{"_buckets": {"deposit": "549714b2-3aec-4fe5-9ce9-11cd19bb473a"}, "_deposit": {"id": "2002920", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2002920"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02002920", "sets": ["1642837915948", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "Bacillus sp. No. 430 \u306e\u03b1-L-\u30a2\u30e9\u30d3\u30ce\u30d5\u30e9\u30ce\u30b7\u30c0\u30fc\u30bc\u306e\u7cbe\u88fd\u3068\u6027\u8cea(\u8fb2\u82b8\u5316\u5b66\u79d1)", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "Purification and Properties of \u03b1-L-Arabinofuranosidase II from Bacillus sp. No. 430 (Department of Agricultural Chemistry)", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u5b89\u7530, \u6b63\u662d", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u5fb3\u91cc, \u653f\u4e08", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Yasuda, Masaaki", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Tokuzato, Masatake", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "Bacillus sp. No. 430\u306e\u751f\u7523\u3059\u308b\u30a2\u30e9\u30d3\u30ca\u30f3\u5206\u89e3\u9175\u7d20\u306e\u7cbe\u88fd\u3092\u884c\u3044, \u305d\u306e\u9175\u7d20\u5316\u5b66\u7684\u6027\u8cea\u3092\u691c\u8a0e\u3057\u305f\u3002\u9175\u7d20\u306e\u7cbe\u88fd\u306f, \u4f9b\u8a66\u83cc\u682a\u306e\u57f9\u990a\u6ffe\u6db2\u304b\u3089\u786b\u9178\u30a2\u30f3\u30e2\u30cb\u30a6\u30e0\u5206\u753b, DEAE-\u30bb\u30eb\u30ed\u30fc\u30b9, 2\u56de\u3081\u306eDEAE-\u30bb\u30eb\u30ed\u30fc\u30b9, QAE-\u30bb\u30d5\u30a1\u30c7\u30c3\u30af\u30b9A-50,DEAE-\u30c8\u30fc\u30e8\u30fc\u30d1\u30fc\u30eb650S\u53ca\u3073\u30bb\u30d5\u30a1\u30c7\u30c3\u30af\u30b9G-75\u306a\u3069\u306e\u30ab\u30e9\u30e0\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u3092\u7d44\u307f\u5408\u308f\u305b\u3066\u884c\u306a\u3063\u305f\u3002\u7cbe\u88fd\u9175\u7d20\u306f\u30c7\u30a3\u30b9\u30af\u96fb\u6c17\u6cf3\u52d5\u7684\u306b\u9ad8\u5ea6\u306b\u7cbe\u88fd\u3055\u308c\u3066\u3044\u305f\u3002\u7cbe\u88fd\u9175\u7d20\u306e\u53cd\u5fdc\u81f3\u9069pH\u306f7.0,\u53cd\u5fdc\u81f3\u9069\u6e29\u5ea6\u306f40\u2103\u3067\u3042\u3063\u305f\u3002\u672c\u9175\u7d20\u53cd\u5fdc\u306fCu^\u003c2+\u003e, Cd^\u003c2+\u003e, \u03b1, \u03b1\u0027-\u30b8\u30d4\u30ea\u30b8\u30eb, \u6c83\u5ea6\u9162\u9178, \u30d1\u30e9\u30af\u30ed\u30ed\u30de\u30fc\u30ad\u30e5\u30ea\u30fc\u30d9\u30f3\u30be\u30a8\u30fc\u30c8\u306b\u3088\u308a\u90e8\u5206\u7684\u306b\u963b\u5bb3\u3092\u53d7\u3051\u305f\u3002\u7cbe\u88fd\u9175\u7d20\u306f\u30d3\u30fc\u30c8\u30a2\u30e9\u30d3\u30ca\u30f3, \u30a2\u30e9\u30d3\u30ce\u30ac\u30e9\u30af\u30bf\u30f3\u3084\u30d1\u30e9\u30fc\u30cb\u30c8\u30ed\u30d5\u30a7\u30cb\u30eb\u30a2\u30e9\u30d3\u30ce\u30d5\u30e9\u30ce\u30b7\u30c9\u306b\u4f5c\u7528\u3057\u305f\u3002\u53cd\u5fdc\u751f\u6210\u7269\u306fL-\u30a2\u30e9\u30d3\u30ce\u30fc\u30b9\u306e\u307f\u304c\u691c\u51fa\u3055\u308c\u305f\u3002\u672c\u9175\u7d20\u306f\u03b1-L-\u30a2\u30e9\u30d3\u30ce\u30d5\u30e9\u30ce\u30b7\u30c0\u30fc\u30bc\u3068\u3057\u3066\u7279\u5fb4\u3065\u3051\u3089\u308c\u305f\u3002\u672c\u9175\u7d20\u3092\u7528\u3044\u308b\u3053\u3068\u306b\u3088\u308a, \u3055\u3068\u3046\u304d\u3073\u30d0\u30ac\u30b9\u304b\u3089\u9ad8\u7d14\u5ea6\u306e\u30ad\u30b7\u30e9\u30f3\u304c\u5f97\u3089\u308c\u305f\u3002", "subitem_description_type": "Other"}, {"subitem_description": "This study was carried out for an effective utilization of agricultural wastes by microbial enzymes. In order to liberate arabinose from arabinose-containing polysaccharides such as arabinan, arabinoxylan, and arabinogalactan, \u03b1-L-arabinofuranosidase was investigated. An \u03b1-L-arabinofuranosidase II was purified from the culture fluid of Bacillus sp. No. 430,which was isolated from the soil of sugar cane fields. The process of purification was as follows; salting out by ammonium sulfate, first DEAE-cellulose, second DEAE-cellulose, QAE-Sephadex A-50,DEAE-Toyopearl 650 S, and Sephadex G-75 column chromatography. The enzyme was highly purified by the method of disc gel electrophoresis. The purified enzyme had the maximum reactivity at pH 7.0 and 40\u2103. The enzyme was partially inhibited by Cu^\u003c2+\u003e, Cd^\u003c2+\u003e, \u03b1, \u03b1\u2032-dipyridyl, iodoacetic acid, and p-chloromercuribenzoic acid. The highly purified enzyme was confirmed to liberate L-arabinose from beet arabinan, arabinogalactan, arabinoxylan, and p-nitrophenyl \u03b1-L-arabinofuranoside. The reaction product was paper chromatographically demonstrated to be L-arabinose only. The purified enzyme was inactive for p-nitrophenyl \u03b1-, \u03b2-D-galactopyranosides and p-nitrophenyl \u03b2-D-xylopyranoside. And thus, it was characterized as \u03b1-L-arabinofuranosidase which was exo-arabinanase. High purity of xylan from sugar cane bagasse or galactans from coffee bean and soybean was obtained with the enzyme.", "subitem_description_type": "Other"}, {"subitem_description": "\u7d00\u8981\u8ad6\u6587", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "eng"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/3891"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8\u5b66\u8853\u5831\u544a"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueNumber": "35", "bibliographicPageEnd": "62", "bibliographicPageStart": "53"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "KJ00000161594.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2002920/files/KJ00000161594.pdf"}, "version_id": "5bf51460-67b1-44fe-b468-3a843c1ca090"}]}, "item_title": "Bacillus sp. No. 430 \u306e\u03b1-L-\u30a2\u30e9\u30d3\u30ce\u30d5\u30e9\u30ce\u30b7\u30c0\u30fc\u30bc\u306e\u7cbe\u88fd\u3068\u6027\u8cea(\u8fb2\u82b8\u5316\u5b66\u79d1)", "item_type_id": "15", "owner": "1", "path": ["1642837915948", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/3891", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-02-14"}, "publish_date": "2008-02-14", "publish_status": "0", "recid": "2002920", "relation": {}, "relation_version_is_last": true, "title": ["Bacillus sp. No. 430 \u306e\u03b1-L-\u30a2\u30e9\u30d3\u30ce\u30d5\u30e9\u30ce\u30b7\u30c0\u30fc\u30bc\u306e\u7cbe\u88fd\u3068\u6027\u8cea(\u8fb2\u82b8\u5316\u5b66\u79d1)"], "weko_shared_id": -1}