{"_buckets": {"deposit": "06625426-ce43-42aa-b64b-52d0e2a3ae03"}, "_deposit": {"id": "2003159", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2003159"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02003159", "sets": ["1642837911830", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "\u7518\u8517\u830e\u306e\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc\u3068\u03b1-\u30ac\u30e9\u30af\u30c8\u30b7\u30c0\u30fc\u30bc\u306b\u95a2\u3059\u308b\u7814\u7a76(\u8fb2\u82b8\u5316\u5b66\u79d1)", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "Studies on acid invertase and \u03b1-galactosidase of sugar cane stalk (Department of Agricultural Chemistry)", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u77e5\u5ff5, \u529f", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u677e\u4e0b, \u6d0b\u4e00", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u798f\u7530, \u4e98\u535a", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Chinen, Isao", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Matsushita, Yoichi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Fukuda, Nobuhiro", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "\u7518\u8517\u830e\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc\u6d3b\u6027\u306f, \u8517\u7cd6\u6fc3\u5ea6\u304c\u4f4e\u304f, \u9084\u5143\u7cd6\u91cf\u304c\u6bd4\u8f03\u7684\u9ad8\u304f, \u830e\u306e\u592a\u3055, \u9577\u3055\u304c\u5341\u5206\u751f\u80b2\u3057\u305f\u7b2c4\u7bc0\u9593\u3067\u9ad8\u304b\u3063\u305f\u3002\u7518\u8517\u830e\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc\u3092\u8517\u6c41\u3088\u308a, 60%\u786b\u5b89\u98fd\u548c\u6c88\u6fb1\u6cd5, DEAE\u30bb\u30eb\u30ed\u30fc\u30b9\u304a\u3088\u3073\u30bb\u30d5\u30a1\u30c7\u30a3\u30af\u30b9G-200\u30ab\u30e9\u30e0\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u306b\u3088\u308a, \u7d042,000\u500d\u306b\u7cbe\u88fd\u3057\u305f\u3002\u3053\u306e\u7cbe\u88fd\u5206\u753b\u306f, \u96fb\u6c17\u6cf3\u52d5\u7684\u306b2\u3064\u306e\u30bf\u30f3\u30d1\u30af\u30d0\u30f3\u30c9\u304c\u307f\u3089\u308c\u305f\u3002\u307e\u305f\u3053\u306e\u30d0\u30f3\u30c9\u306f, \u904e\u30e8\u30a6\u7d20\u9178-Schiff\u8a66\u85ac\u3067\u3082\u67d3\u8272\u3055\u308c\u305f\u3002\u3053\u306e\u7cbe\u88fd\u9175\u7d20\u5206\u753b\u306f, \u30b7\u30e5\u30af\u30ed\u30fc\u30b9, \u30e9\u30d5\u30a3\u30ce\u30fc\u30b9, p-Nitrophenyl-\u03b1-D-galactopyranoside\u306b\u5bfe\u3057, \u5f37\u3044\u6c34\u89e3\u6d3b\u6027\u304c\u898b\u3089\u308c\u305f\u3002p-nitrophenyl-2-acetamido-2-deoxy-\u03b2-D-glucopy-ranoside\u304a\u3088\u3073p-nitrophenyl-2-acetamido-2-deoxy-\u03b2-D-galactopyranoside\u306b\u5bfe\u3057\u3066\u3082\u308f\u305a\u304b\u306a\u304c\u3089\u6c34\u89e3\u6d3b\u6027\u304c\u898b\u3089\u308c\u305f\u3002\u3053\u306e\u9175\u7d20\u5206\u753b\u306e\u71b1\u5b89\u5b9a\u6027\u3092\u8abf\u3079\u305f\u7d50\u679c, \u30b7\u30e5\u30af\u30ed\u30fc\u30b9\u3092\u57fa\u8cea\u3068\u3057\u3066\u7528\u3044\u3066\u6d3b\u6027\u3092\u6e2c\u5b9a\u3057\u305f\u5834\u5408, 45\u2103\u4ee5\u4e0a\u3067\u306f, \u4e0d\u5b89\u5b9a\u3067\u3042\u3063\u305f\u3002\u30e9\u30d5\u30a3\u30ce\u30fc\u30b9\u308b\u57fa\u8cea\u3068\u3057\u3066\u7528\u3044\u305f\u5834\u5408\u306f, 55\u2103\u4ee5\u4e0a\u3067\u4e0d\u5b89\u5b9a\u3067\u3042\u3063\u305f\u3002\u3053\u306e\u5206\u753b\u3092\u30e9\u30d5\u30a3\u30ce\u30fc\u30b9\u306b\u53cd\u5fdc(45\u2103, 30\u5206\u9593)\u3055\u305b, \u3053\u306e\u53cd\u5fdc\u6db2\u3092\u30da\u30fc\u30d1\u30fc\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u306b\u304b\u3051\u305f\u7d50\u679c, \u30e9\u30a3\u30d5\u30a3\u30ce\u30fc\u30b9, \u30ac\u30e9\u30af\u30c8\u30fc\u30b9, \u30b7\u30e5\u30af\u30ed\u30fc\u30b9\u306e\u30b9\u30dd\u30c3\u30c8\u3068\u30d5\u30e9\u30af\u30c8\u30fc\u30b9\u306e\u30b9\u30dd\u30c3\u30c8\u304c\u307f\u3089\u308c\u305f\u3002\u3053\u306e\u9175\u7d20\u5206\u753b\u306b\u306f, \u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc\u4ee5\u5916\u306b\u03b1-\u30ac\u30e9\u30af\u30c8\u30b7\u30c0\u30fc\u30bc\u304c\u5b58\u5728\u3059\u308b\u3053\u3068\u3092\u793a\u5506\u3057\u3066\u3044\u308b\u3002\u3053\u306e\u03b1-\u30ac\u30e9\u30af\u30c8\u30b7\u30c0\u30fc\u30bc\u306e\u81f3\u9069\u6e29\u5ea6\u306f, 60\u2103\u3067\u3042\u308a, \u81f3\u9069pH\u306f, 5.5\u3067\u3042\u3063\u305f\u3002\u307e\u305f\u3053\u306e\u9175\u7d20(20\u03bcg)\u306f, \u30ac\u30e9\u30af\u30c8\u30fc\u30b9\u306b\u3088\u308a\u963b\u5bb3\u3092\u53d7\u3051, \u30ac\u30e9\u30af\u30c8\u30fc\u30b90.01M\u6fc3\u5ea6\u306750%, 0.02M\u6fc3\u5ea6\u306730%\u4ee5\u4e0b\u306b\u9175\u7d20\u6d3b\u6027\u304c\u4f4e\u4e0b\u3057\u305f\u3002\u307e\u305f\u3053\u306e\u963b\u5bb3\u4f5c\u7528\u306f, pH5.5\u3067\u6700\u3082\u5f37\u304b\u3063\u305f\u3002", "subitem_description_type": "Other"}, {"subitem_description": "Acid invertase activity of sugar cane was high at the fouth internode from the top of the stalk, which was low in sugar concentration (7%per juice), relatively high in reducing sugar concentration (about 2%), and enough large in the lenth, and the diameter. This enzyme was purified from sugar cane juice using ammonium safate fractionation, DEAE cellulose, and Sephadex G-200 gel column chromatography with about 2,000 fold increase in specific activity. This purified enzyme preparation was found to be three bands by disc gel electrophoresis, and glycoprotein by staining the gel with periodic acid-Schiff reagent. The enzyme preparation hydrolysed sucrose, raffinose, p-nitrophenyl-\u03b1-D-galactopyranoside, p-nitrophenyl-2-aceto-amido-2-deoxy-\u03b2-D-glucopyranoside, and p-nitrophenyl-2-deoxy-\u03b2-D-galactopyranoside. Thermal stability of the enzyme preparation was different from the substrates used by enzyme assay, the enzyme was stable at less than 45\u2103 by using sucrose as a substrate, and at less than 55\u2103 by using raffinose. Reaction mixtures (45\u2103, 30min) of the enzyme preparatin and raffinose were found to contain raffinose, galactose, sucrose, and fructose by paper chromatography. These results suggested to be contained \u03b1-galactosidase as well as acid invertase in this enzyme preparation. This \u03b1-galactosidase activity was maximum at 60\u2103 and at pH 5.5 by using p-nitrophenyl-\u03b1-D-galactopyranoside as a substrate. The activity was inhibited by galactose, and losed 50% at the concentration of 0.01M, and 70% at 0.02M. The inhibition was maximum at pH 5.5.", "subitem_description_type": "Other"}, {"subitem_description": "\u7d00\u8981\u8ad6\u6587", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/4149"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8\u5b66\u8853\u5831\u544a"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueNumber": "26", "bibliographicPageEnd": "104", "bibliographicPageStart": "91"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "KJ00000161999.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2003159/files/KJ00000161999.pdf"}, "version_id": "ce2504c9-e78b-415f-b38d-2e2f96136e96"}]}, "item_title": "\u7518\u8517\u830e\u306e\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc\u3068\u03b1-\u30ac\u30e9\u30af\u30c8\u30b7\u30c0\u30fc\u30bc\u306b\u95a2\u3059\u308b\u7814\u7a76(\u8fb2\u82b8\u5316\u5b66\u79d1)", "item_type_id": "15", "owner": "1", "path": ["1642837911830", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/4149", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-02-14"}, "publish_date": "2008-02-14", "publish_status": "0", "recid": "2003159", "relation": {}, "relation_version_is_last": true, "title": ["\u7518\u8517\u830e\u306e\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc\u3068\u03b1-\u30ac\u30e9\u30af\u30c8\u30b7\u30c0\u30fc\u30bc\u306b\u95a2\u3059\u308b\u7814\u7a76(\u8fb2\u82b8\u5316\u5b66\u79d1)"], "weko_shared_id": -1}