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  1. 紀要論文
  2. 琉球大学農学部学術報告
  3. 31号
  1. 部局別インデックス
  2. 農学部

蔗糖を結合した水飴の製造(農芸化学科)

http://hdl.handle.net/20.500.12000/3962
http://hdl.handle.net/20.500.12000/3962
af054828-3300-46ff-83fe-854ac4020b8b
名前 / ファイル ライセンス アクション
KJ00000162202.pdf KJ00000162202.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2008-02-14
タイトル
タイトル 蔗糖を結合した水飴の製造(農芸化学科)
言語 ja
作成者 仲村, 実久

× 仲村, 実久

ja 仲村, 実久

角, 満雄

× 角, 満雄

ja 角, 満雄

田幸, 正邦

× 田幸, 正邦

ja 田幸, 正邦

Nakamura, Sanehisa

× Nakamura, Sanehisa

en Nakamura, Sanehisa

Sumi, Mitsuo

× Sumi, Mitsuo

en Sumi, Mitsuo

Tako, Masakuni

× Tako, Masakuni

en Tako, Masakuni

アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
内容記述
内容記述タイプ Other
内容記述 B.maceransの分泌するCGT-aseを用い, 蔗糖を受容体として, 糖転移作用を調べた。まず, 澱粉培地で培養した遠心分離上清液を粗酵素とし, 0.25飽和硫安分画, 澱粉吸着, 溶出して精製し, 60倍の比活性の酵素液を調製した。次にこの酵素を用いて, 澱粉, 蔗糖混合液に作用させた。蔗糖添加量を増大するに従って, 澱粉の分解速度が増大した。相対粘度は, 澱粉10および20%とも反応初期に急激に減少し, 20%反応液ではその後増大する傾向が見られた。PPCによりグルコース, マルトースおよびマルトトリオースは検出されず, Rf値の異なったオリゴ糖が検出され, これらはケートース反応に陽性であることから, 蔗糖を含むオリゴ糖であることが認められた。また, 文献値より, 生成したオリゴ糖はG_2F(maltopyranosyl-fructfuranoside), G_3F(maltotripyranosyl-fructofuranoside), G_4F(maltotetrapyranosyl-fructofuranoside)等であると推定した。
内容記述タイプ Other
内容記述 Cyclodextrin glycosyltransferase was prepared from the culture fluid of Bacillus macerans and was purified partially by the fractionation by addition of ammoniun sulphate, and the adsorption method on starch. In order to prepare sucrose-bound syrup, cyclodextrin glyco-syltransferase was allowed to react with a mixed solution of soluble starch and sucrose. In the initial stage of the reaction, specific viscosity of a reaction mixture decreased remarkably and, then it increased at the starch concentration of 20% in both of 10 and 20% sucrose-mixed solution. Cyclodextrin production decreased with an increase in sucrose. The final product separated by paperchrom-atography was identified as maltooligosaccharides substituting sucrose at reducing end.
内容記述タイプ Other
内容記述 紀要論文
出版者
言語 ja
出版者 琉球大学農学部
言語
言語 jpn
資源タイプ
資源タイプ departmental bulletin paper
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
識別子
識別子 http://hdl.handle.net/20.500.12000/3962
識別子タイプ HDL
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0370-4246
収録物識別子タイプ NCID
収録物識別子 AN00250548
収録物名
言語 ja
収録物名 琉球大学農学部学術報告
書誌情報
号 31, p. 43-50, 発行日 1984-11-19
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