{"_buckets": {"deposit": "61ebd2c6-a1e3-484f-8475-a7c8b035d156"}, "_deposit": {"id": "2003161", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2003161"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02003161", "sets": ["1642837911830", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "\u6ce1\u76db\u9eb9\u83cc\u306e D-\u30ed\u30a4\u30b7\u30f3\u9178\u5316\u9175\u7d20(\u8fb2\u82b8\u5316\u5b66\u79d1)", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "D-Leucine oxidase of Aspergillus awamori (Department of Agricultural Chemistry)", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u5f53\u5c71, \u6e05\u5584", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u4e0e\u90a3\u8987, \u548c\u96c4", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u77e5\u540d, \u5b9a\u4e00", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Toyama, Seizen", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Yonaha, Kazuo", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "China, Teiichi", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "\u6ce1\u76db\u9eb9\u83cc\u306eD-\u30ed\u30a4\u30b7\u30f3\u9178\u5316\u9175\u7d20\u6d3b\u6027\u3092\u8abf\u3079\u305f\u7d50\u679c, \u672c\u9175\u7d20\u6d3b\u6027\u304c\u5404\u7a2e\u6ce1\u76db\u9eb9\u83cc\u83cc\u682a\u306e\u7121\u7d30\u80de\u62bd\u51fa\u6db2\u4e2d\u306b\u5b58\u5728\u3059\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u305f\u3002\u6700\u3082\u9ad8\u3044\u9175\u7d20\u6d3b\u6027\u3092\u793a\u3057\u305fAspergillus awamori IAM 2112\u3092\u7528\u3044\u3066\u9175\u7d20\u306e\u6027\u8cea\u3092\u8abf\u3079\u305f\u3002\u672c\u83cc\u682a\u3092\u30da\u30d7\u30c8\u30f3\u30fc\u30b0\u30eb\u30b3\u30fc\u30b9\u57f9\u5730\u306748\u6642\u9593\u632f\u3068\u3046\u57f9\u990a\u3057\u3066\u5f97\u305f\u83cc\u4f53\u3067\u9ad8\u3044\u9175\u7d20\u6d3b\u6027\u304c\u5f97\u3089\u308c\u305f\u3002\u672c\u9175\u7d20\u306fD-\u30ed\u30a4\u30b7\u30f3\u3092\u9178\u5316\u3057\u3066\u03b1-\u30b1\u30c8\u30a4\u30bd\u30ab\u30d7\u30ed\u30f3\u9178\u306e\u751f\u6210\u53cd\u5fdc\u3092\u89e6\u5a92\u3057, D-\u30ed\u30a4\u30b7\u30f3\u306b\u5bfe\u3059\u308bkm\u5024\u306f10mM\u3067\u3042\u3063\u305f\u3002\u672c\u9175\u7d20\u306e\u53cd\u5fdc\u81f3\u9069pH\u306f8.0\u0026acd;9.0\u3067, \u53cd\u5fdc\u81f3\u9069\u6e29\u5ea6\u306f40\u2103\u3067\u3042\u3063\u305f\u3002", "subitem_description_type": "Other"}, {"subitem_description": "The activity of D-leucine oxidase was distributed in various strains of Aspergillus awamori. A high activity of the enzyme was found in Asp. awamori IAM 2112,and the strain was chosen for characterization of the enzyme. When the strain was grown on peptone-glucose medium with shaking for 48hr, the strong D-leucine oxidase activity of the cell-free extract was obtained. The enzyme catalyzed the oxidation of D-leucine to produce \u03b1-ketoisocaproate, and the Michaelis constant for D-leucine was 1\u00d7(10)^\u003c-3\u003eM. The enzyme had a maximum activity in the pH range of 8.0-9.0. The optimum temperature for the reaction was at 40\u2103.", "subitem_description_type": "Other"}, {"subitem_description": "\u7d00\u8981\u8ad6\u6587", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/4151"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8\u5b66\u8853\u5831\u544a"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueNumber": "26", "bibliographicPageEnd": "123", "bibliographicPageStart": "115"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "KJ00000162001.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2003161/files/KJ00000162001.pdf"}, "version_id": "17ec5778-f78a-44b1-971d-2e8a6d179b86"}]}, "item_title": "\u6ce1\u76db\u9eb9\u83cc\u306e D-\u30ed\u30a4\u30b7\u30f3\u9178\u5316\u9175\u7d20(\u8fb2\u82b8\u5316\u5b66\u79d1)", "item_type_id": "15", "owner": "1", "path": ["1642837911830", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/4151", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-02-14"}, "publish_date": "2008-02-14", "publish_status": "0", "recid": "2003161", "relation": {}, "relation_version_is_last": true, "title": ["\u6ce1\u76db\u9eb9\u83cc\u306e D-\u30ed\u30a4\u30b7\u30f3\u9178\u5316\u9175\u7d20(\u8fb2\u82b8\u5316\u5b66\u79d1)"], "weko_shared_id": -1}