{"_buckets": {"deposit": "4e4c55d7-b272-416a-a621-fd58d19a519a"}, "_deposit": {"id": "2003324", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2003324"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02003324", "sets": ["1642837909579", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "\u30aa\u30cb\u30d2\u30c8\u30c7\u306e\u6bd2\u7d20\u6210\u5206\u306b\u95a2\u3059\u308b\u7814\u7a76 : \u7b2c 1 \u5831 \u68d8\u76ae\u4e2d\u306b\u5b58\u5728\u3059\u308b\u6bd2\u7d20\u306e\u5206\u96e2\u3068 2,3 \u306e\u6027\u8cea\u306b\u3064\u3044\u3066(\u8fb2\u82b8\u5316\u5b66\u79d1)", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "Studies on the toxin in the spines of starfish Acanthaster planci : I. Isolation and some properties of the toxin found in spines (Department of Agricultural Chemistry)", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u5e73\u826f, \u60a6\u5b50", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u68da\u539f, \u61b2\u548c", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u8239\u6d25, \u52dd", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Taira, Etsuko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Tanahara, Norikazu", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Funatsu, Masaru", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "\u30aa\u30cb\u30d2\u30c8\u30c7\u306e\u68d8\u76ae\u4e2d\u306b\u5b58\u5728\u3059\u308b\u6bd2\u7d20\u3092\u786b\u5b89\u5206\u753b, \u7b49\u96fb\u70b9\u5206\u753b\u304a\u3088\u3073Sephadex G-75\u30ab\u30e9\u30e0\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u306a\u3069\u306e\u65b9\u6cd5\u306b\u3088\u308a\u5206\u96e2\u3057, \u305d\u308c\u305e\u308c\u306e\u753b\u5206\u306b\u304a\u3044\u3066, \u30de\u30a6\u30b9\u306b\u5bfe\u3059\u308b\u81f4\u6b7b\u4f5c\u7528\u304a\u3088\u3073\u6eb6\u8840\u4f5c\u7528\u3092\u6bd4\u8f03\u691c\u8a0e\u3057, 2,3\u306e\u7406\u5316\u5b66\u7684\u6027\u8cea\u306b\u3064\u3044\u3066\u8abf\u3079\u305f\u3002\u305d\u306e\u7d50\u679c, \u62bd\u51fa\u6db2\u304b\u3089\u786b\u5b89\u5206\u753b\u306b\u81f3\u308b\u6bd2\u6027\u306f\u7d045.5\u500d\u306b\u4e0a\u3063\u305f\u3002Sephadex\u30ab\u30e9\u30e0\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u307e\u3067\u306f, \u62bd\u51fa\u6db2\u304b\u3089\u7d042.2\u500d\u3067\u786b\u5b89\u5206\u753b\u306e\u6bb5\u968e\u3088\u308a\u3082, \u3080\u3057\u308d\u6bd2\u6027\u306e\u4f4e\u4e0b\u304c\u8a8d\u3081\u3089\u308c\u305f\u3051\u308c\u3069\u3082, \u6eb6\u8840\u4f5c\u7528\u306f, \u30b2\u30eb\u6ffe\u904e\u306e\u6bb5\u968e\u3067\u62bd\u51fa\u6db2\u306e\u6eb6\u8840\u6307\u6570\u306b\u5bfe\u3057\u3066\u7d041/14\u306b\u4f4e\u4e0b\u3057\u305f\u3002\u3053\u308c\u3089\u306e\u3053\u3068\u304b\u3089, \u672c\u5b9f\u9a13\u3067\u5206\u96e2\u3057\u305f\u6bd2\u7d20\u306f\u81f4\u6b7b\u4f5c\u7528\u3092\u6709\u3057, \u6eb6\u8840\u4f5c\u7528\u7269\u8cea\u3068\u306f\u7570\u306a\u308b\u6210\u5206\u3067\u306f\u306a\u3044\u304b\u3068\u8003\u3048\u3089\u308c\u305f\u3002\u3053\u306e\u6bd2\u7d20\u306f\u975e\u900f\u6790\u6027\u3067\u3042\u308a, 60\u2103\u3067\u5931\u6d3b\u3057, \u307e\u305fpH3\u4ee5\u4e0b, pH10\u4ee5\u4e0a\u3067\u5931\u6d3b\u3059\u308b\u3053\u3068\u304c\u8a8d\u3081\u3089\u308c, \u3055\u3089\u306b\u51cd\u7d50, \u89e3\u51cd\u3092\u304f\u308a\u8fd4\u3059\u3068\u5931\u6d3b\u3059\u308b\u3053\u3068\u304b\u3089, \u86cb\u767d\u69d8\u7269\u8cea\u3067\u3042\u308b\u3053\u3068\u304c\u63a8\u5b9a\u3055\u308c\u305f\u3002\u7d42\u308a\u306b, \u672c\u7814\u7a76\u306b\u4f7f\u7528\u3057\u305f\u30aa\u30cb\u30d2\u30c8\u30c7\u63a1\u53d6\u306b, \u591a\u5927\u306a\u5fa1\u5354\u529b\u3092\u304f\u3060\u3055\u3044\u307e\u3057\u305f\u672c\u90e8\u753a\u5f79\u6240\u7d4c\u6e08\u8ab2\u306e\u559c\u5c4b\u6b66\u7fa9\u548c\u6c0f, \u304a\u3088\u3073\u4eca\u5e30\u4ec1\u6751\u5f79\u6240\u7d4c\u6e08\u8ab2\u306e\u7686\u69d8\u306b\u6df1\u304f\u611f\u8b1d\u81f4\u3057\u307e\u3059\u3002\u307e\u305f, \u672c\u7814\u7a76\u306b\u5f53\u308a\u7d42\u59cb\u5fa1\u61c7\u5207\u306a\u308b\u5fa1\u6307\u5c0e\u3092\u8cdc\u308a\u307e\u3057\u305f\u4e5d\u5dde\u5927\u5b66\u8fb2\u5b66\u90e8\u8239\u6d25\u8ecd\u559c\u52a9\u6559\u6388\u304a\u3088\u3073\u6771\u5317\u5927\u5b66\u8fb2\u5b66\u90e8\u5b89\u5143\u5065\u52a9\u6559\u6388\u306b\u6df1\u751a\u306e\u8b1d\u610f\u3092\u8868\u3057\u307e\u3059\u3002", "subitem_description_type": "Other"}, {"subitem_description": "The toxin of starfish Acanthaster planci was isolated by ammonium sulfate fractionation, isoelectric focussing fractionation at pH4.7,and Sephadex G-75 column chromatography. The toxicity of the ammonium sulfate fraction was approximately 5.5 times that of the extract. However, the toxicity of the gel filtrated fraction was 2.2 times that of the extract. This decrease of toxicity was probably caused by an acid-treatment in the isoelectric focussing fractionation. Hemolysis of each fraction decreased in the order followed by the fractionation except the ammonium sulfate fraction. The hemolysis index of the gel filtrated fraction was 1/(14) times that of the extract. From these facts, it is suggested that the toxin isolated in the present experiment has lethal action and is different from the toxin that has hemolytic action. This toxin was non-dialyzable, and in thermal stability experiments it lost its activity at 60\u2103. On the pH stability, it lost its toxic activity at pH lower than 3.0 or higher than 10.0. In addition the toxin that was exposed to repetitive freezing (at 20\u2103) and thawing was non-active. From these observations, it is considered that starfish Acanthaster planci toxin is probably a protein-like substance.", "subitem_description_type": "Other"}, {"subitem_description": "\u7d00\u8981\u8ad6\u6587", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/4332"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8\u5b66\u8853\u5831\u544a"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueNumber": "22", "bibliographicPageEnd": "213", "bibliographicPageStart": "203"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "KJ00000162517.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2003324/files/KJ00000162517.pdf"}, "version_id": "e799e020-93e9-4d4e-b569-14123050b92f"}]}, "item_title": "\u30aa\u30cb\u30d2\u30c8\u30c7\u306e\u6bd2\u7d20\u6210\u5206\u306b\u95a2\u3059\u308b\u7814\u7a76 : \u7b2c 1 \u5831 \u68d8\u76ae\u4e2d\u306b\u5b58\u5728\u3059\u308b\u6bd2\u7d20\u306e\u5206\u96e2\u3068 2,3 \u306e\u6027\u8cea\u306b\u3064\u3044\u3066(\u8fb2\u82b8\u5316\u5b66\u79d1)", "item_type_id": "15", "owner": "1", "path": ["1642837909579", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/4332", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-02-14"}, "publish_date": "2008-02-14", "publish_status": "0", "recid": "2003324", "relation": {}, "relation_version_is_last": true, "title": ["\u30aa\u30cb\u30d2\u30c8\u30c7\u306e\u6bd2\u7d20\u6210\u5206\u306b\u95a2\u3059\u308b\u7814\u7a76 : \u7b2c 1 \u5831 \u68d8\u76ae\u4e2d\u306b\u5b58\u5728\u3059\u308b\u6bd2\u7d20\u306e\u5206\u96e2\u3068 2,3 \u306e\u6027\u8cea\u306b\u3064\u3044\u3066(\u8fb2\u82b8\u5316\u5b66\u79d1)"], "weko_shared_id": -1}