-galactopyranosides and p-nitrophenyl β-D-xylopyranoside. And thus, it was characterized as α-L-arabinofuranosidase which was exo-arabinanase. High purity of xylan from sugar cane bagasse or galactans from coffee bean and soybean was obtained with the enzyme.
内容記述タイプ Other
内容記述 紀要論文
出版者
言語 ja
出版者 琉球大学農学部
言語
言語 eng
資源タイプ
資源タイプ departmental bulletin paper
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
識別子
識別子 http://hdl.handle.net/20.500.12000/3891
識別子タイプ HDL
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0370-4246
収録物識別子タイプ NCID
収録物識別子 AN00250548
収録物名
言語 ja
収録物名 琉球大学農学部学術報告
書誌情報
号 35, p. 53-62
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