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  1. 紀要論文
  2. 琉球大学農学部学術報告
  3. 22号
  1. 部局別インデックス
  2. 農学部

オニヒトデの毒素成分に関する研究 : 第 1 報 棘皮中に存在する毒素の分離と 2,3 の性質について(農芸化学科)

http://hdl.handle.net/20.500.12000/4332
http://hdl.handle.net/20.500.12000/4332
cbbc0a0a-8688-44d2-8332-8e2f70a95b93
名前 / ファイル ライセンス アクション
KJ00000162517.pdf KJ00000162517.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2008-02-14
タイトル
タイトル オニヒトデの毒素成分に関する研究 : 第 1 報 棘皮中に存在する毒素の分離と 2,3 の性質について(農芸化学科)
言語 ja
作成者 平良, 悦子

× 平良, 悦子

ja 平良, 悦子

棚原, 憲和

× 棚原, 憲和

ja 棚原, 憲和

船津, 勝

× 船津, 勝

ja 船津, 勝

Taira, Etsuko

× Taira, Etsuko

en Taira, Etsuko

Tanahara, Norikazu

× Tanahara, Norikazu

en Tanahara, Norikazu

Funatsu, Masaru

× Funatsu, Masaru

en Funatsu, Masaru

アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
内容記述
内容記述タイプ Other
内容記述 オニヒトデの棘皮中に存在する毒素を硫安分画, 等電点分画およびSephadex G-75カラムクロマトグラフィーなどの方法により分離し, それぞれの画分において, マウスに対する致死作用および溶血作用を比較検討し, 2,3の理化学的性質について調べた。その結果, 抽出液から硫安分画に至る毒性は約5.5倍に上った。Sephadexカラムクロマトグラフィーまでは, 抽出液から約2.2倍で硫安分画の段階よりも, むしろ毒性の低下が認められたけれども, 溶血作用は, ゲル濾過の段階で抽出液の溶血指数に対して約1/14に低下した。これらのことから, 本実験で分離した毒素は致死作用を有し, 溶血作用物質とは異なる成分ではないかと考えられた。この毒素は非透析性であり, 60℃で失活し, またpH3以下, pH10以上で失活することが認められ, さらに凍結, 解凍をくり返すと失活することから, 蛋白様物質であることが推定された。終りに, 本研究に使用したオニヒトデ採取に, 多大な御協力をくださいました本部町役所経済課の喜屋武義和氏, および今帰仁村役所経済課の皆様に深く感謝致します。また, 本研究に当り終始御懇切なる御指導を賜りました九州大学農学部船津軍喜助教授および東北大学農学部安元健助教授に深甚の謝意を表します。
内容記述タイプ Other
内容記述 The toxin of starfish Acanthaster planci was isolated by ammonium sulfate fractionation, isoelectric focussing fractionation at pH4.7,and Sephadex G-75 column chromatography. The toxicity of the ammonium sulfate fraction was approximately 5.5 times that of the extract. However, the toxicity of the gel filtrated fraction was 2.2 times that of the extract. This decrease of toxicity was probably caused by an acid-treatment in the isoelectric focussing fractionation. Hemolysis of each fraction decreased in the order followed by the fractionation except the ammonium sulfate fraction. The hemolysis index of the gel filtrated fraction was 1/(14) times that of the extract. From these facts, it is suggested that the toxin isolated in the present experiment has lethal action and is different from the toxin that has hemolytic action. This toxin was non-dialyzable, and in thermal stability experiments it lost its activity at 60℃. On the pH stability, it lost its toxic activity at pH lower than 3.0 or higher than 10.0. In addition the toxin that was exposed to repetitive freezing (at 20℃) and thawing was non-active. From these observations, it is considered that starfish Acanthaster planci toxin is probably a protein-like substance.
内容記述タイプ Other
内容記述 紀要論文
出版者
言語 ja
出版者 琉球大学農学部
言語
言語 jpn
資源タイプ
資源タイプ departmental bulletin paper
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
識別子
識別子 http://hdl.handle.net/20.500.12000/4332
識別子タイプ HDL
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0370-4246
収録物識別子タイプ NCID
収録物識別子 AN00250548
収録物名
言語 ja
収録物名 琉球大学農学部学術報告
書誌情報
号 22, p. 203-213
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